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Questions and Answers
In the lac operon, what happens when lactose is present in the environment?
In the lac operon, what happens when lactose is present in the environment?
- E. coli converts lactose to allolactose, which inactivates the repressor, allowing transcription of the lac operon. (correct)
- The lac operon remains repressed, preventing transcription of lactose-metabolizing genes.
- The repressor protein binds more tightly to the operator sequence, further inhibiting transcription.
- cAMP levels increase, enhancing the binding of the repressor to the operator.
Which of the following is a characteristic of inducible operons?
Which of the following is a characteristic of inducible operons?
- They are typically involved in anabolic pathways.
- They contain genes important for catabolizing certain substances. (correct)
- Their expression is increased when the concentration of the end product is high.
- They are transcribed continually unless deactivated by repressors.
How does cAMP influence the transcription of the lac operon?
How does cAMP influence the transcription of the lac operon?
- cAMP promotes the translation of the repressor protein.
- cAMP increases the rate at which lactose is converted into glucose.
- cAMP directly inhibits the binding of the repressor to the operator.
- cAMP binds to CAP, and the cAMP-CAP complex enhances the transcription of the lac operon. (correct)
What is the role of the operator in the lac operon?
What is the role of the operator in the lac operon?
Which process involves the use of bacteriophages to transfer DNA from one bacterium to another?
Which process involves the use of bacteriophages to transfer DNA from one bacterium to another?
What is the key event in transformation?
What is the key event in transformation?
What is the primary function of error-prone repair mechanisms in DNA repair?
What is the primary function of error-prone repair mechanisms in DNA repair?
Which of the following is an example of a gross mutation?
Which of the following is an example of a gross mutation?
What is the characteristic feature of transposons?
What is the characteristic feature of transposons?
In the trp operon, what happens when tryptophan levels are high?
In the trp operon, what happens when tryptophan levels are high?
Flashcards
Regulation of Genetic Expression
Regulation of Genetic Expression
The nature of prokaryotic operons, consisting of a promoter and a series of genes controlled by a regulatory element called an operator.
Inducible operon
Inducible operon
An operon that can be induced or activated by inducers, important for lactose catabolism.
Repressible operon
Repressible operon
An operon that is transcribed continually until deactivated by repressors.
Point Mutation
Point Mutation
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Transposons
Transposons
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Transformation
Transformation
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Transduction
Transduction
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Tryptophan
Tryptophan
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Mutation
Mutation
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Lac operon
Lac operon
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Study Notes
Scientific Notation
- Used to express very large or very small numbers conveniently
- Expressed as $a \times 10^b$, where $1 \leq |a| < 10$ is a real number and $b$ is an integer
- Example: The speed of light is $3 \times 10^8 m/s$
International System of Units (SI)
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Standard system of units used in science and most countries
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Based on seven base units
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Length: meter (m)
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Mass: kilogram (kg)
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Time: second (s)
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Electrical Current: ampere (A)
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Temperature: kelvin (K)
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Amount of Matter: mole (mol)
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Luminous Intensity: candela (cd)
Derived Units
- Formed by combining base units
- Velocity: $m/s$
- Force: N (newton), where $1 N = 1 kg \cdot m/s^2$
- Energy: J (joule), where $1 J = 1 N \cdot m$
SI Prefixes
-
Used to indicate multiples or submultiples of SI units
-
yotta (Y): $10^{24}$
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zetta (Z): $10^{21}$
-
exa (E): $10^{18}$
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peta (P): $10^{15}$
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tera (T): $10^{12}$
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giga (G): $10^9$
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mega (M): $10^6$
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kilo (k): $10^3$
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hecto (h): $10^2$
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deca (da): $10^1$
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deci (d): $10^{-1}$
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centi (c): $10^{-2}$
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milli (m): $10^{-3}$
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micro ($\mu$): $10^{-6}$
