Rauwolfia: Botanical Overview
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Questions and Answers

What is the primary pharmacological activity of Fagaronine?

  • Antimicrobial activity
  • Antioxidant activity
  • Anti-sickling activity
  • Antimalarial activity (correct)
  • Which of the following acids from Fagara root is associated with anti-sickling activity?

  • Amino acid
  • Ascorbic acid
  • Vanillic acid (correct)
  • Salicylic acid
  • In terms of macroscopic features, what characteristic color does Fagara root exhibit?

  • Yellow (correct)
  • Brown
  • White
  • Red
  • Which species is NOT mentioned as an allied drug to Rauwolfia?

    <p>R.sociabilis (A)</p> Signup and view all the answers

    What is the traditional use of Fagara root?

    <p>As a chewing stick for dental hygiene (A)</p> Signup and view all the answers

    What is the main purpose of leaving some Rauwolfia roots in the ground during collection?

    <p>To avoid the depletion of wild plants (D)</p> Signup and view all the answers

    Which characteristic distinguishes the xylem of Rauwolfia from that of Rauwolfia vomitoria?

    <p>Vessel diameter (B)</p> Signup and view all the answers

    What is the approximate size range of Rauwolfia roots?

    <p>2-10 cm long and 5-22 mm in diameter (B)</p> Signup and view all the answers

    Which of the following statements about the chemical constituents of Rauwolfia is correct?

    <p>Rauwolfia contains 0.7-2.4 % of alkaloids. (D)</p> Signup and view all the answers

    What is a significant reason for encouraging the cultivation of Rauwolfia?

    <p>Wild plant depletion due to high demand (D)</p> Signup and view all the answers

    What distinguishes the structure of the cork in Rauwolfia roots?

    <p>It consists of 2-8 cork zones (C)</p> Signup and view all the answers

    Which alkaloid from Rauwolfia is associated with treating mental disorders?

    <p>Reserpine (B)</p> Signup and view all the answers

    What is the growth pattern of the bark of older Rauwolfia roots?

    <p>It tends to fall off, exposing wood (C)</p> Signup and view all the answers

    Flashcards

    Fagara root

    A small tree, typically 6-12 meters high, with numerous hooked thorns. The dried root of this tree is known as Fagara root and is used in traditional medicine.

    Fagaronine

    An antimalarial alkaloid found in Fagara root, derived from the Zanthoxylum species.

    Berberine

    A type of alkaloid known for its antimicrobial properties, commonly found in Fagara root.

    Chelerythrine

    An alkaloid with antimicrobial activity, found in Fagara root, often used in traditional medicine.

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    Para-hydroxy benzoic acid

    An organic acid with anti-sickling activity, present in Fagara root.

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    What is Rauwolfia?

    The dried rhizome and roots of Rauwolfia serpentina L.Benth.ex Kurz. (Apocynaceae), a small shrub found in India, Pakistan, Burma, Thailand, and Java.

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    How is Rauwolfia collected and prepared?

    It is the process of collecting Rauwolfia from wild plants, leaving some roots in the ground for regeneration, cutting the drug transversely, and drying it. Cultivating Rauwolfia using node or cell cultures is encouraged to protect wild populations and enhance sustainability.

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    Describe the macroscopy of Rauwolfia.

    Rauwolfia root is usually not branched. Older roots have a bark that easily peels off, revealing exposed wood. The younger parts have longitudinal wrinkles, while older parts have ridges. Rhizomes can be differentiated by their small central pith and attached aerial stems.

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    Describe the microscopic features of Rauwolfia root.

    Rauwolfia root has a cork layer divided into 2-8 zones with alternating unlignified and lignified cells. The secondary cortex is filled with parenchyma and starch. The phloem is parenchymatous with sieve tubes and calcium oxalate crystals. Absence of sclerenchyma in the phloem differentiates it from other species like R. vomitoria. The xylem is entirely lignified with 3-6 growth rings, while the secondary xylem contains vessels, fibers, and parenchyma. Vessels in Rauwolfia are less than 57 µm in diameter, a key distinction from R. vomitoria which has vessels exceeding 180 µm. Medullary rays contain starch and alternate with the secondary xylem.

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    What are the chemical constituents of Rauwolfia?

    Rauwolfia contains between 0.7% to 2.4% alkaloids, with over 40 different types. The alkaloidal content is influenced by geographical source, with India and Pakistan being preferred.

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    What are some of the important Rauwolfia alkaloids and their uses?

    Reserpine, one of the main alkaloids, is used to treat hypertension and mental disorders. Rescinnamine is another alkaloid used for hypertension. Ajmaline treats cardiac arrhythmias.

