22 Questions
What is the primary advantage of using cryo-electron tomography (Cryo-ET) in understanding protein structures?
To directly determine macromolecules within their native cellular environment
What can be improved during protein isolation to obtain better results?
All of the above
What is a characteristic of proteins in cells?
They are present in relatively small quantities
What is the primary goal of cryo-electron tomography?
To determine macromolecules within their native cellular environment
Why is it important to consider protein orientation during imaging?
To improve the resolution of the image
What type of detector is used in cryo-electron tomography?
Direct electron detector
What is a benefit of using cryo-electron tomography in understanding macromolecular structures?
Mapping the structure probability within the cell
What is a characteristic of the interactive landscape in cells?
It is dynamic and interconnected
What is the purpose of using a focused beam of gallium ions in Cryo-FIB milling?
To thin the vitrified sample by removing surface atoms
Why is Cryo-FIB thinning preferred over mechanical sectioning at cryo-temperatures?
It avoids intrinsic cutting artifacts
What problem might arise from the predetermined orientation of regions of interest in vitrified whole organisms like C.elegans?
Orientation may interfere with data collection
What role does reorientation of lamella play in addressing data completeness problems?
It provides access to previously obscured regions
What issue might arise from the arrangement of cellular structures on grids?
Interference with analysis due to similar orientation
What is the function of a platinum coating in Cryo-FIB milling?
To provide structural support during ion beam milling
Which molecular structure is visualized at the highest resolution using cryo-ET according to the provided data?
dNTPase
What is the primary reason for using high pressure freezing (HPF) for samples thicker than 10 µm?
To reduce crystalline ice formation
Which publication discusses the identification of a novel RNAP-ribosome supercomplex?
Cell, 183 (3): 730-738.e13 (2020)
What is the resolution of the Influenza A matrix protein M1 as visualized by cryo-ET?
8.0 Å
What is the expected outcome if crystalline ice forms during plunge freezing?
Damage to the native sample
Which macromolecular structure has a resolution of 7.7 Å as determined by cryo-ET?
Clathrin/AP2 coat assembled on mem.
Which factor is essential to avoid sample damage while preparing thick samples for cryo-ET?
Ensuring high cooling rate
What is the resolution disparity between the SARS-CoV-2 spike glycoprotein and the Reovirus SLP?
1.7 Å
Quiz about the structure and function of proteins, including image resolution and orientation, and techniques for improving protein imaging.
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