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Questions and Answers
What is the purpose of stacking the gel on top of a membrane after the initial electrophoresis?
What is the purpose of stacking the gel on top of a membrane after the initial electrophoresis?
- To amplify the signal from secondary antibodies
- To block the membrane with an inert protein
- To migrate protein bands onto the membrane (correct)
- To expose the membrane to primary antibodies
What is the function of the inert protein used in blocking the membrane?
What is the function of the inert protein used in blocking the membrane?
- Bind all areas of the membrane not already bound by protein bands (correct)
- Amplify the signal from primary antibodies
- Prevent migration of proteins on the gel
- Enhance the enzymatic reaction of the secondary antibodies
What is the purpose of exposing the membrane to primary antibodies specific to the protein of interest in a Western blot?
What is the purpose of exposing the membrane to primary antibodies specific to the protein of interest in a Western blot?
- Amplify the signal from secondary antibodies
- Bind specifically to the primary antibodies (correct)
- Couple to an enzyme system
- Remove unbound antibodies
What kind of reaction does the covalently linked enzyme (e.g., horseradish peroxidase) perform in Western blot upon addition of a specific substrate?
What kind of reaction does the covalently linked enzyme (e.g., horseradish peroxidase) perform in Western blot upon addition of a specific substrate?
What do the dark bands on an SDS-PAGE gel stained with Coomassie represent?
What do the dark bands on an SDS-PAGE gel stained with Coomassie represent?
How does western blotting aid in determining if a specific protein is present in a sample?
How does western blotting aid in determining if a specific protein is present in a sample?
What happens when a stop codon is encountered in the A-site during translation?
What happens when a stop codon is encountered in the A-site during translation?
What happens to the ribosome after termination of translation?
What happens to the ribosome after termination of translation?
How is the growing peptide transferred during elongation?
How is the growing peptide transferred during elongation?
What is the primary function of the extra positively-charged amino acids on the surface of marine animals' myoglobin?
What is the primary function of the extra positively-charged amino acids on the surface of marine animals' myoglobin?
Which hormone acts on the liver to promote glycogen breakdown and gluconeogenesis when blood glucose levels are low?
Which hormone acts on the liver to promote glycogen breakdown and gluconeogenesis when blood glucose levels are low?
Which type of hormone is insulin?
Which type of hormone is insulin?
Which type of hormone travels through the bloodstream?
Which type of hormone travels through the bloodstream?
Which hormone promotes the generation of energy in the form of ATP in muscle when it is required for exercise?
Which hormone promotes the generation of energy in the form of ATP in muscle when it is required for exercise?
What is the functional role of the hydrogen bond between the hydrogen atom attached to nitrogen of a peptide linkage and the carbonyl atom of the fourth amino acid?
What is the functional role of the hydrogen bond between the hydrogen atom attached to nitrogen of a peptide linkage and the carbonyl atom of the fourth amino acid?
What is the geometric constriction due to in an alpha helix?
What is the geometric constriction due to in an alpha helix?
What is the overall dipole moment in an alpha helix?
What is the overall dipole moment in an alpha helix?
What is the directionality in an alpha helix?
What is the directionality in an alpha helix?
Which amino acid has the greatest tendency to form into an alpha helix?
Which amino acid has the greatest tendency to form into an alpha helix?
How can the free energy change of each amino acid be measured?
How can the free energy change of each amino acid be measured?
What is the end product of glycolysis?
What is the end product of glycolysis?
Which enzyme catalyzes the conversion of glucose-1-phosphate to glucose-6-phosphate for entry into glycolysis?
Which enzyme catalyzes the conversion of glucose-1-phosphate to glucose-6-phosphate for entry into glycolysis?
What initiates the breakdown of glycogen by catalyzing the phosphorolysis process?
What initiates the breakdown of glycogen by catalyzing the phosphorolysis process?
Which molecule is used as energy by glycogen synthase during the synthesis of glycogen?
Which molecule is used as energy by glycogen synthase during the synthesis of glycogen?
What signal indicates to store available glucose as glycogen?
What signal indicates to store available glucose as glycogen?
Which molecule is first produced in the tricarboxylic acid cycle and signals enough substrate for the cycle to proceed?
Which molecule is first produced in the tricarboxylic acid cycle and signals enough substrate for the cycle to proceed?
What is responsible for down-regulating glycolysis when ATP levels are adequate?
What is responsible for down-regulating glycolysis when ATP levels are adequate?
What stimulates the dephosphorylation of enzymes by phosphatase in glycogen metabolism?
What stimulates the dephosphorylation of enzymes by phosphatase in glycogen metabolism?
Which enzyme catalyzes the synthesis of glycogen from glucose-1-phosphate?
Which enzyme catalyzes the synthesis of glycogen from glucose-1-phosphate?
What does phosphoglucomutase convert glucose-1-phosphate to, during glycogen breakdown?
What does phosphoglucomutase convert glucose-1-phosphate to, during glycogen breakdown?
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