Restriction Fragment Length Polymorphism (RFLP)

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Questions and Answers

What does RFLP stand for?

  • Restriction Fragment Length Polymorphism (correct)
  • Randomized Fragment Length Polymorphism
  • Reactive Fragment Length Polymorphism
  • Regulated Fragment Length Polymorphism

What does RFLP analyze?

  • RNA splicing
  • Genetic variation (correct)
  • Gene expression
  • Protein structure

Which process is used to generate RFLP?

  • Northern blotting
  • PCR amplification
  • Gel electrophoresis
  • Southern blotting (correct)

Flashcards

What is RFLP?

A technique used to analyze genetic variation by cutting DNA with restriction enzymes and comparing the sizes of the resulting fragments.

What does RFLP analyze?

RFLP analyzes genetic variation within a population by examining differences in the length of DNA fragments after cutting with restriction enzymes.

Which process is used to generate RFLP?

Southern blotting is a technique used to transfer DNA fragments from a gel onto a membrane for detection and analysis.

Study Notes

RFLP

  • RFLP stands for Restriction Fragment Length Polymorphism
  • RFLP analyzes genetic variation in DNA sequences by identifying differences in the length of restriction fragments
  • The process used to generate RFLP is DNA digestion with restriction endonucleases, which cut DNA at specific recognition sites, resulting in fragments of varying lengths.

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