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Questions and Answers

Which property of lipids allows them to function effectively as energy-storage molecules?

  • High solubility in water
  • Stored in the form of triglycerides (correct)
  • High rate of solvation in biological systems
  • Low molecular weight compared to carbohydrates
  • Which of the following best describes the primary function of lipids in biological membranes?

  • Regulating membrane fluidity and permeability (correct)
  • Assisting in hormone production
  • Facilitating transport of carbohydrates
  • Providing rigidity and structural integrity
  • What is a characteristic of fats when compared to carbohydrates after complete oxidation?

  • Contribute more to metabolic processes
  • Produce a higher proportion of water
  • Generate more energy per gram (correct)
  • Generate less energy per molecule
  • What is the role of eicosanoids as lipids?

    <p>Functioning as chemical messengers (D)</p> Signup and view all the answers

    Which mechanism is primarily responsible for the transport of lipids across membranes?

    <p>Simple diffusion through lipid bilayer (B)</p> Signup and view all the answers

    In the context of lipids, what is the significance of iodine values?

    <p>Indicate the degree of unsaturation in fats and oils (B)</p> Signup and view all the answers

    Which of the following statements about the isolation of lipids from egg yolk is incorrect?

    <p>High temperatures improve the extraction rate. (C)</p> Signup and view all the answers

    What effect does osmosis have on red blood cells placed in a hypertonic solution?

    <p>Cells shrink due to loss of water (D)</p> Signup and view all the answers

    What is the primary function of the enzyme PGH synthase in the synthesis of prostaglandins?

    <p>Perform cyclooxygenase and reduce peroxide (C)</p> Signup and view all the answers

    Which type of prostaglandin is specifically known for causing dilation of blood vessels?

    <p>PGE2 (C)</p> Signup and view all the answers

    Which of the following best describes the overall effect of prostaglandins?

    <p>They mediate inflammation and pain. (C)</p> Signup and view all the answers

    What effect does aspirin have on the synthesis of prostaglandins?

    <p>It inhibits the early step in prostaglandin synthesis. (B)</p> Signup and view all the answers

    What role do leukotrienes play in the respiratory system?

    <p>They induce contraction of airway muscles. (C)</p> Signup and view all the answers

    What is the general consequence of overproduction of leukotrienes?

    <p>Respiratory problems, such as asthma attacks. (C)</p> Signup and view all the answers

    What type of structure do thromboxanes have that distinguishes them from other eicosanoids?

    <p>A six-membered ring containing an ether (C)</p> Signup and view all the answers

    What initiates the synthesis of prostaglandins from arachidonic acid?

    <p>PGH synthase (A)</p> Signup and view all the answers

    What primarily influences the fluidity of a membrane?

    <p>The composition of fatty acids and temperature (A)</p> Signup and view all the answers

    How do unsaturated fatty acids contribute to membrane fluidity?

    <p>They introduce kinks that lead to disorder in packing (A)</p> Signup and view all the answers

    What is the characteristic temperature called at which a membrane transitions from a liquid to a crystalline state?

    <p>Transition (melting) temperature (Tm) (B)</p> Signup and view all the answers

    Which of the following factors leads to an increase in the transition temperature (Tm) of a bilayer?

    <p>Increase in fatty acid saturation (A)</p> Signup and view all the answers

    What occurs to lipid mobility when heat is applied to a bilayer?

    <p>Lipid chains increase in disorder (A)</p> Signup and view all the answers

    How do cold-blooded animals maintain membrane fluidity in varying temperatures?

    <p>By modifying the composition of fatty acids in their membranes (D)</p> Signup and view all the answers

    What happens to a membrane containing predominantly saturated fatty acids at lower temperatures?

    <p>It transitions to a more ordered crystalline state (D)</p> Signup and view all the answers

    In response to increased growth temperature, how does E. coli modify its membrane composition?

    <p>By incorporating more saturated fatty acids (A)</p> Signup and view all the answers

    What is the primary function of triacylglycerols in the human body?

