RNA Polymerase Characteristics Quiz
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Questions and Answers

What is the characteristic nucleotide sequence of the Pribnow box?

  • TATAAT (correct)
  • TGTTG
  • TTGACA
  • CATATG
  • What is the distance between the TGTTG sequence and the initial base of mRNA?

  • 35 nucleotides (correct)
  • 50 nucleotides
  • 45 nucleotides
  • 10 nucleotides
  • What happens after RNA polymerase binds to the promoter?

  • The DNA strands wind up.
  • RNA polymerase degrades the DNA template.
  • RNA polymerase initiates RNA synthesis at the start point of the template strand. (correct)
  • The RNA polymerase moves to the 3' end of the template strand.
  • Which of the following is a characteristic of RNA polymerase?

    <p>It does not require a primer. (B)</p> Signup and view all the answers

    In what direction does RNA polymerase move along the DNA template?

    <p>3' to 5' (A)</p> Signup and view all the answers

    What is the function of the Pribnow box in transcription

    <p>To provide a binding site for the RNA polymerase. (D)</p> Signup and view all the answers

    What is the role of the TGTTG sequence in transcription?

    <p>To provide a binding site for the RNA polymerase. (D)</p> Signup and view all the answers

    Why does RNA polymerase not require a primer to initiate transcription?

    <p>Because RNA polymerase can synthesize RNA from scratch. (C)</p> Signup and view all the answers

    What is the name of the enzyme responsible for RNA polymerase activity?

    <p>Core enzyme (D)</p> Signup and view all the answers

    What is the role of the promoter region in transcription?

    <p>It is the region where RNA polymerase binds to initiate transcription. (A)</p> Signup and view all the answers

    Which of the following is NOT a property of prokaryotic RNA polymerase?

    <p>It makes a complementary DNA copy of the RNA template. (D)</p> Signup and view all the answers

    In the initiation step of transcription, what is the major event that happens?

    <p>The RNA polymerase binds to the promoter region of the DNA. (B)</p> Signup and view all the answers

    What is the difference between the core enzyme and the holoenzyme?

    <p>The holoenzyme contains the σ subunit, while the core enzyme does not. (D)</p> Signup and view all the answers

    What is the role of the σ subunit in prokaryotic transcription?

    <p>It is responsible for recognizing specific nucleotide sequences (the promoter region) at the beginning of a gene. (B)</p> Signup and view all the answers

    What is the function of the terminator region in transcription?

    <p>It is the site where RNA polymerase detaches from the DNA template. (B)</p> Signup and view all the answers

    Which of the following correctly describes the flow of genetic information?

    <p>DNA → RNA → Protein (C)</p> Signup and view all the answers

    What is the direction of RNA synthesis during elongation?

    <p>From the 5' end to the 3' end (B)</p> Signup and view all the answers

    What is the role of the promoter in transcription?

    <p>To bind to the RNA polymerase and initiate transcription (C)</p> Signup and view all the answers

    What is the relationship between the RNA template strand and the synthesized RNA?

    <p>They are complementary, but with Uracil replacing Thymine in RNA (D)</p> Signup and view all the answers

    Which of the following statements is TRUE about the initiation of transcription?

    <p>The DNA strands unwind to form a transcription bubble (B)</p> Signup and view all the answers

    What is the significance of the "start point" in the transcription unit?

    <p>It is the location where the RNA polymerase begins transcribing the DNA sequence (A)</p> Signup and view all the answers

    What is the primary role of RNA polymerase during transcription?

    <p>To elongate the RNA transcript (B)</p> Signup and view all the answers

    Which nucleotide pairing occurs during RNA synthesis?

    <p>A with U (B)</p> Signup and view all the answers

    What happens to the DNA strands after RNA transcription is complete?

    <p>They re-form a double helix (A)</p> Signup and view all the answers

    During RNA elongation, in which direction is the RNA transcript synthesized?

    <p>5' to 3' (D)</p> Signup and view all the answers

    What specifies the Base U in RNA during the transcription process?

    <p>Adenine from the DNA template (B)</p> Signup and view all the answers

    When does the transcription process stop?

    <p>When a termination signal is reached (A)</p> Signup and view all the answers

    Which base does not pair with deoxyadenosine during RNA synthesis?

    <p>Thymidine (B)</p> Signup and view all the answers

    What does the 'G' in the DNA template specify during RNA transcription?

    <p>C (B)</p> Signup and view all the answers

    What is the critical role played by small nuclear RNAs (snRNAs) in the splicing of exons?

    <p>They bind to the ends of introns, bringing together neighboring exons for splicing. (B)</p> Signup and view all the answers

    Which of the following features distinguishes eukaryotic transcription from prokaryotic transcription?

    <p>Eukaryotic transcription takes place within the nucleus, while translation occurs in the cytoplasm. (D)</p> Signup and view all the answers

    What is the primary function of the promoter region in eukaryotic transcription?

