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Questions and Answers

What is the diameter of Corynebacteria?

  • 1-2 µm
  • 3-4 µm
  • 0.5-1 µm (correct)
  • 2-3 µm

Which granules are mainly composed of high molecular weight polyphosphate?

  • Metachromatic granules
  • Sulfur granules (correct)
  • Volutin granules
  • Babes-Ernst granules

Which granules are stained deep blue and found in the cytoplasm?

  • Metachromatic granules
  • Volutin granules (correct)
  • Albert granules
  • Sulfur granules

How do individual Corynebacteria in stained smears typically appear?

<p>At acute angles to one another (D)</p> Signup and view all the answers

What is a characteristic appearance of Corynebacteria?

<p>L- or Y-forms (D)</p> Signup and view all the answers

Which method is not used for bacteriological diagnosis?

<p>Genetic sequencing (B)</p> Signup and view all the answers

What type of examination involves the use of a microscope?

<p>Smear examination (D)</p> Signup and view all the answers

Which of these sugars is not fermented by C. diphtheriae?

<p>Lactose (D)</p> Signup and view all the answers

Which type of bacteria is Corynebacterium?

<p>Non-spore-forming, gram-positive bacilli (D)</p> Signup and view all the answers

Where is Corynebacterium commonly found?

<p>Skin and mucous membranes (C)</p> Signup and view all the answers

Which biochemical property is true for C. diphtheriae?

<p>Catalase positive (A)</p> Signup and view all the answers

C. diphtheriae ferments which of the following with acid production?

<p>Maltose (A)</p> Signup and view all the answers

What is a common contaminant of clinical specimens that Corynebacterium is known for?

<p>Contaminants of clinical specimens (B)</p> Signup and view all the answers

What severe disease can the exotoxin produced by Corynebacterium cause?

<p>Diphtheria (C)</p> Signup and view all the answers

For what purpose is Corynebacterium often submitted?

<p>For diagnostic evaluation (B)</p> Signup and view all the answers

What is the initial appearance of C. diphtheriae colonies on Löffler's medium?

<p>Small, opaque, white circular disks with a regular border (C)</p> Signup and view all the answers

Which of these characteristics is true regarding the C. diphtheriae colonies on blood agar?

<p>Small, granular, and gray with irregular edges (C)</p> Signup and view all the answers

What is a characteristic of C. diphtheriae colonies as they evolve on Löffler's medium?

<p>The center becomes elevated and yellow in color (B)</p> Signup and view all the answers

What is one of the criteria used to classify the four biotypes of C. diphtheriae?

<p>Colony morphology (A)</p> Signup and view all the answers

Which of the following is NOT a recognized biotype of C. diphtheriae?

<p>hemolyticus (C)</p> Signup and view all the answers

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Study Notes

Morphology and Identification

  • Corynebacteria are 0.5-1 µm in diameter, non-motile, and do not have capsules.
  • They have an irregular shape, appearing "club-shaped" due to the presence of granules at one end.
  • They are carotenoid-positive and have irregularly distributed granules within the cell.
  • Metachromatic granules or Babes-Ernst granules are composed of high molecular weight polyphosphate and can be present at one or both ends of the cell.
  • Volutin granules stain deep blue and are found in the cytoplasm.
  • Stained smears of corynebacteria often show parallel or acute angles between individual cells.

Diagnostic Laboratory Tests

Characterizations of C. diphtheriae

  • C. diphtheriae grows poorly on Löffler's medium, leading to pleomorphism and distinct morphology.
  • Colonies on Löffler's medium are small, opaque, white, and circular with a regular border that later becomes crenated and elevated with a yellow center.
  • On blood agar, colonies are small, granular, and gray with irregular edges, and may have small zones of hemolysis.
  • There are four recognized biotypes of C. diphtheriae: gravis, mitis, intermedius, and belfanti, classified based on growth characteristics, biochemical reactions, and disease severity.

Diagnostic Laboratory Tests

  • Sterile swabs are taken from the lesion site and can include a portion of the pseudomembrane.
  • Bacteriological diagnosis methods include smear examination, cultural examination, and pathogenicity tests.

Biochemical Reactions

  • C. diphtheriae ferments glucose, galactose, maltose, and dextrin with acid production.
  • It does not ferment lactose, sucrose, or mannitol.
  • It does not liquefy gelatin.
  • It is H2S-positive and catalase-positive.

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