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Recombinant DNA Technology: Selecting Clones

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Recombinant DNA Technology: Selecting Clones

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Questions and Answers

Where does the transcription of genes into mRNA occur?

Cytoplasm

Mitochondria

Endoplasmic reticulum

Nucleus (correct)

What is the genetic material of most organisms?

rRNA

Plasmid

Chromosome (correct)

mRNA

What dictates where translation starts and stops?

Promoter

rRNA

tRNA

Codons (correct)

What is an extrachromosomal genetic element made of a circular DNA molecule?

Plasmid Signup and view all the answers

In gene expression, which molecules interact with mature mRNA during translation?

tRNA and rRNA complexes Signup and view all the answers

What regulates tissue-specific genes in gene expression?

Tissue-specific promoters Signup and view all the answers

What is the purpose of adding restriction enzyme sites to the primers?

To enable amplification of the gene of interest Signup and view all the answers

What is the significance of challenging the bacteria with an antibiotic after transformation?

To identify which bacteria have taken up and expressed the ampicillin resistance gene Signup and view all the answers

What does blue-white selection indicate in the cloning process?

Improper insertion of the gene of interest Signup and view all the answers

Why is it important to select an expression vector with the same restriction enzyme as used in primer design?

To ensure compatibility for insertion of gene of interest Signup and view all the answers

What is the role of the ampicillin resistance gene in the cloning process?

It enables selection of transformed bacteria Signup and view all the answers

What does non-functioning (insertional deactivation) of lacZ gene indicate in recombinant cloning?

Successful insertion of gene of interest Signup and view all the answers