Podcast
Questions and Answers
What is the formula to calculate channel density?
What is the formula to calculate channel density?
In the context of channel density, what does the term 'gating current measurements' refer to?
In the context of channel density, what does the term 'gating current measurements' refer to?
Considering the data provided, which type of channel has the highest conductance?
Considering the data provided, which type of channel has the highest conductance?
What is the lower limit for channel density in excitable cells based on membrane charging during action potentials?
What is the lower limit for channel density in excitable cells based on membrane charging during action potentials?
Signup and view all the answers
How is the number of channels per unit area estimated in terms of channel density calculations?
How is the number of channels per unit area estimated in terms of channel density calculations?
Signup and view all the answers
Based on the given data, which type of preparation channel has the highest number per μm^2?
Based on the given data, which type of preparation channel has the highest number per μm^2?
Signup and view all the answers
What is the lower limit of channel density per μm2 based on the information provided?
What is the lower limit of channel density per μm2 based on the information provided?
Signup and view all the answers
In single channel current measurements, what does 'ip = gp (Vm-Ep)' represent?
In single channel current measurements, what does 'ip = gp (Vm-Ep)' represent?
Signup and view all the answers
What is the significance of the duration of an action potential in relation to ion movement through channels?
What is the significance of the duration of an action potential in relation to ion movement through channels?
Signup and view all the answers
Which patch clamp configuration involves recording while fixing the membrane potential?
Which patch clamp configuration involves recording while fixing the membrane potential?
Signup and view all the answers
What does 'stochastic in opening and closing but unitary in amplitude' refer to in single channel currents?
What does 'stochastic in opening and closing but unitary in amplitude' refer to in single channel currents?
Signup and view all the answers
What is the conductance value calculated for the channel pore with a radius of 0.5nm and a length of 5nm?
What is the conductance value calculated for the channel pore with a radius of 0.5nm and a length of 5nm?
Signup and view all the answers
What is the impact of access resistance on single channel conductance in the example discussed?
What is the impact of access resistance on single channel conductance in the example discussed?
Signup and view all the answers
How does unequal ion distributions affect the current-voltage relationship in the context of single channel recording?
How does unequal ion distributions affect the current-voltage relationship in the context of single channel recording?
Signup and view all the answers
What does the slope of an I-V curve represent in the context of single channel current-voltage relation?
What does the slope of an I-V curve represent in the context of single channel current-voltage relation?
Signup and view all the answers
What can be inferred about the single potassium channel's conductance when compared with the calculated conductance of the channel pore?
What can be inferred about the single potassium channel's conductance when compared with the calculated conductance of the channel pore?
Signup and view all the answers
What happens at around -76mV based on the discussion in single channel current-voltage relation?
What happens at around -76mV based on the discussion in single channel current-voltage relation?
Signup and view all the answers