Stanbio Glucose LiquiColor® Procedure Overview

Study Notes

Intended use is for quantitative determination of glucose in serum, plasma or CSF
Reagent contains sodium azide
Reagent is for in vitro diagnostics, normal lab handling precautions should be taken
Reagent is ready to use
The reagent is stable up to the end of the expiration date, if stored correctly at 2-8 °C
Do not freeze reagent!
Measurement is not influenced by reagent color changes as long as the absorbance is less than 0.80 at 500 nm in a 1 cm lightpath
Discard reagent if it contains particulate matter
Standard is stable up to the end of its expiration date when stored at 2-8 °C

Consult your instrument manual for programming instructions
Specific programming applications for most automated analyzers are available from Stanbio's Technical Service Department

Warm reagent to ambient temperature, zero spectrophotometer at 500 nm with distilled water
Add 1.0 mL reagent to cuvettes/test tubes and warm to 37 °C for 5 minutes
Add 10 μL (0.010 mL) of sample to respective cuvette/test tube, mix gently and return to 37 °C incubation
After 5 minutes of incubation, read and record the absorbance of all samples
Linearity to 500 mg/dL can be achieved by increasing the reagent volume if desired

Accurate estimation of glucose is important for diagnosis and management of hyperglycemia and hypoglycemia
Hyperglycemia may occur due to diabetes mellitus, IV glucose fluids, severe stress, or cerebrovascular accidents
Hypoglycemia may be the result of an insulinoma, insulin administration, inborn error of carbohydrate metabolism, or fasting
Glucose oxidase methodology was introduced by Keilin and Hartree in 1948
Keston later reported the use of the combined methodology