Podcast
Questions and Answers
What are the potential roles of Streptomyces in plant development and protection?
What are the potential roles of Streptomyces in plant development and protection?
Streptomyces can potentially increase plant growth and protect plants against various pathogens.
Which isolates showed the highest percentage of disease suppression effectiveness against bacterial leaf blight?
Which isolates showed the highest percentage of disease suppression effectiveness against bacterial leaf blight?
Act-SK2, Act-Mn2, Act-Hr21, and Act-Pha4
What are the potential biocontrol agents identified in the study?
What are the potential biocontrol agents identified in the study?
Streptomyces sp. and Penicillium janthinellum
What are the reported qualities contributing to Streptomyces' ability to combat plant pathogens?
What are the reported qualities contributing to Streptomyces' ability to combat plant pathogens?
What is the main role of Penicillium janthinellum in agriculture?
What is the main role of Penicillium janthinellum in agriculture?
What is the main disease of rice mentioned in the text?
What is the main disease of rice mentioned in the text?
What are the symptoms of BLB disease?
What are the symptoms of BLB disease?
What are the potential yield losses in tropical Asia due to BLB disease?
What are the potential yield losses in tropical Asia due to BLB disease?
In which country does the text mention a yield loss of 70-80% due to BLB disease?
In which country does the text mention a yield loss of 70-80% due to BLB disease?
What medium was used to culture the Xoo strain?
What medium was used to culture the Xoo strain?
How was the effectiveness of suppressing incubation period calculated?
How was the effectiveness of suppressing incubation period calculated?
What method was used for Xoo inoculation?
What method was used for Xoo inoculation?
What formula was used to calculate disease severity?
What formula was used to calculate disease severity?
What is the significance of Xoo bacteria in relation to bacterial leaf blight (BLB)?
What is the significance of Xoo bacteria in relation to bacterial leaf blight (BLB)?
How can BLB be controlled using bactericides, and what are the potential drawbacks of continuous use?
How can BLB be controlled using bactericides, and what are the potential drawbacks of continuous use?
What is the role of PGPR and PGPF in biological control of plant diseases?
What is the role of PGPR and PGPF in biological control of plant diseases?
What are some examples of beneficial microbes studied for their ability to suppress Xoo bacteria?
What are some examples of beneficial microbes studied for their ability to suppress Xoo bacteria?
What technique was used to amplify Gene 16S rRNA of isolates Act Pha4, Act LB3, Act Sk2, and Act Hr21?
What technique was used to amplify Gene 16S rRNA of isolates Act Pha4, Act LB3, Act Sk2, and Act Hr21?
How was the phylogenetic analysis conducted?
How was the phylogenetic analysis conducted?
What test was used to confirm that beneficial microbes were not plant pathogenic?
What test was used to confirm that beneficial microbes were not plant pathogenic?
What was the range of the incubation periods of BLB disease in rice plants treated with beneficial microbes?
What was the range of the incubation periods of BLB disease in rice plants treated with beneficial microbes?
What does the AUDPC value indicate in the context of rice plants and beneficial microbe isolates?
What does the AUDPC value indicate in the context of rice plants and beneficial microbe isolates?
How do beneficial microbes influence host plant growth?
How do beneficial microbes influence host plant growth?
What are the commonly used molecular markers for bacterial and fungal classification?
What are the commonly used molecular markers for bacterial and fungal classification?
Which actinobacteria isolates were phylogenetically divided into two groups?
Which actinobacteria isolates were phylogenetically divided into two groups?
Study Notes
- The AUDPC (Area Under Disease Progress Curve) number indicates the resistance level of rice plants after beneficial microbes isolates application.
- Four beneficial microbes isolates (Act-Ak2, Act-Mn2, Act-Hr21, and Act-Pha4) have the lowest AUDPC values, ranging between 51-82.33, suppressing bacterial leaf blight (BLB) development.
- The AUDPC value illustrates the disease development rate over time in each beneficial microbes isolate treatment.
- AUDPC is considered a genotype's resistance level, with low values indicating resistance and high values indicating susceptibility.
- The development of BLB symptoms can be seen in Figure 1, with fifteen beneficial microbes isolates suppressing the growth of BLB disease compared to the control.
- Beneficial microbes have direct and indirect mechanisms to influence host plant growth, including producing essential growth factors and inducing plant resistance through disease suppression and antimicrobial secondary metabolites.
- The obtained sequence length of beneficial microbes isolates ranges from 1157-1535 bp.
- Act-Hr21, Act-Pha4, and Act Sk2 have high sequence similarities with known Streptomyces strains (MK675528, MK811436, and AB373961, respectively).
- ActMn2 was identified using a genetic marker based on the ITS region and is similar to Penicillium janthinellum strain CMV006C1.
- The AUDPC, ITS region, and 16S rRNA genes are commonly used molecular markers for bacterial and fungal classification, respectively.
- Streptomyces is a well-known actinobacteria genus, and the three actinobacteria isolates were phylogenetically divided into two groups.
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Description
Learn about the scale and severity of Bacterial Leaf Blight (BLB) disease in rice plants, as well as the PCR amplification technique used to study isolates Act Pha4, Act LB3, Act Sk2, and Act Hr21. Dive into the Standard Evaluation System for Rice (SES) and PCR amplification of Gene 16S rRNA.
