Podcast
Questions and Answers
Which of the following components is NOT found in Drabkin's reagent used to measure hemoglobin from blood samples?
Which of the following components is NOT found in Drabkin's reagent used to measure hemoglobin from blood samples?
- Potassium cyanide
- Potassium ferricyanide
- Sodium chloride (correct)
- Potassium dihydrogen phosphate (KH2PO4)
What is the role of potassium ferricyanide in measuring hemoglobin using Drabkin's reagent?
What is the role of potassium ferricyanide in measuring hemoglobin using Drabkin's reagent?
- Generates a stable pigment to measure optical density
- Combines with methemoglobin to form cyanmethemoglobin
- Centrifuges the diluted blood to reduce turbidity
- Converts hemoglobin iron from Fe++ to Fe+++ (correct)
What does the color intensity of the mixture measured at a wavelength of 540 nm indicate in hemoglobin measurement?
What does the color intensity of the mixture measured at a wavelength of 540 nm indicate in hemoglobin measurement?
- Formation of cyanmethemoglobin
- Presence of lipemic blood
- Level of WBC count
- Concentration of hemoglobin (correct)
Why can't HbF be used for hemoglobin determination in newborns?
Why can't HbF be used for hemoglobin determination in newborns?
Which form of hemoglobin is NOT measured using the colorimetric method known as Acid Hematin (Sahli’s Method)?
Which form of hemoglobin is NOT measured using the colorimetric method known as Acid Hematin (Sahli’s Method)?
What is the purpose of potassium cyanide in the measurement of hemoglobin using Drabkin's reagent?
What is the purpose of potassium cyanide in the measurement of hemoglobin using Drabkin's reagent?
Which type of blood leads to a false increase in hemoglobin levels when using the colorimetric method?
Which type of blood leads to a false increase in hemoglobin levels when using the colorimetric method?
'Sulfhemoglobin' is excluded when measuring all forms of hemoglobin using which method?
'Sulfhemoglobin' is excluded when measuring all forms of hemoglobin using which method?
'High WBC count' can lead to a false increase in hemoglobin levels due to what factor?
'High WBC count' can lead to a false increase in hemoglobin levels due to what factor?
'Acid Hematin (Sahli’s Method)' uses which reagent for measuring hemoglobin concentration?
'Acid Hematin (Sahli’s Method)' uses which reagent for measuring hemoglobin concentration?
