Stem Cells in Medicine

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Questions and Answers

What is the primary goal of blocking the self-renewal pathway in stem cells?

  • To inhibit transcription factor activity
  • To induce differentiation into a specific cell type (correct)
  • To promote cell proliferation
  • To initiate genetic alterations

Which of the following transcription factors is involved in the induction of pluripotency in stem cells?

  • Stats 3
  • Nanog (correct)
  • KLF4
  • SOX2

What is the purpose of using short interference RNA (siRNA) in stem cell therapies?

  • To induce gene expression
  • To inhibit gene expression (correct)
  • To initiate genetic alterations
  • To promote cell proliferation

How are genes typically transferred into cultured cells for stem cell-based therapies?

<p>Through direct injection into the cell (A)</p> Signup and view all the answers

What is the primary advantage of using stem cell-based therapies for autoimmune disease treatment?

<p>To avoid the need for immunosuppressive therapy (D)</p> Signup and view all the answers

What is the role of OCT-3/4 in stem cell pluripotency?

<p>To induce pluripotency (D)</p> Signup and view all the answers

What is the primary goal of using stem cells in cardiac repair?

<p>To regenerate damaged blood vessels and heart muscle (B)</p> Signup and view all the answers

What technique is used to manipulate the DNA of stem cells?

<p>CRISPR-Cas9 (D)</p> Signup and view all the answers

What is a characteristic of pluripotent stem cells?

<p>They can differentiate into any cell type (B)</p> Signup and view all the answers

What is the role of siRNA in stem cell therapy?

<p>To block the transcription of genes involved in disease (D)</p> Signup and view all the answers

What is the goal of using stem cells in autoimmune disease treatment?

<p>To block the autoimmune response (A)</p> Signup and view all the answers

What is the source of stem cells used in some therapeutic approaches?

<p>Human BM stem cells (D)</p> Signup and view all the answers

What happens when Caspase 8 is inhibited?

<p>Necroptosis is promoted (B)</p> Signup and view all the answers

What is the result of glucagon binding to its receptor on liver cells?

<p>Hydrolysis of triacylglycerols is enhanced (D)</p> Signup and view all the answers

What is the role of the inflammasome in the immune response?

<p>To enhance inflammation (A)</p> Signup and view all the answers

How do Ag-Ab complexes contribute to the immune response?

<p>By activating the complement system (C)</p> Signup and view all the answers

What is the result of UNF-kB activation?

<p>Cytokine production is stimulated (D)</p> Signup and view all the answers

What is the purpose of gene manipulation in stem cell therapy?

<p>To enhance stem cell pluripotency (B)</p> Signup and view all the answers

Flashcards

Stem Cell Differentiation

The process of inducing stem cells to specialize into a specific cell type.

Nanog

A transcription factor involved in maintaining the pluripotent state of stem cells.

siRNA in Stem Cell Therapy

A technique used to silence gene expression in stem cells, potentially preventing unwanted cell growth or disease development.

Gene Transfer in Stem Cell Therapy

A method of delivering genes into cultured cells for stem cell therapies, aiming to alter cell behavior or function.

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Stem Cells and Autoimmune Diseases

A major advantage of using stem cells for autoimmune disease treatment, as it avoids the need for drugs that suppress the immune system.

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OCT-3/4 in Stem Cell Pluripotency

A transcription factor essential for maintaining stem cell pluripotency, allowing them to differentiate into any cell type.

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Stem Cells in Cardiac Repair

A therapeutic strategy using stem cells to regenerate damaged heart muscle and blood vessels after heart attacks or other heart problems.

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CRISPR-Cas9 Technology

A powerful tool for precisely editing the DNA of stem cells, potentially correcting genetic defects or altering cell functions.

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Pluripotent Stem Cells

The ability of a stem cell to differentiate into any type of cell in the body.

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siRNA in Stem Cell Therapy

The role of siRNA in stem cell therapy, targeting specific genes involved in disease development, aiming to prevent or treat disease.

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Stem Cells and Autoimmune Diseases

The ultimate goal of using stem cells in autoimmune disease treatment is to restore normal immune function by replacing or reprogramming immune cells.

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Human BM Stem Cells

A source of stem cells used in therapeutic approaches, often utilized for bone marrow transplantation.

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Caspase 8 and Necroptosis

The inhibition of Caspase 8, a key protein in apoptosis (programmed cell death), leads to necroptosis, a different type of cell death.

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Glucagon and Fat Breakdown

The binding of glucagon to its receptor on liver cells triggers the breakdown of stored fats (triacylglycerols), releasing fatty acids for energy production.

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The Inflammasome

A multi-protein complex that plays a crucial role in triggering inflammatory responses, ultimately leading to the recruitment of immune cells.

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Ag-Ab Complexes and Complement System

These complexes activate the complement system, a cascade of proteins that helps to fight off pathogens and promote inflammation.

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NF-kB and Cytokine Production

The activation of NF-kB, a transcription factor, triggers the production of cytokines, signaling molecules that play a key role in inflammation and immune responses.

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Gene Manipulation for Stem Cell Therapy

The modification of genes in stem cells aims to enhance their pluripotency, allowing them to differentiate into a wider range of cell types for therapeutic purposes.

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