Restriction Endonucleases in Molecular Characterization of DNA
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Questions and Answers

What is the main purpose of using restriction endonucleases in clinical and forensic laboratories?

  • To determine the exact location of genes within DNA
  • To engineer the construction of recombinant plasmids
  • For the molecular characterization of DNA at the sequence-specific level (correct)
  • To identify and characterize naturally occurring plasmids

    • How many base pairs do restriction enzymes commonly used in laboratories have recognition sites for?

  • Six to eight
  • Two to four
  • Four to six (correct)
  • Seven to ten

    • What is the process defined as determining where a particular restriction enzyme recognition site is located?

  • Recombinant plasmid identification
  • Restriction mapping (correct)
  • Plasmid characterization
  • DNA engineering

    • Why were small circular bacterial plasmids initially used in developing restriction maps?

    <p>To identify and characterize naturally occurring plasmids</p> Signup and view all the answers

    How is a restriction map typically created?

    <p>By exposing DNA to multiple restriction enzymes separately and in combinations</p> Signup and view all the answers

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