5 Questions
What is the approximate percentage of mRNA in a mammalian cell's total RNA?
1-5%
What serves as a proxy for RNA integrity in terms of the 28S and 18S rRNA ratio?
$28S/18S$ ratio
Which rRNA species is more susceptible to degradation?
$28S$ rRNA
What is the size ratio of 28S to 18S rRNA in mouse species?
$2.53$
What are the molar amounts of 18S and 28S rRNA molecules in cells?
Equal molar amounts
Test your knowledge on the process of isolating total RNA from mouse tissue, determining its quantity and quality, and synthesizing cDNA using the reverse transcription reaction.
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