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Questions and Answers
What is the primary principle behind separating cream from milk using a centrifuge?
What is the primary principle behind separating cream from milk using a centrifuge?
What is a disadvantage of using traditional methods to separate cream from milk before the invention of centrifuges?
What is a disadvantage of using traditional methods to separate cream from milk before the invention of centrifuges?
In a centrifuge, what happens to the components of a mixture during the spinning process?
In a centrifuge, what happens to the components of a mixture during the spinning process?
What is likely to happen if freshly collected blood is placed in a centrifuge?
What is likely to happen if freshly collected blood is placed in a centrifuge?
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How does the use of a separating funnel differ from that of a centrifuge in separating oil and water?
How does the use of a separating funnel differ from that of a centrifuge in separating oil and water?
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What is the primary purpose of using a separating funnel?
What is the primary purpose of using a separating funnel?
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Which separation technique utilizes a rotational force for separation?
Which separation technique utilizes a rotational force for separation?
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What occurs to the less dense liquid when using a separating funnel?
What occurs to the less dense liquid when using a separating funnel?
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After centrifuging a mixture, what is the name of the solution that remains on top of the pellet?
After centrifuging a mixture, what is the name of the solution that remains on top of the pellet?
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Which separation technique would be best suited for separating a heterogeneous mixture of sand and salt?
Which separation technique would be best suited for separating a heterogeneous mixture of sand and salt?
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What happens to the particles in a centrifuge during centrifugation?
What happens to the particles in a centrifuge during centrifugation?
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Which of the following separation techniques is specifically used for separating colored substances in a mixture?
Which of the following separation techniques is specifically used for separating colored substances in a mixture?
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When performing decantation, what is typically removed?
When performing decantation, what is typically removed?
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What are some separation techniques that have been learned so far?
What are some separation techniques that have been learned so far?
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How does a separating funnel work?
How does a separating funnel work?
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What forms when a mixture is spun in a centrifuge?
What forms when a mixture is spun in a centrifuge?
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The less dense liquid sinks to the bottom in a separating funnel.
The less dense liquid sinks to the bottom in a separating funnel.
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What happens when freshly collected blood is placed in a centrifuge?
What happens when freshly collected blood is placed in a centrifuge?
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What happens if freshly collected milk is placed in a centrifuge?
What happens if freshly collected milk is placed in a centrifuge?
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A mixture of oil and water can be separated using a ______.
A mixture of oil and water can be separated using a ______.
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Match the following items with their description:
Match the following items with their description:
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What is the primary function of a centrifuge?
What is the primary function of a centrifuge?
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Centrifuging separates components of a mixture by adding heat to the process.
Centrifuging separates components of a mixture by adding heat to the process.
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Describe the process of separating a mixture of oil and water using a separating funnel.
Describe the process of separating a mixture of oil and water using a separating funnel.
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Which separation technique is used to separate a solid from a liquid by passing the mixture through a barrier that allows only the liquid to pass?
Which separation technique is used to separate a solid from a liquid by passing the mixture through a barrier that allows only the liquid to pass?
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A ______ is used to accelerate the separation of components in a liquid mixture by spinning it at high speeds.
A ______ is used to accelerate the separation of components in a liquid mixture by spinning it at high speeds.
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Match the following processes with their descriptions:
Match the following processes with their descriptions:
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A separating funnel can be used to separate two liquids that mix together.
A separating funnel can be used to separate two liquids that mix together.
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What is the term for the liquid that remains on top after centrifuging a mixture?
What is the term for the liquid that remains on top after centrifuging a mixture?
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Which component would form the pellet when freshly collected blood is put in a centrifuge?
Which component would form the pellet when freshly collected blood is put in a centrifuge?
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The process of using a machine to spin a mixture and separate particles based on density is called ______.
The process of using a machine to spin a mixture and separate particles based on density is called ______.
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Centrifuging milk could lead to the cream separating and floating on top of the milk.
Centrifuging milk could lead to the cream separating and floating on top of the milk.
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Match the separation technique with its description:
Match the separation technique with its description:
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What happens when freshly collected milk is placed in a centrifuge?
