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Questions and Answers
What will happen to students found cheating in the biology lab?
What will happen to students found cheating in the biology lab?
During which hours are the biology labs open on weekdays?
During which hours are the biology labs open on weekdays?
What should a student do when in doubt about lab procedures?
What should a student do when in doubt about lab procedures?
What materials are listed for use in the first lab exercise on terminology?
What materials are listed for use in the first lab exercise on terminology?
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What can happen to students who fail to adhere to the lab policies?
What can happen to students who fail to adhere to the lab policies?
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What does the term 'medial' refer to in anatomical terminology?
What does the term 'medial' refer to in anatomical terminology?
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Which of the following terms describes the position 'below' in anatomical terminology?
Which of the following terms describes the position 'below' in anatomical terminology?
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In a four-legged animal, which terms would correctly describe positions analogous to 'anterior' and 'posterior' in humans?
In a four-legged animal, which terms would correctly describe positions analogous to 'anterior' and 'posterior' in humans?
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What is the anatomical term for the 'elbow' region?
What is the anatomical term for the 'elbow' region?
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Which term describes the 'spinal side' of the body?
Which term describes the 'spinal side' of the body?
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What does 'proximal' indicate in anatomical terminology?
What does 'proximal' indicate in anatomical terminology?
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Which of the following anatomical terms refers to the 'thigh' region?
Which of the following anatomical terms refers to the 'thigh' region?
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Which anatomical term refers to the 'palm' of the hand?
Which anatomical term refers to the 'palm' of the hand?
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What should NEVER be used at high power to prevent damage to the specimen and the objective lens?
What should NEVER be used at high power to prevent damage to the specimen and the objective lens?
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What is the purpose of the revolving nosepiece in a compound light microscope?
What is the purpose of the revolving nosepiece in a compound light microscope?
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How is total magnification calculated for a microscope with an eyepiece power of 10x?
How is total magnification calculated for a microscope with an eyepiece power of 10x?
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Which part of the microscope is responsible for focusing light on the specimen?
Which part of the microscope is responsible for focusing light on the specimen?
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What is the function of the iris diaphragm ring in a compound light microscope?
What is the function of the iris diaphragm ring in a compound light microscope?
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What is the working distance in the context of a microscope?
What is the working distance in the context of a microscope?
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What should be used exclusively to clean the lenses of a microscope?
What should be used exclusively to clean the lenses of a microscope?
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Which of the following is NOT a function of the stage in a compound light microscope?
Which of the following is NOT a function of the stage in a compound light microscope?
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Which term refers to the viewing area seen when looking through the ocular lenses?
Which term refers to the viewing area seen when looking through the ocular lenses?
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What is the primary risk of moving the specimen without observing any movement through the eyepiece?
What is the primary risk of moving the specimen without observing any movement through the eyepiece?
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What is the recommended procedure when starting to focus on a specimen?
What is the recommended procedure when starting to focus on a specimen?
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Why is it advisable to use the lowest magnification necessary for observations?
Why is it advisable to use the lowest magnification necessary for observations?
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What does the term parfocal mean in the context of a microscope?
What does the term parfocal mean in the context of a microscope?
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What should be done before increasing magnification to avoid losing focus?
What should be done before increasing magnification to avoid losing focus?
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Which mechanism is primarily used to adjust light intensity?
Which mechanism is primarily used to adjust light intensity?
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What can be used to enhance detail in a specimen under high magnification?
What can be used to enhance detail in a specimen under high magnification?
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What is the first step you should take when preparing to use the microscope?
What is the first step you should take when preparing to use the microscope?
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Which focus knob should be used at high power magnification?
Which focus knob should be used at high power magnification?
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What should be done to the stage before stowing the microscope?
What should be done to the stage before stowing the microscope?
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What is the general rule regarding the use of magnification when looking at specimens?
What is the general rule regarding the use of magnification when looking at specimens?
