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Splicing Reaction Mechanism Overview

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Questions and Answers

What is the role of the 2'-OH on the branch site in the splicing reaction?

It conducts a nucleophilic attack on the 3' splice site
It is involved in the joining of the two exons
It conducts a nucleophilic attack on the phosphoryl group of the 5' splice site (correct)
It is responsible for liberating the intron

What is the result of the nucleophilic attack by the 3'-OH of the 5' exon?

The branch site is formed
The 5' splice site is formed
The intron is liberated
The two exons are joined (correct)

What is the function of the phosphoryl group of the 5' splice site?

It is the site of nucleophilic attack by the 2'-OH of the branch site (correct)
It is responsible for the liberation of the intron
It is involved in the joining of the two exons
It is involved in the formation of the 3' splice site

What is the final product of the splicing reaction?

A mature mRNA with two exons (B) Signup and view all the answers

What is the fate of the intron during the splicing reaction?

It is liberated from the mRNA (B) Signup and view all the answers

What is the immediate result of the nucleophilic attack by the 2'-OH on the branch site A?

Cleavage of the phosphodiester bond at the 5' splice site (A) Signup and view all the answers

Which group is involved in the formation of the intermediate in the first step of the splicing reaction?

2'-OH of the branch site A (C) Signup and view all the answers

What is the consequence of the nucleophilic attack by the 3'-OH of the 5' exon?

Formation of a phosphodiester bond between the two exons (D) Signup and view all the answers

What is the role of the phosphoryl group of the 5' splice site in the splicing reaction?

It is attacked by the 2'-OH of the branch site A (D) Signup and view all the answers

What is the outcome of the second step of the splicing reaction?

The intron is released, and the two exons are joined (A) Signup and view all the answers