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Questions and Answers
What is the main purpose of using restriction endonucleases in clinical and forensic laboratories?
What is the main purpose of using restriction endonucleases in clinical and forensic laboratories?
- To determine the exact location of genes within DNA
- To engineer the construction of recombinant plasmids
- For the molecular characterization of DNA at the sequence-specific level (correct)
- To identify and characterize naturally occurring plasmids
How many base pairs do restriction enzymes commonly used in laboratories have recognition sites for?
How many base pairs do restriction enzymes commonly used in laboratories have recognition sites for?
- Six to eight
- Two to four
- Four to six (correct)
- Seven to ten
What is the process defined as determining where a particular restriction enzyme recognition site is located?
What is the process defined as determining where a particular restriction enzyme recognition site is located?
- Recombinant plasmid identification
- Restriction mapping (correct)
- Plasmid characterization
- DNA engineering
Why were small circular bacterial plasmids initially used in developing restriction maps?
Why were small circular bacterial plasmids initially used in developing restriction maps?
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How is a restriction map typically created?
How is a restriction map typically created?
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