Ruminant Microbiology: Holotrichs and Entodiniomorphs

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What is the preferred food source for Holotrichs?

Green fodder (correct)

High-fiber diet

Concentrate ration

Mixed ration

On which type of ration do Entodiniomorphs predominate?

Green fodder

Silage

Concentrate ration (correct)

Hay

What is the primary difference between the diets of Holotrichs and Entodiniomorphs?

Protein content

Fiber content

Type of food source (correct)

Moisture content

Which of the following is NOT a characteristic of Holotrichs?

Ability to break down complex carbohydrates Signup and view all the answers

What can be inferred about the digestive systems of Holotrichs and Entodiniomorphs?

They are specialized for different food sources Signup and view all the answers

What is the significance of studying rumen protozoa?

Developing strategies to improve rumen digestion and reduce greenhouse gas emissions Signup and view all the answers

What is the purpose of gravity-fractionation in this experiment?

To separate protozoa from other microbes in the rumen fluid Signup and view all the answers

What is the temperature at which the rumen fluid was incubated?

39°C Signup and view all the answers

What was the source of the rumen fluid samples in this experiment?

Non-lactating, cannulated Holstein-Friesian cows Signup and view all the answers

How long after feeding were the rumen fluid samples collected?

2 hours Signup and view all the answers

What was done with the remaining rumen fluid after protozoa separation?

It was used for RNA extraction and downstream applications Signup and view all the answers

What is the characteristic feature of Holotrichs cilia?

They have a single posterior cilium that is longer than the others Signup and view all the answers

What role do Holotrichs play in the rumen ecosystem?

They facilitate fungal predation Signup and view all the answers

What type of protozoa are Entodinium and Eudiplodinium classified as?

Entodiniomorphids Signup and view all the answers

What is the purpose of the PCR test in this study?

To confirm the purity of the RNA sample Signup and view all the answers

Which of the following species is NOT a type of Holotrich?

Ostracodinium dilobum Signup and view all the answers

What is the environment in which Holotrichs are typically found?

Rumen of ruminant animals Signup and view all the answers

Ruminant Microbiology: Holotrichs and Entodiniomorphs

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