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Questions and Answers
What is the intended use of the reagent in the Stanbio Glucose LiquiColor®?
For the quantitative determination of glucose in serum, plasma, or CSF.
Excessive levels of ascorbic acid can produce falsely low glucose values.
True
What is one of the precautions regarding the handling of the reagent?
The reagent contains sodium azide, which may be toxic if ingested.
What should be done before adding samples to the glucose reagent?
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Hyperglycemia may occur as a result of __________.
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According to the document, what is the storage requirement for the reagent?
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What factors are to be considered for glucose assays?
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Study Notes
Stanbio Glucose LiquiColor® (Oxidase) Procedure No. 1070
- Intended use is for quantitative determination of glucose in serum, plasma or CSF
- Reagent contains sodium azide
- Reagent is for in vitro diagnostics, normal lab handling precautions should be taken
- Reagent is ready to use
- The reagent is stable up to the end of the expiration date, if stored correctly at 2-8 °C
- Do not freeze reagent!
- Measurement is not influenced by reagent color changes as long as the absorbance is less than 0.80 at 500 nm in a 1 cm lightpath
- Discard reagent if it contains particulate matter
- Standard is stable up to the end of its expiration date when stored at 2-8 °C
Interfering Substances
- High levels of ascorbic acid can produce falsely low glucose values
Automated Procedure
- Consult your instrument manual for programming instructions
- Specific programming applications for most automated analyzers are available from Stanbio's Technical Service Department
Manual Procedure
- Warm reagent to ambient temperature, zero spectrophotometer at 500 nm with distilled water
- Add 1.0 mL reagent to cuvettes/test tubes and warm to 37 °C for 5 minutes
- Add 10 μL (0.010 mL) of sample to respective cuvette/test tube, mix gently and return to 37 °C incubation
- After 5 minutes of incubation, read and record the absorbance of all samples
- Linearity to 500 mg/dL can be achieved by increasing the reagent volume if desired
Summary and Principle
- Accurate estimation of glucose is important for diagnosis and management of hyperglycemia and hypoglycemia
- Hyperglycemia may occur due to diabetes mellitus, IV glucose fluids, severe stress, or cerebrovascular accidents
- Hypoglycemia may be the result of an insulinoma, insulin administration, inborn error of carbohydrate metabolism, or fasting
- Glucose oxidase methodology was introduced by Keilin and Hartree in 1948
- Keston later reported the use of the combined methodology
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Description
This quiz covers the Stanbio Glucose LiquiColor® (Oxidase) Procedure No. 1070, which is intended for the quantitative determination of glucose in serum, plasma, or CSF. It includes information about reagent preparation, storage requirements, potential interfering substances, and automated procedure instructions. Essential knowledge for laboratory professionals working with glucose diagnostics is emphasized.
