Podcast
Questions and Answers
What is the significance of methylated or glucosylated nucleotides in DNA marking?
What is the significance of methylated or glucosylated nucleotides in DNA marking?
- They serve as distinct markers for specific sites on the DNA (correct)
- They interfere with the coding and replicating functions of DNA
- They prevent degradation of DNA in the recipient cell
- They are essential for the action of restriction enzymes
What is the main function of restriction enzymes in DNA cloning technology?
What is the main function of restriction enzymes in DNA cloning technology?
- To catalyze the fusion of foreign DNA to isolated plasmids
- To discriminate between foreign DNA and self DNA based on host-specific markings (correct)
- To degrade foreign DNA in the recipient cell
- To repair DNA molecules that escaped from restriction enzyme action
How does a restriction enzyme recognize DNA that is not properly marked for the recipient cell?
How does a restriction enzyme recognize DNA that is not properly marked for the recipient cell?
- By cutting the DNA at specific sites (correct)
- By catalyzing the fusion of DNA to plasmids
- By degrading the entire DNA molecule
- By repairing the marking on the DNA
What distinguishes restriction endonucleases from other nucleases present in nature?
What distinguishes restriction endonucleases from other nucleases present in nature?
In what scenario might some DNA molecules escape the action of restriction enzymes?
In what scenario might some DNA molecules escape the action of restriction enzymes?
Why did attempts to manipulate the genetic properties of various cells by adding foreign DNA fail initially?
Why did attempts to manipulate the genetic properties of various cells by adding foreign DNA fail initially?
What criteria must foreign DNA meet to be maintained in a cell after being taken up from the outside?
What criteria must foreign DNA meet to be maintained in a cell after being taken up from the outside?
What are replicons in the context of recombinant DNA technology?
What are replicons in the context of recombinant DNA technology?
Which of the following is NOT listed as a type of vector used for carrying foreign DNA fragments?
Which of the following is NOT listed as a type of vector used for carrying foreign DNA fragments?
Why did the genetic modification of organisms become feasible with the development of recombinant DNA technologies?
Why did the genetic modification of organisms become feasible with the development of recombinant DNA technologies?