What happens when freshly collected milk is placed in a centrifuge?
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Before the invention of centrifuges, separation of cream from milk relied on letting the milk ______.
Before the invention of centrifuges, separation of cream from milk relied on letting the milk ______.
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Which separation technique would be most effective for separating sand from salt?
Which separation technique would be most effective for separating sand from salt?
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What are two benefits of using a centrifugal separator compared to traditional methods of separation?
What are two benefits of using a centrifugal separator compared to traditional methods of separation?
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Oil is denser than water and will sink when placed into a separating funnel.
Oil is denser than water and will sink when placed into a separating funnel.
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What happens to the more dense particles when a mixture is spun in a centrifuge?
What happens to the more dense particles when a mixture is spun in a centrifuge?
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In a separating funnel, the ______ liquid can be tapped off from the bottom.
In a separating funnel, the ______ liquid can be tapped off from the bottom.
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Which technique involves using a net to remove floating plastic from water?
Which technique involves using a net to remove floating plastic from water?
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What distinguishes a colloid from a suspension in terms of particle visibility?
What distinguishes a colloid from a suspension in terms of particle visibility?
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Define an emulsion and provide an example.
Define an emulsion and provide an example.
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Explain the Tyndall effect and how it can be used to differentiate between a colloid and a solution.
Explain the Tyndall effect and how it can be used to differentiate between a colloid and a solution.
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List the main differences between colloids and suspensions in terms of stability and particle size.
List the main differences between colloids and suspensions in terms of stability and particle size.
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Why are homogeneous mixtures considered uniform, and how does this relate to the definition of a colloid?
Why are homogeneous mixtures considered uniform, and how does this relate to the definition of a colloid?
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What distinguishes a colloid from a suspension in terms of particle size?
What distinguishes a colloid from a suspension in terms of particle size?
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How do emulsifiers function in a combination of two immiscible liquids?
How do emulsifiers function in a combination of two immiscible liquids?
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What is the Tyndall effect and how does it relate to colloids?
What is the Tyndall effect and how does it relate to colloids?
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List two examples of colloids and explain their characteristics.
List two examples of colloids and explain their characteristics.
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What is the main difference between a gel and a sol within colloids?
What is the main difference between a gel and a sol within colloids?
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Explain why unhomogenized milk is not classified as an emulsion.
Explain why unhomogenized milk is not classified as an emulsion.
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Identify and describe two types of colloids based on their dispersed phase and dispersion medium.
Identify and describe two types of colloids based on their dispersed phase and dispersion medium.
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How does the particle size in colloids affect their stability compared to suspensions?
How does the particle size in colloids affect their stability compared to suspensions?
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What type of dispersion medium is found in foams?
What type of dispersion medium is found in foams?
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What is the size range of the particles in a colloid?
What is the size range of the particles in a colloid?
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How does the Tyndall Effect demonstrate the presence of colloidal particles?
How does the Tyndall Effect demonstrate the presence of colloidal particles?
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What distinguishes emulsions from other types of colloids?
What distinguishes emulsions from other types of colloids?
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In what way can suspensions be visually differentiated from colloids?
In what way can suspensions be visually differentiated from colloids?
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What type of dispersed phase is found in solid foams?
What type of dispersed phase is found in solid foams?
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What is the primary distinguishing feature between a colloid and a suspension regarding particle size?
What is the primary distinguishing feature between a colloid and a suspension regarding particle size?
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Can solutions exhibit the Tyndall Effect? Why or why not?
Can solutions exhibit the Tyndall Effect? Why or why not?
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What is a common example of a solid sol, and how is it classified?
What is a common example of a solid sol, and how is it classified?
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Which colloid type is formed when liquid droplets are suspended in gas?
Which colloid type is formed when liquid droplets are suspended in gas?
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What distinguishes an emulsion from other types of colloids?
What distinguishes an emulsion from other types of colloids?
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What is the role of emulsifiers in emulsions?
What is the role of emulsifiers in emulsions?
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How do colloids differ from solutions in terms of visibility of particles?
