Podcast
Questions and Answers
What is the characteristic nucleotide sequence of the Pribnow box?
What is the characteristic nucleotide sequence of the Pribnow box?
What is the distance between the TGTTG sequence and the initial base of mRNA?
What is the distance between the TGTTG sequence and the initial base of mRNA?
What happens after RNA polymerase binds to the promoter?
What happens after RNA polymerase binds to the promoter?
Which of the following is a characteristic of RNA polymerase?
Which of the following is a characteristic of RNA polymerase?
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In what direction does RNA polymerase move along the DNA template?
In what direction does RNA polymerase move along the DNA template?
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What is the function of the Pribnow box in transcription
What is the function of the Pribnow box in transcription
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What is the role of the TGTTG sequence in transcription?
What is the role of the TGTTG sequence in transcription?
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Why does RNA polymerase not require a primer to initiate transcription?
Why does RNA polymerase not require a primer to initiate transcription?
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What is the name of the enzyme responsible for RNA polymerase activity?
What is the name of the enzyme responsible for RNA polymerase activity?
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What is the role of the promoter region in transcription?
What is the role of the promoter region in transcription?
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Which of the following is NOT a property of prokaryotic RNA polymerase?
Which of the following is NOT a property of prokaryotic RNA polymerase?
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In the initiation step of transcription, what is the major event that happens?
In the initiation step of transcription, what is the major event that happens?
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What is the difference between the core enzyme and the holoenzyme?
What is the difference between the core enzyme and the holoenzyme?
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What is the role of the σ subunit in prokaryotic transcription?
What is the role of the σ subunit in prokaryotic transcription?
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What is the function of the terminator region in transcription?
What is the function of the terminator region in transcription?
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Which of the following correctly describes the flow of genetic information?
Which of the following correctly describes the flow of genetic information?
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What is the direction of RNA synthesis during elongation?
What is the direction of RNA synthesis during elongation?
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What is the role of the promoter in transcription?
What is the role of the promoter in transcription?
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What is the relationship between the RNA template strand and the synthesized RNA?
What is the relationship between the RNA template strand and the synthesized RNA?
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Which of the following statements is TRUE about the initiation of transcription?
Which of the following statements is TRUE about the initiation of transcription?
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What is the significance of the "start point" in the transcription unit?
What is the significance of the "start point" in the transcription unit?
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What is the primary role of RNA polymerase during transcription?
What is the primary role of RNA polymerase during transcription?
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Which nucleotide pairing occurs during RNA synthesis?
Which nucleotide pairing occurs during RNA synthesis?
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What happens to the DNA strands after RNA transcription is complete?
What happens to the DNA strands after RNA transcription is complete?
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During RNA elongation, in which direction is the RNA transcript synthesized?
During RNA elongation, in which direction is the RNA transcript synthesized?
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What specifies the Base U in RNA during the transcription process?
What specifies the Base U in RNA during the transcription process?
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When does the transcription process stop?
When does the transcription process stop?
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Which base does not pair with deoxyadenosine during RNA synthesis?
Which base does not pair with deoxyadenosine during RNA synthesis?
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What does the 'G' in the DNA template specify during RNA transcription?
What does the 'G' in the DNA template specify during RNA transcription?
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What is the critical role played by small nuclear RNAs (snRNAs) in the splicing of exons?
What is the critical role played by small nuclear RNAs (snRNAs) in the splicing of exons?
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Which of the following features distinguishes eukaryotic transcription from prokaryotic transcription?
Which of the following features distinguishes eukaryotic transcription from prokaryotic transcription?
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What is the primary function of the promoter region in eukaryotic transcription?
What is the primary function of the promoter region in eukaryotic transcription?
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How does mature mRNA exit the nucleus and reach the cytoplasm for translation?
How does mature mRNA exit the nucleus and reach the cytoplasm for translation?
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What is the key difference between eukaryotic and prokaryotic RNA polymerase?
What is the key difference between eukaryotic and prokaryotic RNA polymerase?
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In the context of transcription, what is the function of the promoter region?
In the context of transcription, what is the function of the promoter region?
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What is the role of the ρ-factor (rho) in transcription?
What is the role of the ρ-factor (rho) in transcription?
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In the elongation stage of transcription, what direction does RNA polymerase move along the DNA template?
In the elongation stage of transcription, what direction does RNA polymerase move along the DNA template?
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How does the DNA double helix re-form after transcription has occurred?
How does the DNA double helix re-form after transcription has occurred?
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During transcription, why is the DNA template strand read in a 3' to 5' direction?
During transcription, why is the DNA template strand read in a 3' to 5' direction?
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Transcription, the process of synthesizing RNA from a DNA template, is essential for:
Transcription, the process of synthesizing RNA from a DNA template, is essential for:
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What is the primary difference between the process of transcription and translation?
What is the primary difference between the process of transcription and translation?
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Flashcards
Transcription
Transcription
The process of synthesizing RNA from a DNA template.
RNA polymerase
RNA polymerase
An enzyme that synthesizes RNA by binding to DNA.
Promoter
Promoter
A DNA sequence where RNA polymerase binds to initiate transcription.
