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Questions and Answers
What is the purpose of subcellular fractionation?
What is the purpose of subcellular fractionation?
- To increase enzymic degradation in the cell
- To study the whole cell under a microscope
- To isolate one type of subcellular organelle from the rest of the cell contents (correct)
- To identify all the enzymes present in the cell
How are cells initially broken open in subcellular fractionation?
How are cells initially broken open in subcellular fractionation?
- Freezing at -80°C
- Homogenization in a blender or homogenizer (correct)
- Boiling in water
- Shaking vigorously by hand
Why is subcellular fractionation usually carried out at 4°C?
Why is subcellular fractionation usually carried out at 4°C?
- To prevent contamination of the sample
- To increase the size of subcellular organelles
- To speed up the process of fractionation
- To minimize enzymic degradation of the cell’s constituents (correct)
What is the purpose of straining the sample through muslin in subcellular fractionation?
What is the purpose of straining the sample through muslin in subcellular fractionation?
How are various subcellular organelles separated in differential velocity centrifugation?
How are various subcellular organelles separated in differential velocity centrifugation?
What forces are used to separate subcellular organelles in a centrifuge during differential velocity centrifugation?
What forces are used to separate subcellular organelles in a centrifuge during differential velocity centrifugation?
What is the purpose of fluorescence-activated cell sorter (FACS)?
What is the purpose of fluorescence-activated cell sorter (FACS)?
How are primary cultures different from secondary cultures?
How are primary cultures different from secondary cultures?
What is the main goal of subcellular fractionation?
What is the main goal of subcellular fractionation?
In differential velocity centrifugation, what separates the subcellular organelles?
In differential velocity centrifugation, what separates the subcellular organelles?
What organelles pellet at lower forces in a differential velocity centrifugation?
What organelles pellet at lower forces in a differential velocity centrifugation?
What principle is used in equilibrium density-gradient centrifugation to separate organelles?
What principle is used in equilibrium density-gradient centrifugation to separate organelles?
What is the purpose of using increasingly higher g forces for longer periods during cell fractionation?
What is the purpose of using increasingly higher g forces for longer periods during cell fractionation?
Which cellular component is pelleted first during the fractionation process?
Which cellular component is pelleted first during the fractionation process?
Which of the following centrifugation conditions would pellet mitochondria, peroxisomes, and chloroplasts (if present)?
Which of the following centrifugation conditions would pellet mitochondria, peroxisomes, and chloroplasts (if present)?
What is the final supernatant fraction obtained after the complete fractionation process?
What is the final supernatant fraction obtained after the complete fractionation process?
Which of the following cellular components would be found in the pellet obtained after centrifugation at 40,000g for 30 minutes?
Which of the following cellular components would be found in the pellet obtained after centrifugation at 40,000g for 30 minutes?
Which centrifugation step separates ribosomal subunits from the cytosolic fraction?
Which centrifugation step separates ribosomal subunits from the cytosolic fraction?
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