    <p>Storage of metabolic energy (B)</p> Signup and view all the answers

    Why do triacylglycerols provide more energy per gram compared to carbohydrates?

    <p>They are more reduced than carbohydrates (A)</p> Signup and view all the answers

    What is the typical energy yield from the complete oxidation of triacylglycerols?

    <p>9 kcal/g (B)</p> Signup and view all the answers

    What is the primary purpose of adding vitamin E to oils used in food?

    <p>To prevent oxidation of double bonds (D)</p> Signup and view all the answers

    How do the energy storage capabilities of triacylglycerols compare to glycogen?

    <p>Triacylglycerols can store energy for significantly longer periods. (A)</p> Signup and view all the answers

    In the alpha nomenclature for fatty acids, which carbon is designated as C-1?

    <p>The carboxyl carbon (A)</p> Signup and view all the answers

    Which type of fatty acids are implicated in an increased risk of cardiovascular diseases due to dietary imbalance?

    <p>Omega-6 fatty acids (D)</p> Signup and view all the answers

    Which of the following correctly describes mixed triglycerides?

    <p>They can contain two or three different fatty acids. (A)</p> Signup and view all the answers

    What important role do triacylglycerols play in the body aside from energy storage?

    <p>They provide insulation and protection to body organs. (C)</p> Signup and view all the answers

    What does omega-3 (ω-3) fatty acid indicate about the first double bond in its structure?

    <p>It is located between the 3rd and 4th carbons from the ω-end (A)</p> Signup and view all the answers

    What commonly found component in the diet of Eskimo tribes contributes to their low heart disease rates?

    <p>Presence of highly unsaturated fatty acids from fish (B)</p> Signup and view all the answers

    What happens to fatty acids that are stored in adipose tissue during starvation?

    <p>They are released and oxidized to provide energy. (D)</p> Signup and view all the answers

    What is the significance of the ratio of energy derivation between TAG and glycogen?

    <p>It suggests the storage efficiency of fats over carbohydrates. (D)</p> Signup and view all the answers

    In omega nomenclature, how is the location of double bonds indicated?

    <p>Using Δ notation relative to the methyl end (B)</p> Signup and view all the answers

    Which of the following fatty acids is an example of omega-3 fatty acid?

    <p>Eicosapentaenoic acid (EPA) (B)</p> Signup and view all the answers

    What potential risk arises from consuming too much fat?

    <p>Increased risk of heart disease (C)</p> Signup and view all the answers

    Which of the following best describes the arrangement of phospholipids in an aqueous environment?

    <p>Phospholipids orient their polar heads towards the aqueous environment and hydrophobic tails away from it. (A)</p> Signup and view all the answers

    What type of structures are primarily formed by glycerophospholipids in an aqueous solution?

    <p>Bilayers (C)</p> Signup and view all the answers

    What is the primary reason phospholipids prefer to form bilayers rather than micelles?

    <p>Steric effects from their two fatty acid chains. (C)</p> Signup and view all the answers

    Which of the following statements about liposomes is accurate?

    <p>Liposomes result from bilayers wrapping around to create a continuous surface. (D)</p> Signup and view all the answers

    What happens when fatty acids reach the critical micelle concentration (CMC)?

    <p>Fatty acids begin to aggregate into micelles. (A)</p> Signup and view all the answers

    Which glycerophospholipid is identified as the major component of most eukaryotic cell membranes?

    <p>Phosphatidylcholine (A)</p> Signup and view all the answers

    How do polar groups of phospholipids behave when in contact with water?

    <p>They interact favorably with water. (A)</p> Signup and view all the answers

    Which of the following lipid structures is typically formed by single-chain lipids?

    <p>Micelles (C)</p> Signup and view all the answers

    Flashcards

    Lipids

    Heterogeneous organic compounds primarily insoluble in water but soluble in organic solvents like ether and chloroform.

    Lipid Function

    Lipids serve as energy storage molecules and chemical messengers in biological systems.

