Ralstonia Solanacearum Gene Regulation and Virulence Factors
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Questions and Answers

What is the main function of the acidic EPS1 in the Ralstonia solanacearum-plant interaction?

A major virulence factor, facilitating pathogenesis and contributing to the plant's wilt or death.

What is the primary function of lipopolysaccharide (LPS) in Gram-negative bacteria like R. solanacearum?

As a major component of the outer membrane, it serves as the primary environmental interface.

What is the composition of the O-antigen repeating unit in R. solanacearum's LPS, and how does it contribute to virulence?

The O-antigen repeating unit contains 3 rhamnose and 1 acetylglucosamine molecules, and its unique structure contributes to the bacterium's virulence.

What is the role of flagella in R. solanacearum's pathogenesis, and under what conditions are they produced?

<p>Flagella are not essential for pathogenesis once the bacteria are inside a tomato plant, but are produced in culture at high cell densities (10^7 and 10^9 cells ml^-1) and facilitate motility.</p> Signup and view all the answers

What is the function of the 16-kb eps operon in R. solanacearum, and how does it relate to EPS1 biosynthesis?

<p>The eps operon encodes many proteins involved in the EPS1 biosynthetic pathway, facilitating the production of this critical virulence factor.</p> Signup and view all the answers

What is the significance of the EPS1 polymer in R. solanacearum's pathogenesis, and how much of it is released as cell-free slime?

<p>The EPS1 polymer is a major virulence factor, and approximately 80% of it is released as cell-free slime, facilitating the bacterium's ability to cause disease in plants.</p> Signup and view all the answers

What is the role of pili (fimbriae) in R. solanacearum's pathogenesis, and how do they contribute to virulence?

<p>Pili (fimbriae) are protein appendages that contribute to the bacterium's virulence, although their specific role in pathogenesis is still unclear.</p> Signup and view all the answers

What is the impact of EPS1 mutations on R. solanacearum's ability to cause disease in plants?

<p>Mutants of R. solanacearum unable to produce EPS1 are rarely able to wilt or kill plants, indicating the critical role of EPS1 in pathogenesis.</p> Signup and view all the answers

What is the significance of the R. solanacearum-plant interaction in the context of plant pathology and crop protection?

<p>The R. solanacearum-plant interaction is a critical model system for understanding the mechanisms of pathogenesis and developing strategies for crop protection.</p> Signup and view all the answers

What is the relevance of the Type III Secretion System (T3SS) in R. solanacearum's pathogenesis, and how does it relate to bacterial virulence factors?

<p>The T3SS is a critical component of R. solanacearum's pathogenic machinery, facilitating the delivery of bacterial virulence factors into the plant cell.</p> Signup and view all the answers

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