Steps of the CU Pathway (Type I Pili)

Individual pilin subunit is translocated to the periplasm via ______ pathway.

The pili proteins form disulfide bonds between their β-strands A and B catalyzed by periplasmic oxidoreductase ______.

The chaperone recognizes the deep hydrophobic channels present in the unstable pilin subunit and confers the early stage of stability by offering the chaperone’s strand to the hydrophobic channel in parallel to the F strand of the unstable pili subunit (Donor strand complementation, ______).

The chaperone-subunit complex targets the ______ protein to initiate the pili elongation.

The chaperone strand is replaced by the strand of the next subunit during the assembly mechanism of pili. This is achieved by the exchange of the G1 strand with the (Nte) of the next subunit (Donor strand ______).

The deep hydrophobic channel on the pilin surface is formed during the formation of disulfide bonds between β-strands A and B catalyzed by periplasmic oxidoreductase _____

Donor strand complementation (DSC) confers the early stage of stability to the unstable pilin subunit by offering the ______’s strand to the hydrophobic channel in parallel to the F strand of the unstable pili subunit. The ______ recognizes the deep hydrophobic channels present in the unstable pilin subunit and confers stability by offering the ______’s strand to the hydrophobic channel in parallel to the F strand of the unstable pili subunit.

The chaperone-subunit complex targets the usher protein to initiate the pili elongation. The chaperone-subunit complex targets the _____ protein to initiate the pili elongation.

During the assembly mechanism of pili, the chaperone strand is replaced by the strand of the ______ subunit. This is achieved by the exchange of the G1 strand with the (Nte) of the ______ subunit (Donor strand exchange).

Individual pilin subunit is translocated to the periplasm via _____ pathway before forming disulfide bonds between β-strands A and B.