Radioisotopes in Immunoassays
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Questions and Answers

What type of light is used in a fluorescent microscope to detect fluorescence?

  • Visible light
  • X-ray light
  • Infrared light
  • UV light (correct)

What is the role of the second antibody in indirect immunofluorescent assays?

  • To enhance the fluorescent signal
  • To inhibit the antigen-antibody reaction
  • To detect antigen-antibody complexes (correct)
  • To label the original antibody

What is the principle of fluorescence polarization immunoassay (FPIA)?

  • The change in polarization of light when an antigen is bound to an antibody (correct)
  • The decrease in fluorescence intensity when an antigen is bound to an antibody
  • The change in wavelength of light when an antigen is bound to an antibody
  • The increase in fluorescence intensity when an antigen is bound to an antibody

What is the purpose of using polystyrene beads in multiplex immunoassay (MIA)?

<p>To act as the solid phase (B)</p> Signup and view all the answers

What is the characteristic of heterogeneous immunoassays?

<p>Requires a solid-phase material (A)</p> Signup and view all the answers

What is the principle of homogeneous EIA?

<p>No physical separation step of bound and labeled antigens is required (C)</p> Signup and view all the answers

What is the purpose of the enzyme label in homogeneous EIA?

<p>To inactivate the reagent antigen when it binds to antibody (C)</p> Signup and view all the answers

What is the characteristic of competitive immunoassays?

<p>Patient antigen competes with labeled antigen for limited antibody-binding sites (B)</p> Signup and view all the answers

What is the advantage of multiplex immunoassay (MIA) technology?

<p>Allows for multiple antibodies or antigens to be detected simultaneously (A)</p> Signup and view all the answers

What is the characteristic of newer, single-use disposable immunoassays?

<p>Is based on immunochromatography (A)</p> Signup and view all the answers

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