    Lipid Solubility

    Lipids are insoluble in water, but dissolve in organic solvents.

    Energy Storage (Lipid)

    Fats are a major form of stored energy in biological systems, releasing more energy than carbohydrates when fully broken down.

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    Lipid Classes

    Lipids include fats, waxes, oils, hormones, and membrane components.

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    Membrane Structure

    Lipids are a key component of cell membranes.

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    Membrane Function

    The structure of the membrane determines its function.

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    Lipid Isolation

    Lipids can be isolated from food sources like egg yolks.

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    Triacylglycerols

    The most abundant naturally occurring lipids, composed of three fatty acids and glycerol, commonly known as fats (solid) and oils (liquid).

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    Fat Storage

    Triacylglycerols accumulate in adipose tissue, serving as a means of storing fatty acids, especially in animals.

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    Energy Release during Starvation

    During starvation, lipases break down stored fats, releasing fatty acids that are oxidized for energy.

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    Triacylglycerols and Insulation

    Besides energy storage, triacylglycerols provide insulation and protection for body organs.

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    Metabolic Energy Storage

    Triacylglycerols serve as concentrated stores of metabolic energy, with a higher energy yield per gram compared to carbohydrates or proteins.

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    Mixed Triglyceride

    A triglyceride with two or three different fatty acids attached to the glycerol backbone.

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    Simple Triglyceride

    A triglyceride with all three fatty acids being identical.

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    Structure of a Triglyceride

    A triglyceride is comprised of glycerol (pink) with three fatty acids attached.

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    Alpha (α) Nomenclature of Fatty Acids

    A system for naming fatty acids based on the position of double bonds relative to the carboxylic acid end (C-1).

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    Omega (ω) Nomenclature of Fatty Acids

    A system for naming fatty acids based on the position of the first double bond relative to the methyl end (ω-end).

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    ω-3 Fatty Acids

    Fatty acids with the first double bond located three carbons from the methyl end.

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    ω-6 Fatty Acids

    Fatty acids with the first double bond located six carbons from the methyl end.

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    Polyunsaturated Fatty Acids (PUFAs)

    Fatty acids with more than one double bond.

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    Eicosapentenoic Acid (EPA)

    An example of an omega-3 fatty acid.

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    Cardiovascular Diseases

    Diseases related to the heart and blood vessels.

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    Carboxyl Group

    The functional group --COOH, characteristic of carboxylic acids.

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    Prostaglandin Groups

    Prostaglandins are categorized into two main groups: PGE (ether-soluble) and PGF (phosphate buffer-soluble). Each group contains subtypes like PGE1, PGE2, etc.

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    Prostaglandin Function

    Prostaglandins act as chemical messengers in various tissues by regulating the production of cyclic AMP (cAMP), a key intracellular messenger.

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    PGE Effects

    PGE increases fatty acid levels by boosting cAMP synthesis. It plays a role in inflammation.

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    PGE2 Effects

    PGE2 causes blood vessel dilation, activates immune cells, and attracts macrophages to injury sites.

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    Macrophage Role

    Macrophages, attracted by PGE2, contain hydrolytic enzymes that break down tissue at injury sites, leading to water influx and inflammation.

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    PGH Synthase

    Prostaglandins are synthesized from arachidonic acid using an enzyme called PGH synthase. This enzyme has two functions: cyclooxygenase and peroxide reduction.

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    Aspirin's Mode Of Action

    Aspirin inhibits the cyclooxygenase activity of PGH synthase, blocking the early steps of prostaglandin synthesis. This explains its anti-inflammatory effect.

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    Thromboxanes

    Thromboxanes, another type of eicosanoid, contain a unique six-membered ring with an ether. Produced by platelets, they trigger blood clot formation.

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    Glycerophospholipid Structure

    Glycerophospholipids consist of a polar head group attached to a glycerol backbone. They have two fatty acid tails forming a nonpolar region.