    <p>It provides a binding site for the RNA polymerase, initiating transcription. (D)</p> Signup and view all the answers

    How does mature mRNA exit the nucleus and reach the cytoplasm for translation?

    <p>Through nuclear pores, which act as gates regulating the passage of molecules. (C)</p> Signup and view all the answers

    What is the key difference between eukaryotic and prokaryotic RNA polymerase?

    <p>Eukaryotic RNA polymerase is a single enzyme, while prokaryotic RNA polymerase is comprised of multiple subunits. (B)</p> Signup and view all the answers

    In the context of transcription, what is the function of the promoter region?

    <p>The promoter region binds to RNA polymerase, initiating transcription at the start point. (D)</p> Signup and view all the answers

    What is the role of the ρ-factor (rho) in transcription?

    <p>The ρ-factor (rho) acts as a termination factor, facilitating the separation of RNA polymerase and the newly synthesized RNA transcript. (B)</p> Signup and view all the answers

    In the elongation stage of transcription, what direction does RNA polymerase move along the DNA template?

    <p>5' to 3' (A)</p> Signup and view all the answers

    How does the DNA double helix re-form after transcription has occurred?

    <p>The two strands reunite, forming a double helix, immediately after RNA polymerase has passed a region. (A)</p> Signup and view all the answers

    During transcription, why is the DNA template strand read in a 3' to 5' direction?

    <p>RNA polymerase operates in this direction to ensure the correct sequence of nucleotides is incorporated into the transcript. (C)</p> Signup and view all the answers

    Transcription, the process of synthesizing RNA from a DNA template, is essential for:

    <p>Transporting genetic information from the nucleus to the cytoplasm. (A)</p> Signup and view all the answers

    What is the primary difference between the process of transcription and translation?

    <p>All of the above are true. (E)</p> Signup and view all the answers

    Flashcards

    Transcription

    The process of synthesizing RNA from a DNA template.

    RNA polymerase

    An enzyme that synthesizes RNA by binding to DNA.

    Promoter

    A DNA sequence where RNA polymerase binds to initiate transcription.

    5' to 3' direction

    The direction in which RNA is synthesized during transcription.

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    Antiparallel

    The orientation where RNA runs in the opposite direction to the DNA template strand.

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    Central Dogma

    The flow of genetic information from DNA to RNA to protein.

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    Coding Strand

    The DNA strand that has the same sequence as the RNA produced during transcription (except for T/U).

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    Prokaryotic RNA Polymerase

    An enzyme that synthesizes RNA from a DNA template in prokaryotes.

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    σ Factor

    A protein that enables RNA polymerase to recognize the promoter region of DNA.

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    Transcription Unit

    The segment of DNA that is transcribed into RNA, extending from promoter to terminator.

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    Holoenzyme

    The complete RNA polymerase complex including the core enzyme and the σ factor.

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    Promoter Region

    A specific sequence of DNA where RNA polymerase binds to initiate transcription.

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    Small nuclear RNAs (snRNAs)

    snRNAs facilitate splicing by base pairing with introns to align exons.

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    Splicing

    The process of removing introns and joining exons to form mature mRNA.

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    Nucleus function in eukaryotes

    Eukaryotes transcribe RNA in the nucleus before mRNA exits for translation.

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    Differences between prokaryotes and eukaryotes

    Prokaryotes lack a nucleus; transcription and translation occur simultaneously. Eukaryotes separate these processes.

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    Pre-mRNA processing

    Pre-mRNA undergoes modifications (like splicing) to become mature mRNA.

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    Pribnow box

    A sequence of seven nucleotides centered around ten nucleotides upstream of the mRNA start site.

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    TGTTG sequence

    A sequence located about 35 nucleotides upstream of the mRNA initial base.

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    Transcription Elongation

    The phase where RNA polymerase synthesizes mRNA from the DNA template post-initiation.

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    Nucleoside triphosphates

    The building blocks used by RNA polymerase to add nucleotides to the growing RNA strand.

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    Local unwinding

    The process where the DNA helix unwinds in a localized area during transcription.

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    Pyrophosphate release

    The byproduct released when RNA polymerase adds a nucleotide to the growing RNA chain.

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    DNA template strand

    The strand of DNA that serves as a guide for RNA synthesis.

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    RNA synthesis direction

    RNA is synthesized in the 5' to 3' direction during transcription.

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    Base pairing rules

    In RNA, 'G' pairs with 'C', 'C' pairs with 'G', 'T' pairs with 'A', and 'A' pairs with 'U'.

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    Elongation phase

    The stage of transcription where RNA polymerase elongates the RNA strand.

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    Termination signal

    A specific sequence in DNA that signals the end of RNA synthesis.

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    Reformation of DNA helix

    After transcription, the DNA strands re-form their double helix structure.

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    Role of RNA polymerase

    Enzyme responsible for unwinding DNA and synthesizing RNA during transcription.

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    Transcription process

    The overall process of synthesizing RNA from a DNA template.