How do colloids differ from solutions in terms of visibility of particles?
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What is the Tyndall effect and how does it relate to colloids?
What is the Tyndall effect and how does it relate to colloids?
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Provide an example of a colloid and explain its classification.
Provide an example of a colloid and explain its classification.
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Why is unhomogenised milk not considered an emulsion?
Why is unhomogenised milk not considered an emulsion?
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What is an example of a colloid that is a foam, and how is it formed?
What is an example of a colloid that is a foam, and how is it formed?
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Describe the difference between emulsions and suspensions.
Describe the difference between emulsions and suspensions.
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What type of mixture is mayonnaise, and what two components classify it?
What type of mixture is mayonnaise, and what two components classify it?
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Identify a common misperception about colloids and provide clarification.
Identify a common misperception about colloids and provide clarification.
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What is the stationary phase and the mobile phase when separating black ink on paper using water?
What is the stationary phase and the mobile phase when separating black ink on paper using water?
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What term describes the way the solvent moves up the stationary phase?
What term describes the way the solvent moves up the stationary phase?
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How should the base line be drawn in chromatography and why?
How should the base line be drawn in chromatography and why?
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What does it suggest about a dye that moves a long way up the stationary phase in terms of its solubility?
What does it suggest about a dye that moves a long way up the stationary phase in terms of its solubility?
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What can you infer about the dyes that do not move far along the stationary phase regarding their affinity for it?
What can you infer about the dyes that do not move far along the stationary phase regarding their affinity for it?
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Where should the solvent front be marked on the chromatogram?
Where should the solvent front be marked on the chromatogram?
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When comparing the chromatograms of two different black ink pens, what differences might you observe?
When comparing the chromatograms of two different black ink pens, what differences might you observe?
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What is the formula to calculate the Rf value in chromatography?
What is the formula to calculate the Rf value in chromatography?
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If a different solvent like ethanol were used instead of water, would the Rf values be the same?
If a different solvent like ethanol were used instead of water, would the Rf values be the same?
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What characterizes a pure substance compared to a mixture?
What characterizes a pure substance compared to a mixture?
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Give an example of a pure substance and explain why it qualifies as such.
Give an example of a pure substance and explain why it qualifies as such.
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What is one method chemists use to separate mixtures, and how does it work?
What is one method chemists use to separate mixtures, and how does it work?
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Explain why mixtures can be physically separated.
Explain why mixtures can be physically separated.
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What happens to the properties of substances when they form a mixture?
What happens to the properties of substances when they form a mixture?
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Why can blood be considered a mixture rather than a pure substance?
Why can blood be considered a mixture rather than a pure substance?
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List the types of mixtures based on physical states.
List the types of mixtures based on physical states.
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What role does solubility play in separating mixtures?
What role does solubility play in separating mixtures?
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Describe how a copper sulfate solution can be separated into its components.
Describe how a copper sulfate solution can be separated into its components.
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In what ways can the physical properties of particles affect separation techniques?
In what ways can the physical properties of particles affect separation techniques?
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What is the main difference between sieving and filtration in terms of the types of materials they separate?
What is the main difference between sieving and filtration in terms of the types of materials they separate?
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In the context of mixtures, what are suspensions and how do they differ from solutions?
In the context of mixtures, what are suspensions and how do they differ from solutions?
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How would you use a sieve to separate a mixture of flour and coarse sand?
How would you use a sieve to separate a mixture of flour and coarse sand?
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Explain how decantation can be applied to separate a mixture of oil and water.
Explain how decantation can be applied to separate a mixture of oil and water.
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Describe a situation where filtration would be the most suitable method of separation.
Describe a situation where filtration would be the most suitable method of separation.
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Why is decantation not effective for mixtures with fine silt and water?
Why is decantation not effective for mixtures with fine silt and water?
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What role does particle size play in the effectiveness of sieving?
What role does particle size play in the effectiveness of sieving?
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How can the concepts of suspensions and solutions aid in choosing the right separation technique?
How can the concepts of suspensions and solutions aid in choosing the right separation technique?
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What are some limitations of using sieving for separation tasks?
