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Questions and Answers
Which property of lipids allows them to function effectively as energy-storage molecules?
Which property of lipids allows them to function effectively as energy-storage molecules?
Which of the following best describes the primary function of lipids in biological membranes?
Which of the following best describes the primary function of lipids in biological membranes?
What is a characteristic of fats when compared to carbohydrates after complete oxidation?
What is a characteristic of fats when compared to carbohydrates after complete oxidation?
What is the role of eicosanoids as lipids?
What is the role of eicosanoids as lipids?
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Which mechanism is primarily responsible for the transport of lipids across membranes?
Which mechanism is primarily responsible for the transport of lipids across membranes?
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In the context of lipids, what is the significance of iodine values?
In the context of lipids, what is the significance of iodine values?
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Which of the following statements about the isolation of lipids from egg yolk is incorrect?
Which of the following statements about the isolation of lipids from egg yolk is incorrect?
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What effect does osmosis have on red blood cells placed in a hypertonic solution?
What effect does osmosis have on red blood cells placed in a hypertonic solution?
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What is the primary function of the enzyme PGH synthase in the synthesis of prostaglandins?
What is the primary function of the enzyme PGH synthase in the synthesis of prostaglandins?
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Which type of prostaglandin is specifically known for causing dilation of blood vessels?
Which type of prostaglandin is specifically known for causing dilation of blood vessels?
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Which of the following best describes the overall effect of prostaglandins?
Which of the following best describes the overall effect of prostaglandins?
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What effect does aspirin have on the synthesis of prostaglandins?
What effect does aspirin have on the synthesis of prostaglandins?
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What role do leukotrienes play in the respiratory system?
What role do leukotrienes play in the respiratory system?
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What is the general consequence of overproduction of leukotrienes?
What is the general consequence of overproduction of leukotrienes?
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What type of structure do thromboxanes have that distinguishes them from other eicosanoids?
What type of structure do thromboxanes have that distinguishes them from other eicosanoids?
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What initiates the synthesis of prostaglandins from arachidonic acid?
What initiates the synthesis of prostaglandins from arachidonic acid?
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What primarily influences the fluidity of a membrane?
What primarily influences the fluidity of a membrane?
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How do unsaturated fatty acids contribute to membrane fluidity?
How do unsaturated fatty acids contribute to membrane fluidity?
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What is the characteristic temperature called at which a membrane transitions from a liquid to a crystalline state?
What is the characteristic temperature called at which a membrane transitions from a liquid to a crystalline state?
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Which of the following factors leads to an increase in the transition temperature (Tm) of a bilayer?
Which of the following factors leads to an increase in the transition temperature (Tm) of a bilayer?
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What occurs to lipid mobility when heat is applied to a bilayer?
What occurs to lipid mobility when heat is applied to a bilayer?
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How do cold-blooded animals maintain membrane fluidity in varying temperatures?
How do cold-blooded animals maintain membrane fluidity in varying temperatures?
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What happens to a membrane containing predominantly saturated fatty acids at lower temperatures?
What happens to a membrane containing predominantly saturated fatty acids at lower temperatures?
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In response to increased growth temperature, how does E. coli modify its membrane composition?
In response to increased growth temperature, how does E. coli modify its membrane composition?
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What is the primary function of triacylglycerols in the human body?
What is the primary function of triacylglycerols in the human body?
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Why do triacylglycerols provide more energy per gram compared to carbohydrates?
Why do triacylglycerols provide more energy per gram compared to carbohydrates?
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What is the typical energy yield from the complete oxidation of triacylglycerols?
What is the typical energy yield from the complete oxidation of triacylglycerols?
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What is the primary purpose of adding vitamin E to oils used in food?
What is the primary purpose of adding vitamin E to oils used in food?
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How do the energy storage capabilities of triacylglycerols compare to glycogen?
How do the energy storage capabilities of triacylglycerols compare to glycogen?
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In the alpha nomenclature for fatty acids, which carbon is designated as C-1?
In the alpha nomenclature for fatty acids, which carbon is designated as C-1?
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Which type of fatty acids are implicated in an increased risk of cardiovascular diseases due to dietary imbalance?
Which type of fatty acids are implicated in an increased risk of cardiovascular diseases due to dietary imbalance?
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Which of the following correctly describes mixed triglycerides?
Which of the following correctly describes mixed triglycerides?
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What important role do triacylglycerols play in the body aside from energy storage?
What important role do triacylglycerols play in the body aside from energy storage?
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What does omega-3 (ω-3) fatty acid indicate about the first double bond in its structure?
What does omega-3 (ω-3) fatty acid indicate about the first double bond in its structure?
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What commonly found component in the diet of Eskimo tribes contributes to their low heart disease rates?
What commonly found component in the diet of Eskimo tribes contributes to their low heart disease rates?
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What happens to fatty acids that are stored in adipose tissue during starvation?
What happens to fatty acids that are stored in adipose tissue during starvation?
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What is the significance of the ratio of energy derivation between TAG and glycogen?
