Ruminant Microbiology: Holotrichs and Entodiniomorphs
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Questions and Answers

What is the preferred food source for Holotrichs?

  • Green fodder (correct)
  • High-fiber diet
  • Concentrate ration
  • Mixed ration

On which type of ration do Entodiniomorphs predominate?

  • Green fodder
  • Silage
  • Concentrate ration (correct)
  • Hay

What is the primary difference between the diets of Holotrichs and Entodiniomorphs?

  • Protein content
  • Fiber content
  • Type of food source (correct)
  • Moisture content

Which of the following is NOT a characteristic of Holotrichs?

<p>Ability to break down complex carbohydrates (A)</p> Signup and view all the answers

What can be inferred about the digestive systems of Holotrichs and Entodiniomorphs?

<p>They are specialized for different food sources (C)</p> Signup and view all the answers

What is the significance of studying rumen protozoa?

<p>Developing strategies to improve rumen digestion and reduce greenhouse gas emissions (C)</p> Signup and view all the answers

What is the purpose of gravity-fractionation in this experiment?

<p>To separate protozoa from other microbes in the rumen fluid (D)</p> Signup and view all the answers

What is the temperature at which the rumen fluid was incubated?

<p>39°C (C)</p> Signup and view all the answers

What was the source of the rumen fluid samples in this experiment?

<p>Non-lactating, cannulated Holstein-Friesian cows (A)</p> Signup and view all the answers

How long after feeding were the rumen fluid samples collected?

<p>2 hours (C)</p> Signup and view all the answers

What was done with the remaining rumen fluid after protozoa separation?

<p>It was used for RNA extraction and downstream applications (D)</p> Signup and view all the answers

What is the characteristic feature of Holotrichs cilia?

<p>They have a single posterior cilium that is longer than the others (D)</p> Signup and view all the answers

What role do Holotrichs play in the rumen ecosystem?

<p>They facilitate fungal predation (C)</p> Signup and view all the answers

What type of protozoa are Entodinium and Eudiplodinium classified as?

<p>Entodiniomorphids (C)</p> Signup and view all the answers

What is the purpose of the PCR test in this study?

<p>To confirm the purity of the RNA sample (C)</p> Signup and view all the answers

Which of the following species is NOT a type of Holotrich?

<p>Ostracodinium dilobum (B)</p> Signup and view all the answers

What is the environment in which Holotrichs are typically found?

<p>Rumen of ruminant animals (B)</p> Signup and view all the answers

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