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Questions and Answers
Where does the transcription of genes into mRNA occur?
What is the genetic material of most organisms?
What dictates where translation starts and stops?
What is an extrachromosomal genetic element made of a circular DNA molecule?
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In gene expression, which molecules interact with mature mRNA during translation?
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What regulates tissue-specific genes in gene expression?
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What is the purpose of adding restriction enzyme sites to the primers?
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What is the significance of challenging the bacteria with an antibiotic after transformation?
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What does blue-white selection indicate in the cloning process?
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Why is it important to select an expression vector with the same restriction enzyme as used in primer design?
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What is the role of the ampicillin resistance gene in the cloning process?
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What does non-functioning (insertional deactivation) of lacZ gene indicate in recombinant cloning?
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