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Ralstonia Solanacearum Gene Regulation and Virulence Factors

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Questions and Answers

What is the main function of the acidic EPS1 in the Ralstonia solanacearum-plant interaction?

A major virulence factor, facilitating pathogenesis and contributing to the plant's wilt or death.

What is the primary function of lipopolysaccharide (LPS) in Gram-negative bacteria like R. solanacearum?

As a major component of the outer membrane, it serves as the primary environmental interface.

What is the composition of the O-antigen repeating unit in R. solanacearum's LPS, and how does it contribute to virulence?

The O-antigen repeating unit contains 3 rhamnose and 1 acetylglucosamine molecules, and its unique structure contributes to the bacterium's virulence.

What is the role of flagella in R. solanacearum's pathogenesis, and under what conditions are they produced?

Flagella are not essential for pathogenesis once the bacteria are inside a tomato plant, but are produced in culture at high cell densities (10^7 and 10^9 cells ml^-1) and facilitate motility. Signup and view all the answers

What is the function of the 16-kb eps operon in R. solanacearum, and how does it relate to EPS1 biosynthesis?

The eps operon encodes many proteins involved in the EPS1 biosynthetic pathway, facilitating the production of this critical virulence factor. Signup and view all the answers

What is the significance of the EPS1 polymer in R. solanacearum's pathogenesis, and how much of it is released as cell-free slime?

The EPS1 polymer is a major virulence factor, and approximately 80% of it is released as cell-free slime, facilitating the bacterium's ability to cause disease in plants. Signup and view all the answers

What is the role of pili (fimbriae) in R. solanacearum's pathogenesis, and how do they contribute to virulence?

Pili (fimbriae) are protein appendages that contribute to the bacterium's virulence, although their specific role in pathogenesis is still unclear. Signup and view all the answers

What is the impact of EPS1 mutations on R. solanacearum's ability to cause disease in plants?

Mutants of R. solanacearum unable to produce EPS1 are rarely able to wilt or kill plants, indicating the critical role of EPS1 in pathogenesis. Signup and view all the answers

What is the significance of the R. solanacearum-plant interaction in the context of plant pathology and crop protection?

The R. solanacearum-plant interaction is a critical model system for understanding the mechanisms of pathogenesis and developing strategies for crop protection. Signup and view all the answers

What is the relevance of the Type III Secretion System (T3SS) in R. solanacearum's pathogenesis, and how does it relate to bacterial virulence factors?

The T3SS is a critical component of R. solanacearum's pathogenic machinery, facilitating the delivery of bacterial virulence factors into the plant cell. Signup and view all the answers

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