Retinal Blood Supply and RPE Structure

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Questions and Answers

Which of the following best describes the structural relationship between Bruch's membrane and the retinal pigment epithelium (RPE)?

  • The RPE is located within Bruch's membrane.
  • Bruch's membrane and the RPE are synonymous structures.
  • Bruch's membrane is located within the RPE.
  • Bruch's membrane separates the RPE from the choriocapillaries. (correct)

What is the primary function of the tight junctions located between the retinal pigment epithelium (RPE) cells?

  • To facilitate the transport of oxygen from the choriocapillaries to the photoreceptors
  • To allow the free passage of blood and fluid between the RPE cells
  • To prevent the entry of blood and fluid from the choriocapillaris, thus maintaining the outer blood-retinal barrier (correct)
  • To promote the phagocytosis of photoreceptor outer segments

Which of the following is the most accurate description of the role of the retinal pigment epithelium (RPE) in the visual cycle?

  • The RPE contains bipolar cells that transmit visual signals to the brain.
  • The RPE converts light into chemical signals.
  • The RPE generates action potentials in response to light.
  • The RPE stores, metabolizes, and transports Vitamin A, essential for phototransduction. (correct)

What is the primary functional consequence of lipofuscin accumulation within the retinal pigment epithelium (RPE)?

<p>Compromised phagocytosis of photoreceptor outer segments (D)</p> Signup and view all the answers

Which of the following best describes the function of zonula occludens in the context of the outer blood-retinal barrier?

<p>They prevent leakage from Bruch's membrane and choriocapillaries between RPE cells. (A)</p> Signup and view all the answers

Which anatomical feature is not associated with the foveola?

<p>Presence of bipolar cells (D)</p> Signup and view all the answers

Why is the integrity of Bruch's membrane important in the context of choroidal neovascularization (CNV)?

<p>It inhibits the formation of new blood vessels from the choroid. (A)</p> Signup and view all the answers

What is the role of lutein and zeaxanthin, found in high concentrations in the macula, in maintaining visual function?

<p>They protect against light-induced oxidative damage. (B)</p> Signup and view all the answers

Which of the following is the most appropriate use for Fundus Autofluorescence (FAF) in clinical practice?

<p>Assessing the function of the retinal pigment epithelium (RPE) (B)</p> Signup and view all the answers

What is the underlying cause of macular edema?

<p>Increased vascular permeability leading to fluid leakage (B)</p> Signup and view all the answers

What is a scotoma, in the context of macular disease symptoms?

<p>Obstruction of central vision (D)</p> Signup and view all the answers

Which of the following best describes the mechanism by which Vascular Endothelial Growth Factor (VEGF) contributes to retinal disease?

<p>VEGF enhances the production of new blood vessels. (D)</p> Signup and view all the answers

Why is fluorescein angiography useful in evaluating retinal and choroidal circulation?

<p>It provides photographic surveillance of fluorescein passage through retinal and choroidal circulations. (B)</p> Signup and view all the answers

What is indicated by the presence of hyperfluorescence due to a 'window defect' in fluorescein angiography?

<p>Atrophy or absence of the RPE (C)</p> Signup and view all the answers

What is the significance of 'pooling' in the context of fluorescein angiography findings?

<p>It represents the accumulation of fluorescein within an anatomical space due to breakdown of OBRB. (B)</p> Signup and view all the answers

What is the most likely cause of hypofluorescence observed during fluorescein angiography?

<p>Blockage of fluorescence by blood or other lesions (D)</p> Signup and view all the answers

What is the primary advantage of using Indocyanine Green Angiography (ICGA) over fluorescein angiography (FA) in evaluating certain retinal conditions?

<p>ICGA penetrates ocular pigments better, allowing for better visualization of the choroidal vasculature. (B)</p> Signup and view all the answers

What is the primary purpose of Optical Coherence Tomography (OCT) angiography?

<p>To visualize the blood flow of the retina and choroid (C)</p> Signup and view all the answers

In the context of fundus autofluorescence (FAF), what does increased autofluorescence typically indicate?

<p>Increased levels of lipofuscin in the RPE (D)</p> Signup and view all the answers

Which of the following is characteristic of cystoid macular edema (CME) as visualized on optical coherence tomography (OCT)?

<p>Presence of intraretinal cysts (A)</p> Signup and view all the answers

Flashcards

Choriocapillaries

Provide blood to the outer layers of the retina, coming from short posterior ciliary arteries.

Central retinal artery

Provides blood to the inner layers of the retina.

Outer retinal barrier

The barrier between Bruch's membrane and RPE with tight junctions, preventing blood/fluid entry.

Inner retinal barrier

Endothelial cells with tight junctions that prevent leakage of blood or fluid.

