RNA Extraction Procedure Quiz

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Questions and Answers

What is the purpose of boiling the nucleic acid sample with 10% H2SO4 for 1-2 minutes?

To denature the nucleic acid and make it more soluble

To hydrolyze the nucleic acid and liberate its components (correct)

To concentrate the nucleic acid in the sample

To extract the nucleic acid from the sample

What is the purpose of adding ammonia to the hydrolyzed sample before testing for purines?

To precipitate out the purines

To increase the solubility of the purines

To convert the purines to their ammonium salts (correct)

To neutralize the acidic sample

What is the purpose of adding ammonium molybdate reagent to the hydrolyzed sample?

To test for the presence of purines

To test for the presence of proteins

To test for the presence of phosphates (correct)

To test for the presence of sugars

Based on the information provided, which of the following statements is true regarding the abundance of RNA and DNA in tissues?

The relative abundance of RNA and DNA varies across different tissues Signup and view all the answers

What is the purpose of washing the residue twice with 5 mL of 95% ethanol?

To remove any impurities or contaminants from the RNA Signup and view all the answers

What is the purpose of the alpha-naphthol test performed on the hydrolyzed sample?

To test for the presence of carbohydrates (sugars) Signup and view all the answers

Which test is used to detect the presence of inorganic phosphate in nucleic acids?

Ammonium molybdate test Signup and view all the answers

What is the purpose of the alpha-naphthol test in the context of nucleic acid isolation?

To detect the presence of pentose sugars Signup and view all the answers

Which test is commonly used to detect the presence of purines in nucleic acids?

Murexide test Signup and view all the answers

Which type of nucleic acid is generally more abundant in actively dividing cells compared to non-dividing cells?

RNA Signup and view all the answers

What is the purpose of washing the residue with ethanol during the isolation of nucleic acids?

To remove lipids and other hydrophobic contaminants Signup and view all the answers

Which step in the provided procedure involves the use of acid to precipitate RNA?

Step 4 (adding glacial acetic acid) Signup and view all the answers

What is the purpose of adding dilute HCl drop by drop until the solution is acidic in the isolation of yeast RNA?

To check the solubility of RNA in acidic conditions Signup and view all the answers

Which test is performed by adding 1 ml of 10% NaOH and 5 drops CuSO4 to a small amount of nucleic acid?

Test for purines Signup and view all the answers

What happens when different portions of a nucleic acid solution are tested with litmus, alizarin, and congo red solution?

They change color based on the pH of the solution Signup and view all the answers

Why is it important to wash the residue with ethanol in the isolation of yeast RNA?

To remove impurities from the residue Signup and view all the answers

What is tested by applying the biuret test to a small amount of nucleic acid?

Presence of proteins Signup and view all the answers

Why does coagulation occur upon boiling when testing the solubility of RNA in hot water?

RNA denatures under heat Signup and view all the answers

Study Notes

Nucleic Acid Isolation and Testing

Boiling the nucleic acid sample with 10% H2SO4 for 1-2 minutes is done to hydrolyze the sample.
Adding ammonia to the hydrolyzed sample before testing for purines is done to convert the purines to their corresponding amines.
Adding ammonium molybdate reagent to the hydrolyzed sample is done to detect the presence of inorganic phosphate in nucleic acids.
RNA is generally more abundant in tissues than DNA.
Washing the residue twice with 5 mL of 95% ethanol is done to remove impurities and excess water.
The alpha-naphthol test is performed on the hydrolyzed sample to detect the presence of purines in nucleic acids.
The alpha-naphthol test is used to detect the presence of purines in nucleic acids.
The ammonium molybdate test is used to detect the presence of inorganic phosphate in nucleic acids.
RNA is generally more abundant in actively dividing cells compared to non-dividing cells.
Washing the residue with ethanol during the isolation of nucleic acids is done to remove impurities and excess water.
Adding dilute HCl drop by drop until the solution is acidic in the isolation of yeast RNA is done to precipitate RNA.
The biuret test is performed by adding 1 ml of 10% NaOH and 5 drops CuSO4 to a small amount of nucleic acid and is used to test for the presence of peptide bonds.
When different portions of a nucleic acid solution are tested with litmus, alizarin, and congo red solution, it indicates the presence of acidic and basic groups.
Washing the residue with ethanol in the isolation of yeast RNA is important to remove impurities and excess water.
The biuret test is used to test for the presence of peptide bonds.
Coagulation occurs upon boiling when testing the solubility of RNA in hot water because RNA is insoluble in hot water.

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Description

Test your knowledge on the RNA extraction procedure. Questions cover steps like mixing RNA with ethanol and HCl, settling, decanting, washing with ethanol, and calculating the percentage of RNA obtained. The quiz also includes information on the solubility of RNA in cold water and its differences from DNA.