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Questions and Answers
What is the approximate percentage of mRNA in a mammalian cell's total RNA?
What is the approximate percentage of mRNA in a mammalian cell's total RNA?
What serves as a proxy for RNA integrity in terms of the 28S and 18S rRNA ratio?
What serves as a proxy for RNA integrity in terms of the 28S and 18S rRNA ratio?
Which rRNA species is more susceptible to degradation?
Which rRNA species is more susceptible to degradation?
What is the size ratio of 28S to 18S rRNA in mouse species?
What is the size ratio of 28S to 18S rRNA in mouse species?
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What are the molar amounts of 18S and 28S rRNA molecules in cells?
What are the molar amounts of 18S and 28S rRNA molecules in cells?
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Study Notes
RNA Composition and Integrity
- Approximately 2-5% of a mammalian cell's total RNA is mRNA.
- The 28S and 18S rRNA ratio serves as a proxy for RNA integrity.
- The 18S rRNA species is more susceptible to degradation.
rRNA Size and Abundance
- In mouse species, the 28S rRNA is approximately twice the size of the 18S rRNA.
- There are approximately 4-6 times more 18S rRNA molecules than 28S rRNA molecules in cells, on a molar basis.
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Description
Test your knowledge on the process of isolating total RNA from mouse tissue, determining its quantity and quality, and synthesizing cDNA using the reverse transcription reaction.
