10 Questions
What type of light is used in a fluorescent microscope to detect fluorescence?
UV light
What is the role of the second antibody in indirect immunofluorescent assays?
To detect antigen-antibody complexes
What is the principle of fluorescence polarization immunoassay (FPIA)?
The change in polarization of light when an antigen is bound to an antibody
What is the purpose of using polystyrene beads in multiplex immunoassay (MIA)?
To act as the solid phase
What is the characteristic of heterogeneous immunoassays?
Requires a solid-phase material
What is the principle of homogeneous EIA?
No physical separation step of bound and labeled antigens is required
What is the purpose of the enzyme label in homogeneous EIA?
To inactivate the reagent antigen when it binds to antibody
What is the characteristic of competitive immunoassays?
Patient antigen competes with labeled antigen for limited antibody-binding sites
What is the advantage of multiplex immunoassay (MIA) technology?
Allows for multiple antibodies or antigens to be detected simultaneously
What is the characteristic of newer, single-use disposable immunoassays?
Is based on immunochromatography
This quiz covers the uses and limitations of radioisotopes, such as I-125 and I-131, in immunoassays, including chemiluminescent immunoenzymatic assays. Learn about the advantages and disadvantages of using radioisotopes in these assays.
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