What are some limitations of using sieving for separation tasks?
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What is the main difference between a suspension and a solution?
What is the main difference between a suspension and a solution?
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How does filtration separate solid particles from liquids?
How does filtration separate solid particles from liquids?
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In the green mix experiment, what happened to the blue and yellow particles when water was added?
In the green mix experiment, what happened to the blue and yellow particles when water was added?
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What role does filter paper play in the process of filtration?
What role does filter paper play in the process of filtration?
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Describe how sand and water can be separated using filtration.
Describe how sand and water can be separated using filtration.
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Explain the significance of sediment in a suspension.
Explain the significance of sediment in a suspension.
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What is the outcome when water is added to a mixture of chalk and water?
What is the outcome when water is added to a mixture of chalk and water?
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Why are solids like sand and chalk classified as insoluble?
Why are solids like sand and chalk classified as insoluble?
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How does a conical flask facilitate the collection of filtrate during filtration?
How does a conical flask facilitate the collection of filtrate during filtration?
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What happens to the insoluble particles in a suspension when allowed to sit undisturbed?
What happens to the insoluble particles in a suspension when allowed to sit undisturbed?
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What is the main difference between a suspension and a solution?
What is the main difference between a suspension and a solution?
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How does filtration work to separate mixtures?
How does filtration work to separate mixtures?
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Name a common example of a suspension.
Name a common example of a suspension.
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What role does the solvent play in a solution?
What role does the solvent play in a solution?
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Why can chalk and water be separated by filtration?
Why can chalk and water be separated by filtration?
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List two substances that are insoluble in water.
List two substances that are insoluble in water.
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What happens to a soluble substance when mixed with a solvent?
What happens to a soluble substance when mixed with a solvent?
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Name a physical separation technique that can remove impurities from a liquid.
Name a physical separation technique that can remove impurities from a liquid.
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How can you separate a mixture of oil and water?
How can you separate a mixture of oil and water?
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What is the importance of understanding solubility in separation techniques?
What is the importance of understanding solubility in separation techniques?
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Study Notes
Separation Techniques
- Sieving, Decanting, Magnets, Filtration, Evaporation (and crystallization), Distillation, and Chromatography are separation techniques.
Floatation
- Plastic floats on water and can be scooped out with a net.
Separating Funnel
- Separating funnels are used to separate two immiscible liquids of different densities.
- The less dense liquid floats on top.
- For example, oil floats on water.
- You can separate oil and water by opening the tap and letting the water (the more dense liquid) out into a beaker.
- Use a different beaker to collect the oil.
Centrifuge
- A centrifuge is a machine that creates a rotational force, used to separate a mixture containing particles of different densities.
- The centrifuge forces the more dense particles to the bottom of the tube, forming a pellet.
- The remaining solution on top is called the supernatant.
- The supernatant can be removed off the top with a pipette and placed into a new centrifuge tube.
- The new tube can be spun at a faster speed to further separate the particles remaining in the mixture if they have different densities.
Centrifuging Blood
- If you centrifuge blood, the red blood cells will form a pellet at the bottom of the tube.
Centrifuging Milk
- Centrifuges can be used to separate cream from milk.
- Before the invention of centrifuges, cream was skimmed off the top of milk after it had been sitting for a while.
- Centrifuges make separating cream from milk faster and easier.
Separation Techniques
- Sieving: Separates solids of different sizes
- Decanting: Separates a liquid from a solid by pouring off the liquid carefully. Separating funnel
- Magnets: Separates magnetic solids from non-magnetic materials.
- Filtration: Separates an insoluble solid from a liquid using a filter paper.
- Evaporation: Separates a soluble solid from a liquid by heating the liquid.
- Crystallization: Recrystallized solid is separated from the liquid by heating the solution, evaporation, and cooling.
- Distillation: Separates liquids with different boiling points.
- Chromatography: Separates mixtures based on the different rates at which components move with the mobile phase.
- Floatation: Separates solids based on their density, using a liquid where the solid floats.
- Separating funnel: Separates two immiscible líquidos based on density. The less dense liquid forms a layer on top of the denser liquid.
