10 Questions
What is the role of the 2'-OH on the branch site in the splicing reaction?
It conducts a nucleophilic attack on the phosphoryl group of the 5' splice site
What is the result of the nucleophilic attack by the 3'-OH of the 5' exon?
The two exons are joined
What is the function of the phosphoryl group of the 5' splice site?
It is the site of nucleophilic attack by the 2'-OH of the branch site
What is the final product of the splicing reaction?
A mature mRNA with two exons
What is the fate of the intron during the splicing reaction?
It is liberated from the mRNA
What is the immediate result of the nucleophilic attack by the 2'-OH on the branch site A?
Cleavage of the phosphodiester bond at the 5' splice site
Which group is involved in the formation of the intermediate in the first step of the splicing reaction?
2'-OH of the branch site A
What is the consequence of the nucleophilic attack by the 3'-OH of the 5' exon?
Formation of a phosphodiester bond between the two exons
What is the role of the phosphoryl group of the 5' splice site in the splicing reaction?
It is attacked by the 2'-OH of the branch site A
What is the outcome of the second step of the splicing reaction?
The intron is released, and the two exons are joined
Learn about the process of splicing reaction which involves nucleophilic attacks at the branch site and splice sites, leading to the joining of exons and removal of introns. This overview covers the key steps involved in the splicing process.
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