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    Phospholipid Bilayer

    Phospholipids spontaneously arrange themselves in a bilayer structure in water. Their polar heads face outward towards water, while their nonpolar tails face inward.

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    Liposome

    A spherical structure formed by a phospholipid bilayer that encloses an internal space. They are commonly used to deliver substances into cells.

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    Micelle

    A spherical structure formed by single-chain lipids, with their polar heads facing outward and their nonpolar tails clustered inward.

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    Critical Micelle Concentration (CMC)

    The concentration of single-chain lipids at which they begin to associate and form micelles.

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    Phosphatidylcholine (Lecithin)

    A major component of most eukaryotic cell membranes, classified as a glycerophospholipid.

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    Phosphatidyl Ethanolamine (Cephalin)

    Another important glycerophospholipid, playing a role in cell structure and signaling.

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    Van der Waals Interactions

    Weak, non-covalent attractions between molecules, essential for stabilizing the structure of phospholipid bilayers.

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    Membrane Fluidity

    The ease with which molecules can move within a membrane. It is influenced by factors such as the presence of unsaturated fatty acids, temperature, and the composition of the membrane.

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    Saturated Fatty Acids

    Fatty acids with only single bonds between carbon atoms. They tend to pack tightly, making the membrane less fluid.

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    Unsaturated Fatty Acids

    Fatty acids that contain double bonds between some of their carbon atoms. The double bonds cause kinks in the fatty acid chain, making them pack less tightly and increasing membrane fluidity.

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    Transition Temperature (Tm)

    The temperature at which a membrane transitions from a more ordered, solid-like state to a more disordered, fluid state.

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    How does temperature affect membrane fluidity?

    At higher temperatures, membranes become more fluid as the lipid molecules move more freely. Conversely, at lower temperatures, membranes become more rigid as the molecules pack more tightly.

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    How do organisms adapt membrane fluidity to temperature changes?

    Organisms can adjust the composition of their membranes by altering the types and amounts of fatty acids present. For example, bacteria and cold-blooded animals increase the proportion of unsaturated fatty acids in their membranes at lower temperatures to maintain fluidity.

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    What happens to the membrane when it transitions from a crystalline state to a liquid crystal state?

    In the crystalline state, fatty acid tails are fully extended and tightly packed, resulting in a rigid membrane. In the liquid crystal state, the tails become more disordered, and the membrane gains fluidity.

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    How does chain length affect membrane fluidity?

    Longer fatty acid chains tend to pack more tightly, making the membrane less fluid. This is because they have more surface area for van der Waals interactions.

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    Study Notes

    Lipids Introduction

    • Lipids are diverse organic compounds insoluble in water but soluble in organic solvents.
    • Examples include fats, waxes, oils, hormones, and membrane components.
    • Lipids function as energy storage molecules and chemical messengers.
    • Fats release more energy than carbohydrates when oxidized completely.
    • Lipids are commonly found in foods like eggs, meat, butter, and dairy products.

    Learning Objectives

    • Students should be able to explain the relationship between lipid structures and functions.
    • Students should be able to explain the relationship between membrane structure and its function.
    • Students should be able to discuss various transport mechanisms across cell membranes.
    • Students should be able to observe and interpret osmotic changes in red blood cells.
    • Students should be able to calculate rates of osmosis.
    • Students should be able to isolate lipids from egg yolks.
    • Students should be able to interpret the results of lipid qualitative tests.
    • Students should be able to determine acid, ester, and iodine values.

    Reference Textbooks

    • "Biochemistry" (7th Ed) by Campbell and Farrell
    • "Biochemistry" (4th Ed) by Voet and Voet
    • "Biochemistry" (7th Ed) by Berg, Tymoczko, and Stryer
    • "Lehninger Principles of Biochemistry" (4th Ed) by Nelson and Cox

    Supplemental Readings and Videos

    • Various online resources about lipids, lipoproteins, eicosanoids, prostaglandins, leukotrienes, and platelet-activating factor are mentioned for further study.