- Centrifuge: Spins a mixture at high speed, separating components based on their density. Denser particles form a pellet at the bottom, and the less dense liquid forms a supernatant layer.
- Centrifuging applications: Separating blood plasma, cream from milk, and separating mixtures of different densities.
Separating funnel
- Used to separate two immiscible liquids of different densities.
- The less dense liquid floats on top.
- Oil and water are immiscible, oil floats on water.
- To separate, open the tap and let out the lower layer of water (more dense)
- Then collect the oil (less dense).
Centrifuge
- A machine that spins a mixture at high speed.
- Used to separate a mixture containing particles of different densities.
- Denser particles form a pellet at the bottom.
- The remaining solution on top is called the supernatant.
- In centrifugation, the supernatant can be spun at a faster speed to further separate the remaining particles if they have different densities.
- Centrifuging blood: Separates blood components, including red blood cells, white blood cells, and platelets.
- Centrifuging milk: Separates cream (fat) from milk.
- Centrifuging mixtures: Separates different substances based on their densities.
- The mixture is spun, and the largest particle forms a pellet first.
- Each subsequent spin separates the next largest particle at the bottom.
Separating Funnel and Centrifuge Notes
- 1: Describe how to separate oil and water using a separating funnel. Draw a labelled diagram.
- 2: Explain how a centrifuge works to separate mixtures.
- 3: Describe what happens when freshly collected blood is put in a centrifuge and why this happens.
- 4: Describe what happens when freshly collected milk is put in a centrifuge and why this happens.
- 5: Draw a sketch of a centrifuge tube after a mixture has been centrifuged, label the pellet and the supernatant.
Separation Techniques
- Sieving separates mixtures of different sized particles.
- Decanting separates mixtures of a liquid and a solid where the solid settles at the bottom.
- Magnets separate mixtures containing magnetic materials, like iron.
- Filtration separates mixtures of solids and liquids, using filter paper.
- Evaporation separates mixtures of a dissolved solid and a liquid, the liquid evaporates, leaving the solid behind.
- Distillation separates liquids with different boiling points, the liquid with the lower boiling point evaporates first.
- Chromatography separates mixtures based on the different rates at which substances move through a stationary phase.
- Floatation separates mixtures based on the densities of the components.
- Separating Funnel separates immiscible (non-mixing) liquids with different densities, it allows the denser liquid to be drained out.
- Centrifugation separates mixtures based on the densities of the components.
Centrifuges
- Centrifuges rotate at high speeds, causing denser particles to settle at the bottom, creating a pellet.
- The liquid on top is called the supernatant.
- Centrifugation can be used multiple times to separate particles with different densities.
- Freshly collected blood in a centrifuge would separate into red blood cells (pellet), white blood cells and platelets (pellet), and plasma (supernatant).
- Freshly collected milk in a centrifuge would separate into cream (less dense), and skimmed milk (more dense).
- The supernatant from the first spin of a centrifuge can be spun again to further separate the particles remaining in the mixture.
- In a centrifuge tube after a mixture has been centrifuged, the pellet is found at the bottom, and the supernatant is found on top.
Mixtures
- Pure substances are difficult to break down, mixtures are easier.
- Pure substances are elements and compounds
- Mixtures are either homogenous or heterogenous
Homogenous Mixtures
- Particles are spread evenly
- Examples: nitrogen and oxygen in air, steel, vinegar, brass, black coffee, natural gas
Heterogenous Mixtures
- Different components are scattered unevenly
- Examples: salad, chocolate chip cookies, soda with ice.
