Single Channel Current and Measurement Quiz
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Questions and Answers

What is the formula to calculate channel density?

  • Macroscopic conductance / Surface area
  • Single-channel conductance / Macroscopic conductance (correct)
  • Surface area / Gating current
  • Single-channel conductance / Membrane capacitance
  • In the context of channel density, what does the term 'gating current measurements' refer to?

  • Calculating the number of channels per unit area
  • Measuring the current associated with channel opening and closing (correct)
  • Estimating channel density based on membrane capacitance
  • Measuring the conductance of each channel
  • Considering the data provided, which type of channel has the highest conductance?

  • Squid giant axon sodium channel
  • Frog skeletal potassium channel
  • Rat node sodium channel
  • Mammalian BK potassium channel (correct)
  • What is the lower limit for channel density in excitable cells based on membrane charging during action potentials?

    <p>10-15C/μm2</p> Signup and view all the answers

    How is the number of channels per unit area estimated in terms of channel density calculations?

    <p>Dividing single-channel conductance by surface area</p> Signup and view all the answers

    Based on the given data, which type of preparation channel has the highest number per μm^2?

    <p>Mammalian BK potassium channel</p> Signup and view all the answers

    What is the lower limit of channel density per μm2 based on the information provided?

    <p>1 channel</p> Signup and view all the answers

    In single channel current measurements, what does 'ip = gp (Vm-Ep)' represent?

    <p>The conductance of the channel</p> Signup and view all the answers

    What is the significance of the duration of an action potential in relation to ion movement through channels?

    <p>It determines the number of ions moved per channel</p> Signup and view all the answers

    Which patch clamp configuration involves recording while fixing the membrane potential?

    <p>Whole-cell</p> Signup and view all the answers

    What does 'stochastic in opening and closing but unitary in amplitude' refer to in single channel currents?

    <p>Consistency in the current passing through each ion channel</p> Signup and view all the answers

    What is the conductance value calculated for the channel pore with a radius of 0.5nm and a length of 5nm?

    <p>63 pS</p> Signup and view all the answers

    What is the impact of access resistance on single channel conductance in the example discussed?

    <p>Reduces it to 54 pS</p> Signup and view all the answers

    How does unequal ion distributions affect the current-voltage relationship in the context of single channel recording?

    <p>Results in non-linear I-V relation</p> Signup and view all the answers

    What does the slope of an I-V curve represent in the context of single channel current-voltage relation?

    <p>Permeability of the channel</p> Signup and view all the answers

    What can be inferred about the single potassium channel's conductance when compared with the calculated conductance of the channel pore?

    <p>Lower than 63 pS</p> Signup and view all the answers

    What happens at around -76mV based on the discussion in single channel current-voltage relation?

    <p>Non-linear I-V relation due to unequal ion distributions</p> Signup and view all the answers

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