Questions and Answers
What is the primary principle behind size exclusion chromatography?
It is based on differences in sizes of molecules.
How does the porous nature of the stationary phase affect small molecules in size exclusion chromatography?
Small molecules can enter the pores and are retained longer.
In size exclusion chromatography, what happens to larger molecules during the process?
Larger molecules go around the porous stationary phase and elute quickly.
What role does the size of the molecules play in their separation during size exclusion chromatography?
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Why is size exclusion chromatography particularly useful for separating proteins or polymers?
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What unique feature distinguishes size exclusion chromatography from the other three mechanisms?
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What is the primary factor that determines the separation in size exclusion chromatography?
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What is the main application of size exclusion chromatography in laboratory practices?
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What type of interactions between solutes and the stationary phase are absent in size exclusion chromatography?
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What category of chromatographic methods does size exclusion chromatography NOT belong to?
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Study Notes
Separation by Size Exclusion
- Distinct from other separation mechanisms, focusing solely on size.
- Involves neither specific nor nonspecific interactions between solutes and stationary phase.
- Effective separation is determined by pore sizes within the stationary phase.
- Control of separation can be achieved by adjusting pore sizes.
- Not categorized as an analytical separation method; rather, it serves practical purposes.
- Primarily used for sample preparation and removing unwanted solutes before analytical processes.
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Description
This quiz covers the principles of size exclusion chromatography, a separation technique that relies on differences in molecular sizes. It explains how smaller molecules are retained longer in the stationary phase, while larger ones elute quickly.
