RNA Extraction Procedure Quiz

Nucleic Acid Isolation and Testing

• Boiling the nucleic acid sample with 10% H2SO4 for 1-2 minutes is done to hydrolyze the sample.
• Adding ammonia to the hydrolyzed sample before testing for purines is done to convert the purines to their corresponding amines.
• Adding ammonium molybdate reagent to the hydrolyzed sample is done to detect the presence of inorganic phosphate in nucleic acids.
• RNA is generally more abundant in tissues than DNA.
• Washing the residue twice with 5 mL of 95% ethanol is done to remove impurities and excess water.
• The alpha-naphthol test is performed on the hydrolyzed sample to detect the presence of purines in nucleic acids.
• The alpha-naphthol test is used to detect the presence of purines in nucleic acids.
• The ammonium molybdate test is used to detect the presence of inorganic phosphate in nucleic acids.
• RNA is generally more abundant in actively dividing cells compared to non-dividing cells.
• Washing the residue with ethanol during the isolation of nucleic acids is done to remove impurities and excess water.
• Adding dilute HCl drop by drop until the solution is acidic in the isolation of yeast RNA is done to precipitate RNA.
• The biuret test is performed by adding 1 ml of 10% NaOH and 5 drops CuSO4 to a small amount of nucleic acid and is used to test for the presence of peptide bonds.
• When different portions of a nucleic acid solution are tested with litmus, alizarin, and congo red solution, it indicates the presence of acidic and basic groups.
• Washing the residue with ethanol in the isolation of yeast RNA is important to remove impurities and excess water.
• The biuret test is used to test for the presence of peptide bonds.
• Coagulation occurs upon boiling when testing the solubility of RNA in hot water because RNA is insoluble in hot water.