Ruminant Microbiology: Holotrichs and Entodiniomorphs
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Questions and Answers

What is the preferred food source for Holotrichs?

  • Green fodder (correct)
  • High-fiber diet
  • Concentrate ration
  • Mixed ration

    • On which type of ration do Entodiniomorphs predominate?

  • Green fodder
  • Silage
  • Concentrate ration (correct)
  • Hay

    • What is the primary difference between the diets of Holotrichs and Entodiniomorphs?

  • Protein content
  • Fiber content
  • Type of food source (correct)
  • Moisture content

    • Which of the following is NOT a characteristic of Holotrichs?

    <p>Ability to break down complex carbohydrates</p> Signup and view all the answers

    What can be inferred about the digestive systems of Holotrichs and Entodiniomorphs?

    <p>They are specialized for different food sources</p> Signup and view all the answers

    What is the significance of studying rumen protozoa?

    <p>Developing strategies to improve rumen digestion and reduce greenhouse gas emissions</p> Signup and view all the answers

    What is the purpose of gravity-fractionation in this experiment?

    <p>To separate protozoa from other microbes in the rumen fluid</p> Signup and view all the answers

    What is the temperature at which the rumen fluid was incubated?

    <p>39°C</p> Signup and view all the answers

    What was the source of the rumen fluid samples in this experiment?

    <p>Non-lactating, cannulated Holstein-Friesian cows</p> Signup and view all the answers

    How long after feeding were the rumen fluid samples collected?

    <p>2 hours</p> Signup and view all the answers

    What was done with the remaining rumen fluid after protozoa separation?

    <p>It was used for RNA extraction and downstream applications</p> Signup and view all the answers

    What is the characteristic feature of Holotrichs cilia?

    <p>They have a single posterior cilium that is longer than the others</p> Signup and view all the answers

    What role do Holotrichs play in the rumen ecosystem?

    <p>They facilitate fungal predation</p> Signup and view all the answers

    What type of protozoa are Entodinium and Eudiplodinium classified as?

    <p>Entodiniomorphids</p> Signup and view all the answers

    What is the purpose of the PCR test in this study?

    <p>To confirm the purity of the RNA sample</p> Signup and view all the answers

    Which of the following species is NOT a type of Holotrich?

    <p>Ostracodinium dilobum</p> Signup and view all the answers

    What is the environment in which Holotrichs are typically found?

    <p>Rumen of ruminant animals</p> Signup and view all the answers

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