Splicing Reaction Mechanism Overview
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Questions and Answers

What is the role of the 2'-OH on the branch site in the splicing reaction?

  • It conducts a nucleophilic attack on the 3' splice site
  • It is involved in the joining of the two exons
  • It conducts a nucleophilic attack on the phosphoryl group of the 5' splice site (correct)
  • It is responsible for liberating the intron

    • What is the result of the nucleophilic attack by the 3'-OH of the 5' exon?

  • The branch site is formed
  • The 5' splice site is formed
  • The intron is liberated
  • The two exons are joined (correct)

    • What is the function of the phosphoryl group of the 5' splice site?

  • It is the site of nucleophilic attack by the 2'-OH of the branch site (correct)
  • It is responsible for the liberation of the intron
  • It is involved in the joining of the two exons
  • It is involved in the formation of the 3' splice site

    • What is the final product of the splicing reaction?

    <p>A mature mRNA with two exons</p> Signup and view all the answers

    What is the fate of the intron during the splicing reaction?

    <p>It is liberated from the mRNA</p> Signup and view all the answers

    What is the immediate result of the nucleophilic attack by the 2'-OH on the branch site A?

    <p>Cleavage of the phosphodiester bond at the 5' splice site</p> Signup and view all the answers

    Which group is involved in the formation of the intermediate in the first step of the splicing reaction?

    <p>2'-OH of the branch site A</p> Signup and view all the answers

    What is the consequence of the nucleophilic attack by the 3'-OH of the 5' exon?

    <p>Formation of a phosphodiester bond between the two exons</p> Signup and view all the answers

    What is the role of the phosphoryl group of the 5' splice site in the splicing reaction?

    <p>It is attacked by the 2'-OH of the branch site A</p> Signup and view all the answers

    What is the outcome of the second step of the splicing reaction?

    <p>The intron is released, and the two exons are joined</p> Signup and view all the answers

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