Sickle Solubility Test (SST)
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Questions and Answers

What is the purpose of the Sickle Solubility Test (SST)?

  • To detect the presence of white blood cells
  • To identify the total protein content in the blood
  • To screen for the presence of sickling Hb (correct)
  • To measure the pH of the blood sample

What causes the solution in the Sickle Solubility Test to remain turbid?

  • Formation of neumatic liquid crystals
  • Lysing of RBCs
  • Reduced solubility of HbS (correct)
  • Presence of HbA

How is the presence of HbA detected in the Sickle Solubility Test?

  • Turbid red solution
  • Lysis of RBCs
  • Clear red solution (correct)
  • Formation of neumatic liquid crystals

Which of the following statements is true about sickle cell trait (Hb-AS)?

<p>It can lead to hematuria due to kidney papillae necrosis. (D)</p> Signup and view all the answers

What is the typical result of the sickling test after overnight incubation for individuals with sickle cell trait?

<p>Positive (B)</p> Signup and view all the answers

Which of the following best describes the appearance of RBCs in individuals with sickle cell trait on the peripheral blood film?

<p>Normal appearance of RBCs with few target cells. (B)</p> Signup and view all the answers

Which type of thalassemia is characterized by a reduced rate of synthesis of α chains?

<p>Alpha thalassemia (C)</p> Signup and view all the answers

In thalassemias, the condition is classified into α and β types based on the affected:

<p>Globin chain type (C)</p> Signup and view all the answers

What is the main genetic cause of thalassemias?

<p>Reduced rate of synthesis α or β chains (C)</p> Signup and view all the answers

Study Notes

Sickle Solubility Test (SST)

  • Purpose: Detects the presence of hemoglobin S (HbS) in blood samples
  • Solution remains turbid due to the presence of hemoglobin S (HbS) or other abnormal hemoglobins
  • Presence of HbA is detected by the solution remaining clear or becoming clear after adding a reducing agent

Sickle Cell Trait (Hb-AS)

  • Characterized by the presence of both HbA and HbS hemoglobins
  • Typical result of the sickling test after overnight incubation: partially sickled or normal red blood cell morphology
  • Appearance of RBCs on peripheral blood film: usually normal, but may show rare sickle cells or target cells

Thalassemias

  • α-Thalassemia is characterized by a reduced rate of synthesis of α chains
  • Classified into α and β types based on the affected globin chain of hemoglobin
  • Main genetic cause: mutations leading to reduced or absent synthesis of α or β globin chains

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Description

Learn about the Sickle Solubility Test (SST) used to screen for the presence of sickling Hb. Understand the procedure that involves lysing RBCs and reducing Hb using sodium saponin hydrosulfite in a phosphate buffer.

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