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RPE and zonula occludens

Outer blood-retinal barrier.

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Zonula occludens

Prevents leakage from Bruch's membrane and choriocapillaries.

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RPE's role in waste management

Phagocytosis and lysosomal degradation of outer segments of photoreceptors; dysfunction leads to lipofuscin accumulation.

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Bruch's membrane

Separates RPE from choriocapillaries; prevents leakage with tight junctions.

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Retinal Landmarks

Macula: 5-6 mm, lutein and zeaxanthin. Fovea: depression center, 1.5 mm. Foveola: thinnest, cones.

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Foveal avascular zone (FAZ)

Zone lacking retinal blood vessels, surrounded by a network of capillaries.

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Macular Edema Cause

Macular Edema from pathologic hyperpermeability of retinal blood vessels.

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Macular disease symptoms

Disruption leads to blurred vision, scotoma, metamorphopsia, micropsia, macropsia, or difficulty with dark adaptation.

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Outer retinal blood barrier

RPE zonula occludens junctions form this barrier.

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Inner retinal blood barrier

Tight junctions between retinal capillary endothelial cells form this barrier

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Fluorescein angiogram (FA)

Surveillance of fluorescein passage through retinal and choroidal circulations.

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Window defect

Hyperfluorescence results from atrophy, macular hole, or RPE tears.

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Pooling

Hyperfluorescence results from breakdown of OBRB (RPE tight junctions).

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Fluorescence

In sub-RPE by pigment epithelial detachment.

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Masking retinal fluorescence

Preretinal lesions (blood) mask retinal fluorescence.

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Hypofluorescence cause

Masking by blood (sub- or intraretinal haemorrhage) or other dense material.

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Study Notes

  • Two arteries supply blood to the retina: choriocapillaries and the central retinal artery.
  • Choriocapillaries supply blood to the outer layers of the retina from short posterior ciliary arteries.
  • The central retinal artery provides blood to the inner layers of the retina.
  • The superficial plexus is in the NFL, and the deep plexus is in the INL.
  • The outer retinal barrier prevents blood or fluid from the choriocapillaries from entering, between Bruch's membrane and RPE with tight junctions.
  • The inner retinal barrier is formed by endothelial cells of the vessel walls, with tight junctions to prevent leakage of blood or fluid.

RPE structure

  • The RPE is a single layer.
  • The cells have an outer non-pigmented basal element with a nucleus.
  • They contain an inner pigmented apical section containing melanosomes, which are more concentrated in the foveal area to absorb more photons.
  • The cell base is in contact with Bruch's membrane.
  • In the posterior pole and fovea, RPE cells are taller, thinner, and more regular with more melanosomes compared to the periphery.

RPE functions

  • The RPE and zonula occludens, which are tight junctions, make up the outer blood-retinal barrier.
  • Zonula occludens prevent leakage from Bruch's membrane and choriocapillaries between RPE cells.
  • Phagocytosis and lysosomal degradation of outer segments of photoreceptors.
  • RPE cells conduct phagocytosis of waste products because of phototransduction.
  • Waste products accumulate, forming lipofuscin when disrupted.
  • The outer blood-retinal barrier is maintained to transport metabolites and metabolic waste products.
  • RPE cells store, metabolize, and transport vitamin A for the visual cycle, aquiring it from choriocapillaries for photoreceptors to enable phototransduction using 11-cis-retinal.
  • The RPE is dense to absorb light.

Bruch's membrane structure

  • Bruch's membrane separates the RPE from the choriocapillaries.
  • It is a barrier that prevents leakage from the choroid to the RPE, and contains tight junctions that make up the outer-retina blood barrier.
  • It is composed of 5 elastic collagen layers.
  • If it breaks, vessels move into the outer and inner retinal layers, and is linked to ARMD and geographic atrophy.
  • Its integrity is important in suppressing choroidal neovascularization (CNV).

Bruch's membrane function

  • The RPE uses Bruch's membrane as a transportation route for waste metabolic products out of the retina.
  • Changes in it are involved in the pathogenesis of macular disorders.

