Spectrophotometry and Beer's Law

The scattering coefficient quantifies the amount of light that is scattered by a medium, influencing how much light can penetrate through it.

The refractive index determines how much light bends when entering a new medium, affecting its speed and direction of propagation.

The absorption coefficient is dependent on the frequency of the photon, with certain frequencies being absorbed more efficiently than others.

<p>Molecular scattering affects how light interacts with particles in a medium, impacting how much light is either transmitted or absorbed.</p> Signup and view all the answers

<p>The absorption cross-section measures the likelihood of a photon being absorbed by a particle, relating the incident light's intensity to the absorption rate.</p> Signup and view all the answers

<p>$SO_2$ (%) is derived from the ratio of $C_{H_2O_2}$ to the sum of $C_{H_2O_2}$ and $C_{Hb}$, indicating that higher concentrations of $C_{H_2O_2}$ will yield a higher percentage of $SO_2$.</p> Signup and view all the answers

<p>The absorption coefficient $ ext{μ_a(λ)}$ quantifies how much light of a specific wavelength is absorbed by a substance, directly influencing the detected intensity and the accuracy of concentration measurements.</p> Signup and view all the answers

<p>Increasing the optical path length $d$ results in a lower detected intensity $I_L(λ)$ due to a greater extent of absorption, as indicated by the exponential decay in the equation.</p> Signup and view all the answers

<p>Rearranging gives $ ext{I_L(λ)} = ext{I_0(λ)} imes exp(- ext{μ_a(λ)}d)$, indicating how the detected intensity depends on the incident intensity and absorption characteristics of the substance.</p> Signup and view all the answers

<p>Variations in $I_0(λ)$ can lead to inconsistent results in detected intensity $I_L(λ)$, potentially compromising the accuracy of concentration calculations and the interpretability of absorption spectra.</p> Signup and view all the answers