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nano (n): $10^{-9}$
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pico (p): $10^{-12}$
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femto (f): $10^{-15}$
-
atto (a): $10^{-18}$
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zepto (z): $10^{-21}$
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yocto (y): $10^{-24}$
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Example: $1 km = 1000 m = 10^3 m$
Unit Conversion
- Process of converting a measurement from one unit to another using conversion factors
- Example: Converting 5 km to meters ($5 km \times \frac{1000 m}{1 km} = 5000 m$)
Significant Figures
- Digits in a number known with certainty plus one estimated digit
Rules for Determining Significant Figures
- Non-zero digits are significant
- Zeros between non-zero digits are significant
- Leading zeros are not significant
- Trailing zeros are significant only if the number contains a decimal point
Examples of Significant Figures
- 1234 has 4 significant figures
- 1002 has 4 significant figures
- 0.0023 has 2 significant figures
- 1.20 has 3 significant figures
- 1200 has 2 significant figures, but 1200. has 4
Rounding
Rounding Rules
- If the next digit is less than 5, the last significant digit remains the same
- If the next digit is 5 or greater, the last significant digit is increased by 1
Examples of Rounding
- Rounding 3.14159 to 3 significant figures: 3.14
- Rounding 1.666 to 2 significant figures: 1.7
Operations with Significant Figures
- Addition and Subtraction: The result should have the same number of decimal places as the number with the fewest decimal places
- Multiplication and Division: The result should have the same number of significant figures as the number with the fewest significant figures
Examples of Operations with Significant Figures
- $1.234 + 2.3 = 3.5$ (rounded from 3.534)
- $4.56 \times 1.2 = 5.5$ (rounded from 5.472)
Vectors
- Quantity with both magnitude and direction, represented by arrows
Vector Components
- A vector $\vec{A}$ can be decomposed into $A_x$ and $A_y$
- $A_x = A \cos(\theta)$
- $A_y = A \sin(\theta)$
Vector Addition
- Add corresponding components, so if $\vec{C} = \vec{A} + \vec{B}$, then:
- $C_x = A_x + B_x$
- $C_y = A_y + B_y$
- $C = \sqrt{C_x^2 + C_y^2}$
- $\theta = \arctan\left(\frac{C_y}{C_x}\right)$
Dot Product (Scalar Product)
- $\vec{A} \cdot \vec{B} = |\vec{A}| |\vec{B}| \cos(\theta)$
- $\vec{A} \cdot \vec{B} = A_x B_x + A_y B_y + A_z B_z$
Cross Product (Vector Product)
- Results in a vector $\vec{C}$ perpendicular to the plane containing $\vec{A}$ and $\vec{B}$
- $|\vec{C}| = |\vec{A}| |\vec{B}| \sin(\theta)$
- $\vec{C} = \vec{A} \times \vec{B} = (A_y B_z - A_z B_y, A_z B_x - A_x B_z, A_x B_y - A_y B_x)$
Statistics - Chapter 2 - Random Variables
Basic Definitions
- A random variable $X$ is a function that associates a real number with each possible outcome of a random experiment
Types of Random Variables
- Discrete Random Variable: Variable with a countable set of possible values (finite or countably infinite)
- Continuous Random Variable: Variable that can take any value in a given interval
Discrete Random Variables
Definition
- The probability distribution of a discrete RV $X$ is a function assigning a probability to each possible value of $X$
Mass Function
- $f(x) = P(X = x)$
Conditions
- $0 \leq f(x) \leq 1$
- $\sum_{x} f(x) = 1$
Distribution Function
- Indicates the probability that $X$ takes a value less than or equal to $x$
- $F(x) = P(X \leq x) = \sum_{t \leq x} f(t)$
Mathematical Expectation (Expected Value)
- Weighted average of the possible values of $X$
- $E(X) = \mu = \sum_{x} x f(x)$
Variance
- Measures the dispersion of the values of $X$ around its mean
- $Var(X) = \sigma^2 = E[(X - \mu)^2] = \sum_{x} (x - \mu)^2 f(x) = E(X^2) - [E(X)]^2$
Standard Deviation
- The square root of the variance
- $\sigma = \sqrt{Var(X)}$
Continuous Random Variables
Definition
- The probability distribution is described by a probability density function (pdf) $f(x)$
Density function
- $P(a \leq X \leq b) = \int_{a}^{b} f(x) dx$
Conditions
- $f(x) \geq 0$
- $\int_{-\infty}^{\infty} f(x) dx = 1$
Distribution Function
- Indicates the probability that $X$ takes a value less than or equal to $x$
- $F(x) = P(X \leq x) = \int_{-\infty}^{x} f(t) dt$
Mathematical Expectation (Expected Value)
- Average weighted values with weights given by pdf
- $E(X) = \mu = \int_{-\infty}^{\infty} x f(x) dx$
Variance
- Measures the dispersion around the mean
- $Var(X) = \sigma^2 = E[(X - \mu)^2] = \int_{-\infty}^{\infty} (x - \mu)^2 f(x) dx = E(X^2) - [E(X)]^2$
Standard Deviation
- Square root of variance
- $\sigma = \sqrt{Var(X)}$
Common Distributions
Discrete Uniform Law
- All possible values ​​of $X$ have the same probability
- $P(X = x) = \frac{1}{n}$
Bernoulli's Law
- Only has 2 values, 0(failure) and 1(success)
- $P(X = 1) = p$
- $P(X = 0) = 1 - p$
Binomial Law
- Represents the number of successes in n independent Bernoulli trials
- $P(X = k) = \binom{n}{k} p^k (1 - p)^{n - k}$
Poisson's Law
- Represents the number of events that occur in a given interval of time or space
- $P(X = k) = \frac{e^{-\lambda} \lambda^k}{k!}$
Exponential Law
- Represents the waiting time until an event occurs
- $f(x) = \lambda e^{-\lambda x}$
Normal Law
- Symmetrical bell shaped distribution
- $f(x) = \frac{1}{\sigma \sqrt{2\pi}} e^{-\frac{(x - \mu)^2}{2\sigma^2}}$
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