What is the significance of the ratio of energy derivation between TAG and glycogen?
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In omega nomenclature, how is the location of double bonds indicated?
In omega nomenclature, how is the location of double bonds indicated?
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Which of the following fatty acids is an example of omega-3 fatty acid?
Which of the following fatty acids is an example of omega-3 fatty acid?
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What potential risk arises from consuming too much fat?
What potential risk arises from consuming too much fat?
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Which of the following best describes the arrangement of phospholipids in an aqueous environment?
Which of the following best describes the arrangement of phospholipids in an aqueous environment?
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What type of structures are primarily formed by glycerophospholipids in an aqueous solution?
What type of structures are primarily formed by glycerophospholipids in an aqueous solution?
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What is the primary reason phospholipids prefer to form bilayers rather than micelles?
What is the primary reason phospholipids prefer to form bilayers rather than micelles?
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Which of the following statements about liposomes is accurate?
Which of the following statements about liposomes is accurate?
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What happens when fatty acids reach the critical micelle concentration (CMC)?
What happens when fatty acids reach the critical micelle concentration (CMC)?
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Which glycerophospholipid is identified as the major component of most eukaryotic cell membranes?
Which glycerophospholipid is identified as the major component of most eukaryotic cell membranes?
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How do polar groups of phospholipids behave when in contact with water?
How do polar groups of phospholipids behave when in contact with water?
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Which of the following lipid structures is typically formed by single-chain lipids?
Which of the following lipid structures is typically formed by single-chain lipids?
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Flashcards
Lipids
Lipids
Heterogeneous organic compounds primarily insoluble in water but soluble in organic solvents like ether and chloroform.
Lipid Function
Lipid Function
Lipids serve as energy storage molecules and chemical messengers in biological systems.
Lipid Solubility
Lipid Solubility
Lipids are insoluble in water, but dissolve in organic solvents.
Energy Storage (Lipid)
Energy Storage (Lipid)
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Lipid Classes
Lipid Classes
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Membrane Structure
Membrane Structure
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Membrane Function
Membrane Function
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Lipid Isolation
Lipid Isolation
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Triacylglycerols
Triacylglycerols
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Fat Storage
Fat Storage
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Energy Release during Starvation
Energy Release during Starvation
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Triacylglycerols and Insulation
Triacylglycerols and Insulation
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Metabolic Energy Storage
Metabolic Energy Storage
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Mixed Triglyceride
Mixed Triglyceride
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Simple Triglyceride
Simple Triglyceride
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Structure of a Triglyceride
Structure of a Triglyceride
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Alpha (α) Nomenclature of Fatty Acids
Alpha (α) Nomenclature of Fatty Acids
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Omega (ω) Nomenclature of Fatty Acids
Omega (ω) Nomenclature of Fatty Acids
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ω-3 Fatty Acids
ω-3 Fatty Acids
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ω-6 Fatty Acids
ω-6 Fatty Acids
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Polyunsaturated Fatty Acids (PUFAs)
Polyunsaturated Fatty Acids (PUFAs)
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Eicosapentenoic Acid (EPA)
Eicosapentenoic Acid (EPA)
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Cardiovascular Diseases
Cardiovascular Diseases
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Carboxyl Group
Carboxyl Group
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Prostaglandin Groups
Prostaglandin Groups
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Prostaglandin Function
Prostaglandin Function
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PGE Effects
PGE Effects
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PGE2 Effects
PGE2 Effects
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Macrophage Role
Macrophage Role
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PGH Synthase
PGH Synthase
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Aspirin's Mode Of Action
Aspirin's Mode Of Action
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Thromboxanes
Thromboxanes
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Glycerophospholipid Structure
Glycerophospholipid Structure
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Phospholipid Bilayer
Phospholipid Bilayer
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Liposome
Liposome
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Micelle
Micelle
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Critical Micelle Concentration (CMC)
Critical Micelle Concentration (CMC)
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Phosphatidylcholine (Lecithin)
Phosphatidylcholine (Lecithin)
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Phosphatidyl Ethanolamine (Cephalin)
Phosphatidyl Ethanolamine (Cephalin)
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Van der Waals Interactions
Van der Waals Interactions
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Membrane Fluidity
Membrane Fluidity
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Saturated Fatty Acids
Saturated Fatty Acids
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Unsaturated Fatty Acids
Unsaturated Fatty Acids
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Transition Temperature (Tm)
Transition Temperature (Tm)
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How does temperature affect membrane fluidity?
How does temperature affect membrane fluidity?
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How do organisms adapt membrane fluidity to temperature changes?
How do organisms adapt membrane fluidity to temperature changes?
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What happens to the membrane when it transitions from a crystalline state to a liquid crystal state?
What happens to the membrane when it transitions from a crystalline state to a liquid crystal state?
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How does chain length affect membrane fluidity?
How does chain length affect membrane fluidity?
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Study Notes
Lipids Introduction
- Lipids are diverse organic compounds insoluble in water but soluble in organic solvents.
- Examples include fats, waxes, oils, hormones, and membrane components.
