Transcription I
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Questions and Answers

Which complex is responsible for the assembly of a pre-initiation complex with general transcription factors and RNA Polymerase II?

  • TFIIB
  • TFIIA
  • TFIIF
  • TFIID (correct)

Which subunit of TFIID binds directly to the TATA box in the core promoter?

  • TFIIA
  • TFIIB
  • TBP (correct)
  • TAF

Which transcription factor binds to the BRE sequence in the core promoter?

  • TFIIF
  • TFIIA
  • TFIIB (correct)
  • TFIID

Which complex functions as a link between general and specific transcription factors during the initiation step?

<p>Mediator (A)</p> Signup and view all the answers

What is the main function of TFIIH?

<p>Both helicase and protein kinase activity (C)</p> Signup and view all the answers

What is the role of CPSF in the termination step of transcription?

<p>Cleavage of the 3´-end (B)</p> Signup and view all the answers

Which enzyme is responsible for transcription?

<p>RNA Polymerase (B)</p> Signup and view all the answers

What is the direction of RNA synthesis during transcription?

<p>5' to 3' (D)</p> Signup and view all the answers

Which type of polymerase is involved in transcription in prokaryotes?

<p>RNA Polymerase I (C)</p> Signup and view all the answers

What are the protein subunits of prokaryotic RNA Polymerases?

<p>Sigma, Alpha, Beta, Gamma (C)</p> Signup and view all the answers

What is the function of the sigma (s) subunit in prokaryotic transcription?

<p>Initiating transcription (C)</p> Signup and view all the answers

What is the difference between a closed and open promoter complex?

<p>The presence of RNA Polymerase (C)</p> Signup and view all the answers

Which enzyme transcribes rRNA genes in the nucleolus?

<p>RNA Polymerase I (D)</p> Signup and view all the answers

How many rRNA genes are present in a human diploid cell?

<p>400 (D)</p> Signup and view all the answers

What is the core promoter for rRNA genes located?

<p>In the nontranscribed spacer DNA (A)</p> Signup and view all the answers

Which transcription factor binds to the core promoter of rRNA genes to promote assembly of a transcriptionally-active RNA Polymerase I complex?

<p>Selectivity Factor 1 (SL1) (B)</p> Signup and view all the answers

Which enzyme transcribes most genes to synthesize mRNA?

<p>RNA Polymerase II (A)</p> Signup and view all the answers

What is the core promoter of a gene defined as?

<p>The minimal stretch of DNA that directs accurate initiation of transcription (A)</p> Signup and view all the answers

Which RNA Polymerase is responsible for transcribing pre-ribosomal RNA in eukaryotes?

<p>RNA Polymerase I (A)</p> Signup and view all the answers

What is the role of the s subunit in RNA Polymerase holoenzyme?

<p>It determines the specificity of the RNA Polymerase for a given promoter (A)</p> Signup and view all the answers

What is the consensus sequence for the -35 region of the promoter recognized by the s70 subunit in prokaryotes?

<p>TTGACA (B)</p> Signup and view all the answers

What is the function of Gyrase in prokaryotes during transcription?

<p>To create negative supercoils ahead of the transcription bubble (D)</p> Signup and view all the answers

How does template-directed termination occur in prokaryotic transcription?

<p>A G-C rich repeat region followed by a region of A-T base pairs disrupts RNA-DNA association (B)</p> Signup and view all the answers

What are cis-acting elements in transcriptional regulation?

<p>Regulatory DNA sequences specific to each gene (B)</p> Signup and view all the answers

Which type of RNA Polymerase is responsible for the production of ribosomal RNA (rRNA)?

<p>RNA Polymerase I (C)</p> Signup and view all the answers

Which of the following is NOT a component required for eukaryotic RNA Polymerases to initiate transcription of a gene?

<p>Ribonucleotides (D)</p> Signup and view all the answers

What is the main difference between cis-acting elements and trans-acting factors in the regulation of transcription?

<p>Cis-acting elements are DNA sequences near the gene, while trans-acting factors are proteins that bind to those sequences (C)</p> Signup and view all the answers

What are the main types of sequence elements in the core promoter of a gene?

<p>TATA box and initiator element (B)</p> Signup and view all the answers

Which of the following is NOT involved in the initiation step of transcription of all eukaryotic genes?

<p>Mediator complex (C)</p> Signup and view all the answers

What is the role of the C-terminal domain of RNA Polymerase II in the elongation step of transcription?

<p>It regulates the rate of transcription (A)</p> Signup and view all the answers

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