Staphylococci Identification

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5 Questions

What is a distinguishing characteristic of Staphylococcus saprophyticus compared to Staphylococcus epidermidis?

Novobiocin resistant

Which property would you not find in both Staphylococcus epidermidis and Staphylococcus saprophyticus?

Ferments mannitol

Which organism is most commonly associated with infections of prosthetic devices and IV catheters?

Staphylococcus epidermidis

Which of the following would incorrectly identify a sample as Staphylococcus saprophyticus?

Coagulase test positive

Where is Staphylococcus saprophyticus most commonly found as a normal microbiota?

Female genital tract and perineum

Study Notes

Staphylococcus epidermidis

  • Gram-positive, catalase-positive, coagulase-negative, urease-positive cocci that form clusters
  • Sensitive to novobiocin
  • Does not ferment mannitol, unlike Staphylococcus aureus which can ferment it
  • Normal microbiota of skin, can contaminate blood cultures
  • Infects prosthetic devices (e.g. hip implant, heart valve) and IV catheters by producing adherent biofilms

Staphylococcus saprophyticus

  • Gram-positive, catalase-positive, coagulase-negative, urease-positive cocci that form clusters
  • Resistant to novobiocin
  • Normal microbiota of female genital tract and perineum
  • Second most common cause of uncomplicated UTI in young females, after E coli

Identify the characteristics of Staphylococcus epidermidis and Staphylococcus saprophyticus, including their Gram reaction, catalase and coagulase production, and ability to ferment mannitol.

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