- Lipids function as energy storage molecules and chemical messengers.
- Fats release more energy than carbohydrates when oxidized completely.
- Lipids are commonly found in foods like eggs, meat, butter, and dairy products.
Learning Objectives
- Students should be able to explain the relationship between lipid structures and functions.
- Students should be able to explain the relationship between membrane structure and its function.
- Students should be able to discuss various transport mechanisms across cell membranes.
- Students should be able to observe and interpret osmotic changes in red blood cells.
- Students should be able to calculate rates of osmosis.
- Students should be able to isolate lipids from egg yolks.
- Students should be able to interpret the results of lipid qualitative tests.
- Students should be able to determine acid, ester, and iodine values.
Reference Textbooks
- "Biochemistry" (7th Ed) by Campbell and Farrell
- "Biochemistry" (4th Ed) by Voet and Voet
- "Biochemistry" (7th Ed) by Berg, Tymoczko, and Stryer
- "Lehninger Principles of Biochemistry" (4th Ed) by Nelson and Cox
Supplemental Readings and Videos
- Various online resources about lipids, lipoproteins, eicosanoids, prostaglandins, leukotrienes, and platelet-activating factor are mentioned for further study.
Classification of Lipids
- Lipids are classified according to their chemical nature.
- Certain lipids consist of open-chain compounds with polar head groups and long nonpolar tails.
- Other lipids consist of fused ring compounds.
- Simple lipids include esters of fatty acids with various alcohols (e.g., fats and waxes).
- Complex lipids include esters of fatty acids containing groups in addition to an alcohol and a fatty acid (e.g., phospholipids and glycolipids).
- Derived lipids are fatty acids, glycerol, steroids/sterols, and fat-soluble vitamins.
Fatty Acids
- Fatty acids are the building blocks of many lipids.
- Fatty acids are carboxylic acids with long aliphatic chains.
- Chains can be saturated (no double bonds) or unsaturated (one or more double bonds).
- Most naturally occurring fatty acids have an unbranched chain of an even number of carbon atoms (4-28).
- Fatty acids are amphipathic: the carboxyl group is hydrophilic, while the hydrocarbon tail is hydrophobic.
Saturated and Unsaturated Fatty Acids
- Saturated fatty acids have a linear structure, leading to close packing and high melting points.
- Unsaturated fatty acids have kinks due to cis double bonds, leading to looser packing and lower melting points.
- The degree of unsaturation affects the physical properties and interactions of fatty acids.
Triacylglycerols (Triglycerides)
- Triglycerides are the most abundant naturally occurring lipids, composed of three fatty acids and glycerol.
- They are primarily used for energy storage in animals.
- Triglycerides may be solid (fats) or liquid (oils) at room temperature, depending on the fatty acid composition.
- Triglycerides can be hydrolyzed (broken down) by using strong bases (saponification) to form soaps and glycerol.
Glycerophospholipids (Phospholipids)
- Glycerophospholipids are major components of biological membranes.
- Phospholipids consist of glycerol, two fatty acids, a phosphate group, and a polar head group.
- They are amphipathic and form bilayers in water.
- Examples: phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol.
Sphingolipids
- Sphingolipids are derived from sphingosine.
- They are components of cell membranes.
- Examples: sphingomyelin, ceramides, and glycosphingolipids.
Glycolipids
- Glycolipids are lipids with carbohydrate moieties attached to their core.
- They are often found in the membrane's outer leaflet.
- Examples: gangliosides and cerebrosides.
Waxes
- Waxes are esters of long-chain fatty acids and long-chain alcohols.
- They are insoluble in water and have high melting points.
- Examples of these are plant and animal waxes.
Steroids
- Steroids are lipids with a characteristic fused-ring structure.
- Cholesterol: a crucial component of animal cell membranes.
- Steroid hormones: derived from cholesterol, possessing diverse functions (e.g., testosterone, estrogen).
- Bile acids: crucial for fat digestion and absorption.
Lipid-soluble Vitamins
- Some vitamins (e.g., vitamins A, D, E, K) are classified as lipids.
Eicosanoids
- Eicosanoids are derived from arachidonic acid.
- Prostaglandins, thromboxanes, and leukotrienes are major classes of eicosanoids.
- They play roles in inflammation, pain, fever, blood clotting, and other processes.
Biological Membranes
- Biological membranes exhibit asymmetry. Proteins, lipids, and carbohydrates are distributed unevenly.
- Membrane fluidity depends on the composition of fatty acids. Saturated fatty acids and longer hydrocarbon chains decrease fluidity; unsaturated fatty acids, shorter chains, cholesterol increase fluidity.
- Membrane proteins are either integral or peripheral.
Membrane Transport
- Passive transport includes simple and facilitated diffusion.
- Active transport requires energy and moves molecules against their concentration gradients
- Primary active transport directly uses ATP.
- Secondary active transport is powered by ion gradients established by primary active transport.
Laboratory Activity
- Students will isolate lipids from egg yolk and perform various experiments to examine membrane transport.
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