Questions and Answers
What is the purpose of a reference cuvette in spectrophotometry?
To calibrate the instrument
In spectrophotometry, what does the Beer-Lambert law describe?
The relationship between light intensity and absorbance
What is the purpose of a monochromator in a spectrophotometer?
To filter out unwanted light
Which type of sample is unsuitable for analysis using a spectrophotometer?
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What is the relationship between absorbance and concentration in spectrophotometry?
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Study Notes
Spectrophotometry Basics
- A reference cuvette is used to calibrate the instrument in spectrophotometry.
Beer-Lambert Law
- The Beer-Lambert law describes the relationship between light intensity and absorbance.
Monochromator Function
- A monochromator in a spectrophotometer filters out unwanted light.
Sample Suitability
- Opaque samples are unsuitable for analysis using a spectrophotometer.
Absorbance and Concentration
- In spectrophotometry, absorbance is directly proportional to concentration.
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