Anatomical landmarks

  • Macula: 5-6 mm diameter, central 10-15 degrees visual field, yellow from xanthophyll, carotenoid pigments lutein and zeaxanthin (higher concentration), than in periphery
  • Fovea: depression center, 1.5 mm diameter
  • Foveola: center of fovea, 0.35 mm diameter, thinnest part of retina, cones, muller cells, no bipolar cells

Foveal avascular zone (FAZ)

  • The foveal avascular zone has no retinal blood vessels but is surrounded by a network of capillaries.
  • Umbo: depression in the center of the foveola, foveal reflex (no FR is an early sign of a problem)

Tests to perform

  • Visual acuities
  • Contrast sensitivity
  • Amsler grid
  • Fundus biomicroscopy
  • Post pole evaluation
  • Peripheral evaluation
  • Fluorescein angiography (FA)
  • Indocyanine green (IG) – choroidal circulation
  • Optical Coherence Tomography (OCT)
  • Fundus Autofluorescence (FAF) –RPE function (loss of autofluorescence)

Macular edema

  • Usually results from pathologic hyperpermeability of retinal blood vessels
  • Increased vascular permeability results in extravasation of fluid, proteins, and macromolecules into the retinal interstitium
  • Contributes to vascular leakage through leucocyte adherence to vessel walls (leukostasis) and is mediated by inflammatory like microphages
  • Molecules that may induce the retinal vascular hyperpermeability include extravasation, prostaglandins (PGD) and leukotrienes, protein kinase C, nitric oxide and various cytokines like vascular endothelial growth factor, and Tumor necrosis factor-alpha, Insulin-like growth factor-1, and Interleukins

Protein kinase C

  • Important involvement in macular ischemic cascade process
  • Also increases in ischemic diseases in retina, associated to oxidative stress like DR, RP, ARMD

Vascular endothelial growth factor (VEGF)

  • There are 2 endothelial growth factors in the RPE: PEGF & VEGF.
  • PEGF maintains equilibrium and prevents the increase in quantity of VEGF because VEGF enhances the production of new vessels

Macular disease symptoms

  • Blurred vision: difficulty w/ close work
  • Scotoma: obstruction of central vision
  • Metamorphopsia: distortion of images
  • Micropsia: decrease in image size, spreading apart foveal cones
  • Macropsia: increase in image size, crowding together foveal cones
  • Difficulty related to dark adaptation: poor vision in dim light

Outer retinal blood barrier

  • Barrier formed by RPE zonula occludens junctions
  • Regulates movement of solutes and nutrients from choroid to subretinal space
  • Fenestrated capillaries allow fluorescein get into the Bruch's membrane, reaching the RPE blocked by tight junctions

Inner retinal blood barrier

  • Barrier form by tight junction between retinal capillary endothelial cells
  • Fluorescein can't pass through the wall of endothelial cells

Fluorescein angiogram (FA)

  • Photographic surveillance of the passage of fluorescein through the retinal and choroidal circulations
  • Administered by IV injection
  • It enters through the ophthalmic artery, passing through the short posterior ciliary artery to reach the choroidal circulation.
  • Choroidal circulation fills ~1 second before retinal circulation (9-15 sec after injection)
  • FAZ: NO vessels
  • Normal FA showing patchy choroidal filling with cilioretinal artery filling (choroidal), arterial phase with choroid and retinal arteries filling, arteriovenous phase with complete arterial/early laminar venous flow, early venous phase with marked laminar venous flow, mid-venous phase showing almost complete venous filling, and late recirculation phase.
  • Blockage of choroidal fluorescence by xanthophyll pigment and melanin in the RPE

Hyperfluorescence

  • A window defect occurs with atrophy/absence of the RPE such as in ARMD, macular hole, RPE tears, unmasking normal choroidal fluorescence, and very early hyperfluorescence due to dry age-related macular degeneration.
  • Pooling occurs in the anatomical space from breakdown of outer blood retinal barrier in the RPE tight junctions or CSR in the subretinal space.

Leakage

  • Early hyperfluorescence
  • Increase in area and intensity
  • From breakdown of the inner BBB
  • Dysfunction or loss of endothelial tight junctions
  • Seen in Proliferative diabetic retinopathy or CRVO, CME or Papilledema.

Hypofluorescence

  • Masking retinal fluorescence by preretinal lesions such as blood (subhyaloid or pre-retinal hemorrhage)
  • Masking of background choroidal fluorescence from Intraretinal hemorrhages, dense exudates: sub-RPE blood or lesions: Increase density of RPE (hypertrophy)
  • Filling defects because of vascular occlusion

Indocyanine Green Angiography

  • Used to evaluate choroidal vascularization and for delineating the choroidal vasculature (masking by the RPE)
  • Penetrates ocular pigments like xanthophyll/Melanin
  • Used for Polypoidal choroidal vasculopathy and Exudative ARMD and Retinal angiomas and Chronic CSC and Posterior uveitis and Choroidal tumors and Breaks in Bruch membrane

Fundus hyperautofluorescence

  • Uses an enhanced fundus camera
  • Permits visualization of accumulated lipofuscin in the RPE
  • Demonstrates expense macular disease
  • Useful in ARMD

Cystoid macular edema

  • Leakage in inner layers of the retina; cysts that appear also

CSCR

  • Showing smokestack or blot of ink and demarcation of the detachment

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