Rickettsia, Chlamydia, and Mycoplasma Classification
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Questions and Answers

Which of the following is NOT a medically important genus in the Family Rickettsiaceae?

  • Mycoplasma (correct)
  • Coxiella
  • Rickettsia
  • Rochlimaea
  • How are Rochlimaea cultivated?

  • In tissue culture
  • On artificial media containing blood (correct)
  • In embryonated eggs
  • By direct detection in tissues
  • Which staining method is used to readily stain Rickettsia?

  • Gram stain
  • Acid-fast stain
  • Ziehl-Neelsen stain
  • Giemsa stain (correct)
  • What is a better serological diagnostic test for Rickettsial antigens?

    <p>Complement fixation tests</p> Signup and view all the answers

    Which virulence factor is associated with Coxiella burnetti?

    <p>Resistance to lysosomal enzymes</p> Signup and view all the answers

    Study Notes

    Rickettsiaceae Family

    • Rickettsiaceae includes several medically important genera such as Rickettsia and Orientia.
    • An example of a genus not considered medically important in this family could be Rochlimaea or another lesser-known genus.

    Cultivation of Rochlimaea

    • Rochlimaea can be cultivated in specialized conditions, such as in yolk sacs of embryonated eggs or in tissue culture.
    • Requires specific growth media to support the survival and reproduction of the organism.

    Staining Methods for Rickettsia

    • The Giemsa staining method is often used to stain Rickettsia, making them readily visible under a microscope.
    • Staining helps differentiate Rickettsia and visualize their morphology.

    Serological Testing for Rickettsial Antigens

    • Indirect immunofluorescence assays (IFA) are considered more effective for serological diagnosis of Rickettsial infections.
    • IFA detects specific antibodies against Rickettsial antigens in patient serum.

    Virulence Factor of Coxiella burnetti

    • The virulence factor associated with Coxiella burnetti is its ability to survive and replicate in phagolysosomes of host cells.
    • This capability aids in its pathogenicity and contributes to Q fever infections.

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    Description

    Test your knowledge on the classification, morphology, and cultural characteristics of Rickettsia, Chlamydia, and Mycoplasma. Learn about the medically important genera within the family Rickettsiaceae and their growth requirements.

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