    Classification of Lipids

    • Lipids are classified according to their chemical nature.
      • Certain lipids consist of open-chain compounds with polar head groups and long nonpolar tails.
      • Other lipids consist of fused ring compounds.
    • Simple lipids include esters of fatty acids with various alcohols (e.g., fats and waxes).
    • Complex lipids include esters of fatty acids containing groups in addition to an alcohol and a fatty acid (e.g., phospholipids and glycolipids).
    • Derived lipids are fatty acids, glycerol, steroids/sterols, and fat-soluble vitamins.

    Fatty Acids

    • Fatty acids are the building blocks of many lipids.
    • Fatty acids are carboxylic acids with long aliphatic chains.
    • Chains can be saturated (no double bonds) or unsaturated (one or more double bonds).
    • Most naturally occurring fatty acids have an unbranched chain of an even number of carbon atoms (4-28).
    • Fatty acids are amphipathic: the carboxyl group is hydrophilic, while the hydrocarbon tail is hydrophobic.

    Saturated and Unsaturated Fatty Acids

    • Saturated fatty acids have a linear structure, leading to close packing and high melting points.
    • Unsaturated fatty acids have kinks due to cis double bonds, leading to looser packing and lower melting points.
    • The degree of unsaturation affects the physical properties and interactions of fatty acids.

    Triacylglycerols (Triglycerides)

    • Triglycerides are the most abundant naturally occurring lipids, composed of three fatty acids and glycerol.
    • They are primarily used for energy storage in animals.
    • Triglycerides may be solid (fats) or liquid (oils) at room temperature, depending on the fatty acid composition.
    • Triglycerides can be hydrolyzed (broken down) by using strong bases (saponification) to form soaps and glycerol.

    Glycerophospholipids (Phospholipids)

    • Glycerophospholipids are major components of biological membranes.
    • Phospholipids consist of glycerol, two fatty acids, a phosphate group, and a polar head group.
    • They are amphipathic and form bilayers in water.
    • Examples: phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol.

    Sphingolipids

    • Sphingolipids are derived from sphingosine.
    • They are components of cell membranes.
    • Examples: sphingomyelin, ceramides, and glycosphingolipids.

    Glycolipids

    • Glycolipids are lipids with carbohydrate moieties attached to their core.
    • They are often found in the membrane's outer leaflet.
    • Examples: gangliosides and cerebrosides.

    Waxes

    • Waxes are esters of long-chain fatty acids and long-chain alcohols.
    • They are insoluble in water and have high melting points.
    • Examples of these are plant and animal waxes.

    Steroids

    • Steroids are lipids with a characteristic fused-ring structure.
    • Cholesterol: a crucial component of animal cell membranes.
    • Steroid hormones: derived from cholesterol, possessing diverse functions (e.g., testosterone, estrogen).
    • Bile acids: crucial for fat digestion and absorption.

    Lipid-soluble Vitamins

    • Some vitamins (e.g., vitamins A, D, E, K) are classified as lipids.

    Eicosanoids

    • Eicosanoids are derived from arachidonic acid.
    • Prostaglandins, thromboxanes, and leukotrienes are major classes of eicosanoids.
    • They play roles in inflammation, pain, fever, blood clotting, and other processes.

    Biological Membranes

    • Biological membranes exhibit asymmetry. Proteins, lipids, and carbohydrates are distributed unevenly.
    • Membrane fluidity depends on the composition of fatty acids. Saturated fatty acids and longer hydrocarbon chains decrease fluidity; unsaturated fatty acids, shorter chains, cholesterol increase fluidity.
    • Membrane proteins are either integral or peripheral.

    Membrane Transport

    • Passive transport includes simple and facilitated diffusion.
    • Active transport requires energy and moves molecules against their concentration gradients
      • Primary active transport directly uses ATP.
      • Secondary active transport is powered by ion gradients established by primary active transport.

    Laboratory Activity

    • Students will isolate lipids from egg yolk and perform various experiments to examine membrane transport.

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