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Questions and Answers

Which complex is responsible for the assembly of a pre-initiation complex with general transcription factors and RNA Polymerase II?

  • TFIIB
  • TFIIA
  • TFIIF
  • TFIID (correct)

    • Which subunit of TFIID binds directly to the TATA box in the core promoter?

  • TFIIA
  • TFIIB
  • TBP (correct)
  • TAF

    • Which transcription factor binds to the BRE sequence in the core promoter?

  • TFIIF
  • TFIIA
  • TFIIB (correct)
  • TFIID

    • Which complex functions as a link between general and specific transcription factors during the initiation step?

    <p>Mediator</p> Signup and view all the answers

    What is the main function of TFIIH?

    <p>Both helicase and protein kinase activity</p> Signup and view all the answers

    What is the role of CPSF in the termination step of transcription?

    <p>Cleavage of the 3´-end</p> Signup and view all the answers

    Which enzyme is responsible for transcription?

    <p>RNA Polymerase</p> Signup and view all the answers

    What is the direction of RNA synthesis during transcription?

    <p>5' to 3'</p> Signup and view all the answers

    Which type of polymerase is involved in transcription in prokaryotes?

    <p>RNA Polymerase I</p> Signup and view all the answers

    What are the protein subunits of prokaryotic RNA Polymerases?

    <p>Sigma, Alpha, Beta, Gamma</p> Signup and view all the answers

    What is the function of the sigma (s) subunit in prokaryotic transcription?

    <p>Initiating transcription</p> Signup and view all the answers

    What is the difference between a closed and open promoter complex?

    <p>The presence of RNA Polymerase</p> Signup and view all the answers

    Which enzyme transcribes rRNA genes in the nucleolus?

    <p>RNA Polymerase I</p> Signup and view all the answers

    How many rRNA genes are present in a human diploid cell?

    <p>400</p> Signup and view all the answers

    What is the core promoter for rRNA genes located?

    <p>In the nontranscribed spacer DNA</p> Signup and view all the answers

    Which transcription factor binds to the core promoter of rRNA genes to promote assembly of a transcriptionally-active RNA Polymerase I complex?

    <p>Selectivity Factor 1 (SL1)</p> Signup and view all the answers

    Which enzyme transcribes most genes to synthesize mRNA?

    <p>RNA Polymerase II</p> Signup and view all the answers

    What is the core promoter of a gene defined as?

    <p>The minimal stretch of DNA that directs accurate initiation of transcription</p> Signup and view all the answers

    Which RNA Polymerase is responsible for transcribing pre-ribosomal RNA in eukaryotes?

    <p>RNA Polymerase I</p> Signup and view all the answers

    What is the role of the s subunit in RNA Polymerase holoenzyme?

    <p>It determines the specificity of the RNA Polymerase for a given promoter</p> Signup and view all the answers

    What is the consensus sequence for the -35 region of the promoter recognized by the s70 subunit in prokaryotes?

    <p>TTGACA</p> Signup and view all the answers

    What is the function of Gyrase in prokaryotes during transcription?

    <p>To create negative supercoils ahead of the transcription bubble</p> Signup and view all the answers

    How does template-directed termination occur in prokaryotic transcription?

    <p>A G-C rich repeat region followed by a region of A-T base pairs disrupts RNA-DNA association</p> Signup and view all the answers

    What are cis-acting elements in transcriptional regulation?

    <p>Regulatory DNA sequences specific to each gene</p> Signup and view all the answers

    Which type of RNA Polymerase is responsible for the production of ribosomal RNA (rRNA)?

    <p>RNA Polymerase I</p> Signup and view all the answers

    Which of the following is NOT a component required for eukaryotic RNA Polymerases to initiate transcription of a gene?

    <p>Ribonucleotides</p> Signup and view all the answers

    What is the main difference between cis-acting elements and trans-acting factors in the regulation of transcription?

    <p>Cis-acting elements are DNA sequences near the gene, while trans-acting factors are proteins that bind to those sequences</p> Signup and view all the answers

    What are the main types of sequence elements in the core promoter of a gene?

    <p>TATA box and initiator element</p> Signup and view all the answers

    Which of the following is NOT involved in the initiation step of transcription of all eukaryotic genes?

    <p>Mediator complex</p> Signup and view all the answers

    What is the role of the C-terminal domain of RNA Polymerase II in the elongation step of transcription?

    <p>It regulates the rate of transcription</p> Signup and view all the answers

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