Podcast
Questions and Answers
What ensures that each small DNA fragment fixed on the beads can be the unique template for amplification in the sequencing process?
What ensures that each small DNA fragment fixed on the beads can be the unique template for amplification in the sequencing process?
- Use of single-stranded DNA binding protein
- Presence of complementary oligos on the beads (correct)
- Incorporation of PCR reagents in the incubation system
- Combination with magnetic beads after PCR reaction
What is the purpose of using a PTP plate with special nanopores in the pyrosequencing process?
What is the purpose of using a PTP plate with special nanopores in the pyrosequencing process?
- Fix the position of each bead for convenience in sequencing (correct)
- Enhance fluorescence signal detection
- Accelerate the degradation of ATP by diphosphatase
- Accommodate multiple beads in each nanopore
What initiates the release of a pyrophosphate group in the pyrosequencing process?
What initiates the release of a pyrophosphate group in the pyrosequencing process?
- Degradation of ATP by diphosphatase
- Binding of single-stranded DNAs to DNA polymerase
- Combination with magnetic beads after PCR reaction
- Pairing of dNTP with template DNA (correct)
How does the pyrophosphate group produced during DNA synthesis lead to fluorescence in pyrosequencing?
How does the pyrophosphate group produced during DNA synthesis lead to fluorescence in pyrosequencing?
What is the significance of using a CCD camera in the pyrosequencing process?
What is the significance of using a CCD camera in the pyrosequencing process?
Why does 454 sequencing technology conduct each reaction in an independent nanopore on the PTP board?
Why does 454 sequencing technology conduct each reaction in an independent nanopore on the PTP board?
What is the main principle of sequencing in the Roche 454 system?
What is the main principle of sequencing in the Roche 454 system?
What distinguishes the DNA library construction process of Roche 454 from Illumina?
What distinguishes the DNA library construction process of Roche 454 from Illumina?
What is the key technology behind emulsion PCR in the Roche 454 system?
What is the key technology behind emulsion PCR in the Roche 454 system?
How does Roche 454 system ensure that each DNA template is amplified in an independent reaction space?
How does Roche 454 system ensure that each DNA template is amplified in an independent reaction space?
What is the role of mineral oil in the emulsion PCR process of Roche 454 system?
What is the role of mineral oil in the emulsion PCR process of Roche 454 system?
Why is the use of emulsion PCR important in the Roche 454 system?
Why is the use of emulsion PCR important in the Roche 454 system?