5' to 3' direction
5' to 3' direction
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Antiparallel
Antiparallel
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Central Dogma
Central Dogma
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Coding Strand
Coding Strand
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Prokaryotic RNA Polymerase
Prokaryotic RNA Polymerase
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σ Factor
σ Factor
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Transcription Unit
Transcription Unit
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Holoenzyme
Holoenzyme
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Promoter Region
Promoter Region
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Small nuclear RNAs (snRNAs)
Small nuclear RNAs (snRNAs)
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Splicing
Splicing
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Nucleus function in eukaryotes
Nucleus function in eukaryotes
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Differences between prokaryotes and eukaryotes
Differences between prokaryotes and eukaryotes
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Pre-mRNA processing
Pre-mRNA processing
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Pribnow box
Pribnow box
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TGTTG sequence
TGTTG sequence
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Transcription Elongation
Transcription Elongation
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Nucleoside triphosphates
Nucleoside triphosphates
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Local unwinding
Local unwinding
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Pyrophosphate release
Pyrophosphate release
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DNA template strand
DNA template strand
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RNA synthesis direction
RNA synthesis direction
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Base pairing rules
Base pairing rules
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Elongation phase
Elongation phase
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Termination signal
Termination signal
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Reformation of DNA helix
Reformation of DNA helix
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Role of RNA polymerase
Role of RNA polymerase
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Transcription process
Transcription process
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Initiation
Initiation
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Elongation
Elongation
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Template strand of DNA
Template strand of DNA
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Termination
Termination
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ρ-factor (rho)
ρ-factor (rho)
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5ʹ′ and 3ʹ′ ends
5ʹ′ and 3ʹ′ ends
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Study Notes
Biochemistry of Cells - SIJ1003
- Course code: SIJ1003
- Biochemistry of Cell
- Week 12 Lectures
- Date: 31 DEC 2024
Animations
- Transcription animation: https://www.youtube.com/watch?v=ztnfrVHDJbQ
Central Dogma
- Flow of Genetic Information: Replication, Reverse transcription, Transcription to Translation to produce Protein
- DNA to RNA to Protein
Protein Synthesis
- Flow of genetic information: Prokaryotes vs. Eukaryotes differences in protein synthesis
- Prokaryotic cell
- Transcription and Translation are both in the cytoplasm. Translation can begin immediately while transcription is happening.
- Eukaryotic cell
- Transcription takes place in the nucleus.
- Pre-mRNA produced then processed to produce mature mRNA which exits nucleus for translation in the cytoplasm.
Transcription in Prokaryotes
- Transcription unit extends from the promoter to the terminator
- Core enzyme: Made of four peptide subunits (2 α, Ιβ & Ιβ'). Responsible for 5'→3' RNA polymerase activity. Lacks specificity (can not recognize the promoter region on the DNA template).
- σ subunit ('σ factor'): Enables polymerase to recognize the promoter region on DNA.
- Holoenzyme: σ factor plus the core enzyme.
Properties of Prokaryotic RNA Polymerase
- Multi-subunit enzyme
- Recognizes nucleotide sequences (Promoter region) at the beginning of a DNA stretch.
- Makes a complementary RNA copy of DNA template.
- Recognizes the end of the DNA sequence to be transcribed (Terminator region).
Steps in Transcription
- Initiation: RNA polymerase binding to the promoter region on DNA. Characteristic sequence are Pribnow box, and TGTTG sequences.
- Elongation: RNA polymerase begins to synthesize a transcript of a DNA sequence. RNA polymerase does not need a primer, has no known endonuclease or exonuclease activity. Binding to DNA template results in a local unwinding of DNA. Similar to DNA polymerase, it uses nucleoside triphosphates and releases pyrophosphate.
- Termination: RNA polymerase recognizes termination regions on DNA template (p - independent termination).
- p-factor (rho) may be required for release of RNA & RNA polymerase. Termination region exhibits two-fold symmetry owing to the presence of palindromes.
Transcription in Eukaryotes
-
Eukaryotic promoter region
- TATA box or Hogness box: Consensus sequence of nucleotides centered about 25 nucleotides to the left of the initial base, similar to the Pribnow box.
- CAAT box: Consensus sequence of nucleotides centered between 70 and 80 nucleotides to the left of the initial base.
-
Three distinct RNA polymerases:
- RNA polymerase I: synthesizes large ribosomal RNAs.
- RNA polymerase II: synthesizes mRNA precursors.
- RNA polymerase III: produces small RNAs including tRNA and 5s rRNA.
Post-modification to RNA
- 5' capping: First processing reaction for hn RNA. 7-methylguanosine attached through a triphosphate linkage to the 5' end of mRNA. Appears to facilitate initiation of translation & stabilizes mRNA.
- Addition of a poly (A) tail: Present in most eukaryotic mRNAs. A chain of 40–200 adenine nucleotides attached to the 3' end. Added after transcription by poly (A) polymerase. May help stabilize mRNA and facilitate its exit from the nucleus. Tail gradually shortens upon entry into the cytoplasm.
- Removal of introns: Maturation of eukaryotic mRNA involves the removal of RNA sequences (introns). Small nuclear RNAs (Sn RNAs) facilitate intron splicing, resulting in excision of introns and mature mRNA leaves the nucleus through nuclear pores.
Prokaryotes vs. Eukaryotes
- Prokaryotes: No nucleus, transcription & translation occur in the cytoplasm, naked DNA, translation starts immediately while transcription is still occurring.
- Eukaryotes: Transcription occurs in the nucleus, translation in the cytoplasm, pre-mRNA produced then processed to produce mature mRNA, mature mRNA exits the nucleus to the cytoplasm for translation.
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Description
Test your knowledge of RNA polymerase and its functions with this quiz. Explore key concepts such as the Pribnow box, the mRNA synthesis process, and the characteristics of RNA polymerase. Perfect for students studying molecular biology.