Podcast
Questions and Answers
What is the function of the 7-methylguanosine cap at the 5’end of eukaryotic mRNAs?
What is the function of the 7-methylguanosine cap at the 5’end of eukaryotic mRNAs?
What is the role of snRNAs in splicing mechanisms?
What is the role of snRNAs in splicing mechanisms?
How can alternative splicing be regulated?
How can alternative splicing be regulated?
What is the physiological importance of alternative polyadenylation site selection?
What is the physiological importance of alternative polyadenylation site selection?
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What is the purpose of 5' cap formation in eukaryotic mRNAs?
What is the purpose of 5' cap formation in eukaryotic mRNAs?
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Which molecule achieves specificity through base-pairing with pre-mRNA splice sites during splicing?
Which molecule achieves specificity through base-pairing with pre-mRNA splice sites during splicing?
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What is the function of the spliceosome in pre-mRNA splicing?
What is the function of the spliceosome in pre-mRNA splicing?
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Which type of gene processing generates multiple mRNA isoforms from a single gene?
Which type of gene processing generates multiple mRNA isoforms from a single gene?
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What is the main role of the carboxy-terminal domain (CTD) of RNA polymerase II in recruiting RNA processing factors?
What is the main role of the carboxy-terminal domain (CTD) of RNA polymerase II in recruiting RNA processing factors?
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How are bacterial and eukaryotic mRNA molecules different in structure?
How are bacterial and eukaryotic mRNA molecules different in structure?
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What is the process of removing introns from the pre-mRNA called?
What is the process of removing introns from the pre-mRNA called?
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'Drosophila's DSCAM gene can potentially produce over how many isoforms through alternative splicing?
'Drosophila's DSCAM gene can potentially produce over how many isoforms through alternative splicing?
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Which of the following is NOT a function of the poly(A) tail in eukaryotes?
Which of the following is NOT a function of the poly(A) tail in eukaryotes?
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Intron retention due to missplicing can lead to which of the following human diseases?
Intron retention due to missplicing can lead to which of the following human diseases?
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Which protein complex is involved in the cleavage and polyadenylation at the 3’ end of eukaryotic pre-mRNAs?
Which protein complex is involved in the cleavage and polyadenylation at the 3’ end of eukaryotic pre-mRNAs?
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What is the primary role of mRNP exporters in eukaryotic cells?
What is the primary role of mRNP exporters in eukaryotic cells?
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Which genetic mutation can lead to ß-thalassemia, an inherited blood disorder?
Which genetic mutation can lead to ß-thalassemia, an inherited blood disorder?
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What can lead to different transcript variants in certain tissues/cells in eukaryotes?
What can lead to different transcript variants in certain tissues/cells in eukaryotes?
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Which factor is responsible for binding to the AAUAAA sequence during cleavage and polyadenylation at the 3’ end of eukaryotic pre-mRNAs?
Which factor is responsible for binding to the AAUAAA sequence during cleavage and polyadenylation at the 3’ end of eukaryotic pre-mRNAs?
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What kind of mutations can cause Myotonic dystrophy, a neuromuscular disease?
What kind of mutations can cause Myotonic dystrophy, a neuromuscular disease?
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Which process involves capture of polyadenylated RNAs (mRNAs) by hybridization to oligo-(dT) coupled magnetic beads?
Which process involves capture of polyadenylated RNAs (mRNAs) by hybridization to oligo-(dT) coupled magnetic beads?
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Which modification occurs to residue 1 during addition of the 5’ cap to mRNA in eukaryotic cells?
Which modification occurs to residue 1 during addition of the 5’ cap to mRNA in eukaryotic cells?
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Study Notes
- Today's lecture topic: Structure and processing of mRNA molecules in eukaryotes and prokaryotes
- Bacterial and eukaryotic mRNA molecules differ in structure: operon, 5' UTR, 3'UTR
- Pre-mRNA processing starts cotranscriptionally
- RNA processing factors are recruited by the phosphorylated carboxy-terminal domain (CTD) of RNA polymerase II
- Eukaryotic mRNAs undergo 5'cap formation, which marks the RNA as mRNA, protects it from degradation, and promotes translation
- Splicing is the process of removing introns from the pre-mRNA
- Introns are much longer than exons, and the splicing machinery achieves specificity through base-pairing between non-coding RNAs (snRNAs) and the pre-mRNA splice sites
- The spliceosome, a large complex of snRNAs and proteins, mediates pre-mRNA splicing
- Alternative splicing generates multiple mRNA isoforms from a single gene, leading to protein diversity
- Drosophila's DSCAM gene is the most extreme example of alternative splicing, with the potential to produce over 38,000 isoforms.
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Description
Test your knowledge on RNA biology and posttranscriptional gene regulation with this quiz. Includes topics such as RNA processing in the nucleus, life and death of mRNAs in the cytoplasm, and non-coding RNA.