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Which of the following should be avoided when adjusting the light level during microscopy?
Which of the following should be avoided when adjusting the light level during microscopy?
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When should the dust cover be placed over the microscope?
When should the dust cover be placed over the microscope?
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Which method is correct for unplugging the microscope's power cord?
Which method is correct for unplugging the microscope's power cord?
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What is a proper way to carry the microscope?
What is a proper way to carry the microscope?
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Study Notes
Biology Lab Rules
- Cheating is strictly prohibited and results in a failing grade for the lab course.
- Labs are open Monday-Thursday from 9:00 AM to 9:00 PM, and Friday from 9:00 AM to 4:00 PM, with extended hours during the summer.
- Open study is available during these hours, times are posted outside the lab.
- Labs are closed on weekends and holidays.
- Consult with your instructor or an instructional assistant if you have questions.
- Sign the lab policy document and return it to your instructor.
Lab 1: Terminology and Microscopy Review
- This lab focuses on anatomical terminology and microscopes.
- Learn how to use anatomical terms and apply them to body regions.
- Identify body cavities and quadrants.
- Understand the parts and functions of a compound light microscope.
- Properly use and stow the microscope.
Exercise One: Terminology
- Directional terms are used to describe locations on the body.
- Superior means above.
- Inferior means below.
- Dorsal means towards the spinal side.
- Ventral means towards the abdominal side.
- Anterior means in front of.
- ** Posterior** means behind.
- Medial means towards the midline.
- Lateral means away from the midline (left or right).
- Proximal means towards the point of attachment or origin.
- ** Distal** means away from the point of attachment or origin.
- Superficial means at or towards the surface.
- Deep means away from or below the surface.
- Anterior and ventral are interchangeable in humans.
- Posterior and dorsal are interchangeable in humans.
Activity 2: Anatomical Regions
- Regional terms identify specific areas of the body.
- Cephalic: head
- Orbital: eye socket
- Mental: chin
- Cervical: neck
- Thoracic: chest
- Sternal: center of the chest
- Abdominal: belly
- Inguinal: groin
- Pubic: above the genitals
- Vertebral: spinal column
- Lumbar: lower back
- Gluteal: buttock
- Coxal: hip
- Axillary: armpit
- Brachial: upper arm
- Antecubital: front of the elbow
- Antebrachial: forearm
- Carpal: wrist
- Palmar: palm
- Digital: fingers or toes
- Femoral: thigh
- Patellar: knee
- Popliteal: back of the knee
- Tarsal: back part of the foot
- Pedal: foot
- Plantar: sole of the foot
- Calcaneal: heel of the foot
Activity 3: Body Planes
- Body planes are used to view anatomical structures.
- Compound light microscope uses lenses to magnify specimens.
- Working distance: The distance between the objective lens and the specimen.
- Field-of-view: The viewing area seen when looking through the ocular lenses.
- Depth-of-focus: The ability to focus on an entire specimen at one time.
- Parfocal: Ability of the microscope to stay in focus as magnification changes.
- Contrast: The ability to differentiate between light and dark areas.
Activity 4: Proper Use of the Compound Light Microscope
- Start by focusing at low magnification.
- Use coarse focus first, then fine focus.
- Center the specimen using mechanical stage controls.
- If higher magnification is needed, center the specimen and then increase the magnification.
- Adjust lighting as needed.
- Use the lowest magnification necessary for clear viewing.
- Never use coarse focus at high power.
- Clean lenses with lens paper.
Activity 5: Stowing the Compound Light Microscope
- Turn off the light.
- Remove the slide and return it to the slide box.
- Clean and dry the microscope.
- Lower the stage.
- Turn the revolving nosepiece to the low power objective.
- Unplug the cord and wrap it neatly.
- Place the cord on the stage and raise the stage.
- Place the dust cover over the microscope.
- Carry the microscope with two hands and place it in the storage cubicle.
- Place microscopes sideways with six per shelf.
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