Types of Mixtures (By particle size)
- Solutions contain very small particles (less than 1000nm)
- Colloids contain particles too small to settle or be removed by filtering
- Suspensions contain larger particles that will settle or be removed by filtering
Solutions
- Appear clear
- Light beam will not be visible when shone through a solution
Colloids
- Appear cloudy
- Light beam will be visible when shone through a colloid
- Examples: milk, mist, cappuccino froth, whipped cream, ice-cream, paint
Suspensions
- Appear cloudy
- Light beam will be visible when shone through a suspension
- Examples: mud mixed with water
Types of Colloids
- Foams - gas trapped within a liquid or solid (e.g. shaving cream)
- Aerosols - liquid or solid particles dispersed in a gas (e.g. fog, smoke)
- Emulsions - two or more insoluble liquids (e.g. homogenized milk, mayonnaise)
- Gels - solid particles in a liquid medium (e.g. gelatin)
- Sols - liquid particles in a solid medium (e.g. ink)
Emulsions
- One liquid is able to spread evenly through another liquid instead of settling
- Unhomogenized milk is not an emulsion, homogenized milk is
- Emulsifiers keep immiscible liquids dispersed
- Examples: mayonnaise, moisturizer, salad dressing
Immiscible Liquids
- Do not mix
- Water and oil are immiscible
- Emulsifiers are chemicals added to immiscible liquids to keep them dispersed
- Lecithin in egg yolk is the emulsifier in mayonnaise
The Tyndall Effect
- The scattering of light by particles present in a colloid or suspension
- The only difference between a colloid and a suspension is the size of the particles
- Colloids have particles between 1-1000 nanometers
- Suspensions have particles from 10-1000 micrometers
Chromatography
- Stationary phase is the paper
- Mobile phase is the water
- Solvent front is the term used to describe how the solvent moves up the stationary phase
- The baseline should be drawn with a pencil below the spots to avoid it dissolving in the solvent
- Dyes that move a long way up the stationary phase have a high solubility in the solvent
- Dyes that do not move very far up the stationary phase have a high affinity for the stationary phase
- The solvent front is drawn at the point where the solvent has travelled furthest
- Chromatograms for two different black ink pens will have different numbers and positions of spots because the dyes in the ink will be different
- Rf value is calculated by the following formula : Rf=distancetravelledbysubstancedistancetravelledbysolventR_f = \frac{distance travelled by substance}{distance travelled by solvent}Rf=distancetravelledbysolventdistancetravelledbysubstance
- The Rf values will change if a different solvent is used
-
Rf values for the spots X, Y and Z are:
- Rf X = 0.86
- Rf Y = 0.67
- Rf Z = 0.53
Decanting
- Decanting is a technique to separate a dense, insoluble solid from a liquid.
- The solid settles at the bottom of the container forming a sediment.
- The liquid is carefully poured into a new container leaving the solid behind.
- Decanting is only useful if the solid particles readily settle to the bottom.
- It is commonly used when pouring some wines after the sediments have settled.
Sieving
- Sieving separates a dry mixture of two solids with different particle sizes.
- A sieve is chosen with holes that allow one particle through but not the other.
- It is used to separate hazelnuts, peppercorns and sugar.
- It is also used to separate pasta from the water it was boiled in.
Filtration
- A special form of sieving that separates very fine solid particles from liquid or gas mixtures.
- Filter paper with fine pores acts as the sieve.
- It separates an insoluble solid from a liquid.
- Sand does not dissolve in water and is considered insoluble.
- When filtering sand and water, the sand (residue) stays behind in the filter paper, while the water (filtrate) passes through.
- Filtration is used to separate insoluble particles that are too large to pass through the filter paper.
Suspensions
- A mixture of very small pieces of solid mixed in a liquid but not dissolved.
- The solid particles will separate from the liquid and either fall to the bottom or rise to the top.
- Examples include sand in water and chalk in water.
- The solid particles will sink to the bottom forming a sediment.
- Suspensions can separate quickly or stay suspended for a longer time depending on their composition.
Solutions
- When something dissolves, it is considered soluble.
- The liquid that dissolves the solid is called the solvent.
- The solid that dissolves is called the solute.
- The new name for the liquid mixture with the dissolved solute is a solution.
- Salt dissolves in water making a salt water solution.
- Salt is the solute and water is the solvent.
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Description
Test your knowledge on various separation techniques used in science, such as filtration, distillation, and chromatography. This quiz covers methods for separating different substances effectively and their applications in real-life scenarios. Challenge yourself to understand the principles behind techniques like centrifugation and the use of separating funnels.