Regressive Black Oil Soluble Dyes Quiz

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Questions and Answers

Which staining technique involves the use of immunologic and histochemical techniques?

  • Micro-anatomic Staining
  • Histological Staining
  • Histochemical Staining
  • Immunohistochemical Staining (correct)

What is the purpose of staining in histopathology?

  • To identify tissue and cell compartments
  • To check for abnormalities in malignant cells
  • To visualize tissue constituents
  • All of the above (correct)

Which staining technique demonstrates tissue constituents in sections by direct interaction with dye?

  • Histological Staining (correct)
  • Micro-anatomic Staining
  • Histochemical Staining
  • Immunohistochemical Staining

Which staining technique involves the study of tissue constituents through chemical reactions?

<p>Histochemical Staining (A)</p> Signup and view all the answers

Which staining method is used for elastic fibers?

<p>Citric Blue Hemalum Acid (A)</p> Signup and view all the answers

What is the counterstain used after hematoxylin and before methylene blue?

<p>Eosin Y (D)</p> Signup and view all the answers

What is the chief solvent used for stains?

<p>Water (D)</p> Signup and view all the answers

What is the method of staining that requires a mordant and accentuator for staining?

<p>Indirect staining (C)</p> Signup and view all the answers

Which type of dye is commonly used for routine histologic studies?

<p>Hematoxylin (B)</p> Signup and view all the answers

What is the active coloring agent in hematoxylin?

<p>Hematin (D)</p> Signup and view all the answers

Which type of stain is commonly used in histology because it is quick, cheap, and informative?

<p>Hematoxylin (B)</p> Signup and view all the answers

What are mordants in the context of staining?

<p>Substances that combine with the tissue and the staining solution (B)</p> Signup and view all the answers

What is the active mordant in aluminum hematoxylin?

<p>Potassium Aluminum Sulfate (B)</p> Signup and view all the answers

Which of the following is an example of a regressive staining technique?

<p>Supravital staining (D)</p> Signup and view all the answers

Which of the following stains is used to demonstrate calcium deposits?

<p>Acridine Red 3B (B)</p> Signup and view all the answers

Which of the following stains is commonly used for counterstaining?

<p>Eosin Y (C)</p> Signup and view all the answers

Which staining technique is used to demonstrate the general relationship of tissues without necessarily emphasizing inclusion bodies?

<p>Microanatomical staining (A)</p> Signup and view all the answers

Which of the following is an example of a vital staining technique?

<p>Janus green staining (C)</p> Signup and view all the answers

Which stain is the method of choice for demonstrating glycogen?

<p>PAS (D)</p> Signup and view all the answers

Which stain is used primarily for demonstrating acid mucopolysaccharides?

<p>alcian blue (A)</p> Signup and view all the answers

Which stain is suitable for demonstrating proteins in polyacrylamide gels?

<p>Methyl pyronin green (D)</p> Signup and view all the answers

Which stain is the most sensitive for demonstrating lipids?

<p>Sudan black B (B)</p> Signup and view all the answers

Which stain is used for demonstrating RNA in a 2% solution containing 1% lanthanum acetate in 15% acetic acid?

<p>Acridine orange (C)</p> Signup and view all the answers

Which stain is used to detect reticulin fibers and results in black staining?

<p>Gomori’s Silver Impregnation Stain (B)</p> Signup and view all the answers

Which stain is used to detect collagen fibers and results in pink or deep red staining?

<p>Van Gieson’s Stain (B)</p> Signup and view all the answers

Which stain is used to detect collagen fibers and results in blue staining?

<p>Masson’s Trichrome (B)</p> Signup and view all the answers

Which stain is used to detect elastic fibers and results in dark blue or blue-black staining?

<p>Weigert’s Elastic Tissue Stain (B)</p> Signup and view all the answers

Which stain is used to detect myeloid cells and involves peroxidase reaction?

<p>Weigert’s Elastic Tissue Stain (C)</p> Signup and view all the answers

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