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    What are the main uses of Rauwolfia?

    Rauwolfia is a plant with several medicinal uses. Its roots are used to treat hypertension, mental disorders, and cardiac arrhythmias.

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    Why is the cultivation of Rauwolfia encouraged?

    The depletion of wild Rauwolfia plants due to over-harvesting and its endangered status underscore the importance of sustainable harvesting and cultivating methods, such as nodal or cell cultures, to ensure the continued availability of this valuable medicinal resource.

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    Study Notes

    Rauwolfia: Botanical Definition

    • Rauwolfia consists of dried rhizome and roots of Rauwolfia serpentina L. Benth. ex Kurz (Apocynaceae).
    • It's a small shrub found in India, Pakistan, Burma, Thailand, and Java.

    Rauwolfia: Collection and Preparation

    • Collected mainly from wild plants.
    • Some roots must be left in the ground to allow regeneration.
    • After collection, the drug is cut transversely and dried.
    • Cultivation via nodal and cell culture is encouraged due to:
      • Depletion of wild plants.
      • Destructive nature of root collection.
      • Low seed viability.
      • Endangered species status.

    Rauwolfia: Macroscopy

    • Roots are rarely branched.
    • Bark of older roots readily falls off, exposing wood.
    • Longitudinal wrinkles/ridges found on young and old pieces.
    • Rhizomes differ from roots, having a small central pith and occasional attached aerial stems.
    • Shape: cylindrical or slightly tapering, tortuous pieces.
    • Size: 2-10 cm long, 5-22 mm in diameter.
    • Colour: greyish yellow or brown.
    • Fracture: short.
    • Odour: slight, decreases with age.
    • Taste: bitter.

    Rauwolfia: Microscopy

    • Cork is divided into 2-8 zones with smaller suberized/unlignified cells alternating with larger lignified cells.
    • Secondary cortex is parenchymatous with starch.
    • Phloem is parenchymatous with sieve tubes and calcium oxalate crystals/prisms.
    • Sclerenchyma is absent in the phloem (distinguishes it from other species, e.g., R. vomitoria).
    • Xylem is totally lignified, with 3-6 growth rings.
    • Medullary rays contain starch and alternate with rays of secondary xylem.
    • Secondary xylem consists of vessels, fibres, and parenchyma.
    • Vessels are ≤ 57 µm in diameter (distinguishing it from other species, e.g., R. vomitoria vessels ≤ 180 µm).

    Rauwolfia: Chemical Constituents/Uses

    • Rauwolfia contains 0.7-2.4% alkaloids, consisting of at least 40 different alkaloids.
    • Geographical source affects alkaloid content (India and Pakistan are preferred).
    • Important alkaloids and their uses include:
      • Reserpine: hypertension and mental disorders.
      • Rescinnamine: hypertension.
      • Ajmaline: cardiac arrhythmias.
      • Ajmalicine: blood circulatory diseases.

    Rauwolfia: Allied Drugs

    • 86 Rauwolfia species identified, many used to adulterate R. serpentina.
    • Allied drugs include R. vomitoria and R. tetraphylla.

    Fagara Root: Botanical Definition

    • Fagara root is the dried root of Zanthoxylum zanthoxyloides Waterman and other Zanthoxylum species (Rutaceae).
    • Synonym: Fagara zanthoxyloides Lam.
    • Yoruba name: Orin ata / Igi ata.
    • A small tree (6-12 m high) with many hooked thorns (about 12 mm in length) at the stem base.

    Fagara Root: Macroscopy and Microscopy

    • Shape: cylindrical and tortuous with attached rootlets.
    • Colour: yellow.
    • Taste: peppery and tingling.
    • Cork layer has alternate layers of lignified and unlignified cells.
    • Pericylic fibres are present but less abundant; vessels are numerous.

    Fagara Root: Active Chemical Constituents/Pharmacological Activities

    • Major alkaloids:
      • Fagaronine (antimalarial activity).
      • Berberine (antimicrobial activity).
      • Chelerythrine (antimicrobial activity).
    • Major acids:
      • Para-hydroxy benzoic acid (anti-sickling activity).
      • 2-hydroxymethylbenzoic acid (anti-sickling activity).
      • Vanillic acid (anti-sickling activity).
    • Traditional use: chewing stick for teeth brushing.

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    Description

    This quiz covers the botanical definition, collection, preparation, and macroscopy of Rauwolfia serpentina. Discover the characteristics, morphology, and conservation challenges of this notable medicinal plant, native to parts of Asia. Test your knowledge on this important species and its sustainable practices.

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