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Questions and Answers
What is the approximate percentage of mRNA in a mammalian cell's total RNA?
What is the approximate percentage of mRNA in a mammalian cell's total RNA?
- 5-10%
- 15-20%
- 1-5% (correct)
- 80-85%
What serves as a proxy for RNA integrity in terms of the 28S and 18S rRNA ratio?
What serves as a proxy for RNA integrity in terms of the 28S and 18S rRNA ratio?
- $18S/28S$ ratio
- $28S + 18S$
- $28S - 18S$
- $28S/18S$ ratio (correct)
Which rRNA species is more susceptible to degradation?
Which rRNA species is more susceptible to degradation?
- $28S$ rRNA (correct)
- $5.8S$ rRNA
- $5S$ rRNA
- $18S$ rRNA
What is the size ratio of 28S to 18S rRNA in mouse species?
What is the size ratio of 28S to 18S rRNA in mouse species?
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What are the molar amounts of 18S and 28S rRNA molecules in cells?
What are the molar amounts of 18S and 28S rRNA molecules in cells?
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Study Notes
RNA Composition and Integrity
- Approximately 2-5% of a mammalian cell's total RNA is mRNA.
- The 28S and 18S rRNA ratio serves as a proxy for RNA integrity.
- The 18S rRNA species is more susceptible to degradation.
rRNA Size and Abundance
- In mouse species, the 28S rRNA is approximately twice the size of the 18S rRNA.
- There are approximately 4-6 times more 18S rRNA molecules than 28S rRNA molecules in cells, on a molar basis.
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