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    Initiation

    The process where RNA polymerase binds to the promoter and begins RNA synthesis.

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    Elongation

    The stage in transcription where RNA polymerase adds RNA nucleotides, elongating the RNA strand.

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    Template strand of DNA

    The DNA strand that RNA polymerase uses as a guide to synthesize RNA.

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    Termination

    The end of transcription when RNA polymerase detaches from the DNA and releases RNA.

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    ρ-factor (rho)

    A protein that may play a role in terminating transcription by helping release RNA and RNA polymerase.

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    5ʹ′ and 3ʹ′ ends

    Refers to the orientation of nucleotides in RNA and DNA; crucial for understanding transcription direction.

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    Study Notes

    Biochemistry of Cells - SIJ1003

    • Course code: SIJ1003
    • Biochemistry of Cell
    • Week 12 Lectures
    • Date: 31 DEC 2024

    Animations

    Central Dogma

    • Flow of Genetic Information: Replication, Reverse transcription, Transcription to Translation to produce Protein
    • DNA to RNA to Protein

    Protein Synthesis

    • Flow of genetic information: Prokaryotes vs. Eukaryotes differences in protein synthesis
    • Prokaryotic cell
      • Transcription and Translation are both in the cytoplasm. Translation can begin immediately while transcription is happening.
    • Eukaryotic cell
      • Transcription takes place in the nucleus.
      • Pre-mRNA produced then processed to produce mature mRNA which exits nucleus for translation in the cytoplasm.

    Transcription in Prokaryotes

    • Transcription unit extends from the promoter to the terminator
    • Core enzyme: Made of four peptide subunits (2 α, Ιβ & Ιβ'). Responsible for 5'→3' RNA polymerase activity. Lacks specificity (can not recognize the promoter region on the DNA template).
    • ‎σ subunit ('σ factor'): Enables polymerase to recognize the promoter region on DNA.
    • Holoenzyme: ‎σ factor plus the core enzyme.

    Properties of Prokaryotic RNA Polymerase

    • Multi-subunit enzyme
    • Recognizes nucleotide sequences (Promoter region) at the beginning of a DNA stretch.
    • Makes a complementary RNA copy of DNA template.
    • Recognizes the end of the DNA sequence to be transcribed (Terminator region).

    Steps in Transcription

    • Initiation: RNA polymerase binding to the promoter region on DNA. Characteristic sequence are Pribnow box, and TGTTG sequences.
    • Elongation: RNA polymerase begins to synthesize a transcript of a DNA sequence. RNA polymerase does not need a primer, has no known endonuclease or exonuclease activity. Binding to DNA template results in a local unwinding of DNA. Similar to DNA polymerase, it uses nucleoside triphosphates and releases pyrophosphate.
    • Termination: RNA polymerase recognizes termination regions on DNA template (p - independent termination).
    • p-factor (rho) may be required for release of RNA & RNA polymerase. Termination region exhibits two-fold symmetry owing to the presence of palindromes.

    Transcription in Eukaryotes

    • Eukaryotic promoter region

      • TATA box or Hogness box: Consensus sequence of nucleotides centered about 25 nucleotides to the left of the initial base, similar to the Pribnow box.
      • CAAT box: Consensus sequence of nucleotides centered between 70 and 80 nucleotides to the left of the initial base.
    • Three distinct RNA polymerases:

      • RNA polymerase I: synthesizes large ribosomal RNAs.
      • RNA polymerase II: synthesizes mRNA precursors.
      • RNA polymerase III: produces small RNAs including tRNA and 5s rRNA.

    Post-modification to RNA

    • 5' capping: First processing reaction for hn RNA. 7-methylguanosine attached through a triphosphate linkage to the 5' end of mRNA. Appears to facilitate initiation of translation & stabilizes mRNA.
    • Addition of a poly (A) tail: Present in most eukaryotic mRNAs. A chain of 40–200 adenine nucleotides attached to the 3' end. Added after transcription by poly (A) polymerase. May help stabilize mRNA and facilitate its exit from the nucleus. Tail gradually shortens upon entry into the cytoplasm.
    • Removal of introns: Maturation of eukaryotic mRNA involves the removal of RNA sequences (introns). Small nuclear RNAs (Sn RNAs) facilitate intron splicing, resulting in excision of introns and mature mRNA leaves the nucleus through nuclear pores.

    Prokaryotes vs. Eukaryotes

    • Prokaryotes: No nucleus, transcription & translation occur in the cytoplasm, naked DNA, translation starts immediately while transcription is still occurring.
    • Eukaryotes: Transcription occurs in the nucleus, translation in the cytoplasm, pre-mRNA produced then processed to produce mature mRNA, mature mRNA exits the nucleus to the cytoplasm for translation.

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    Test your knowledge of RNA polymerase and its functions with this quiz. Explore key concepts such as the Pribnow box, the mRNA synthesis process, and the characteristics of RNA polymerase. Perfect for students studying molecular biology.

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