Protein Structure and Function

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Questions and Answers

What percentage of the lens is composed of protein at the molecular level?

  • 95%
  • 80%
  • 90% (correct)
  • 70%

What is the primary function of alpha-crystallin in the lens?

  • To facilitate the transmission of light
  • To act as a chaperone and protect target proteins from stress (correct)
  • To regulate the refractive index of the lens
  • To provide structural support to the lens

What determines the specific patterns of twisting and folding of proteins in the lens?

  • The sequence of nucleotides in the DNA
  • The 3D structure of the protein
  • The molecular weight of the protein
  • The sequence of amino acids in the protein (correct)

Which technique is most commonly used to determine the 3D structure of crystallin proteins?

<p>X-ray crystallography (A)</p> Signup and view all the answers

What is a common characteristic shared by alpha, beta, and gamma crystallin proteins?

<p>They are all globular proteins with different isoforms (B)</p> Signup and view all the answers

What is the significance of the shared characteristics among alpha, beta, and gamma crystallin proteins?

<p>They are crucial for our understanding of the normal and abnormal lens (C)</p> Signup and view all the answers

What is the primary reason for the transparent nature of the lens?

<p>The structural regularity of crystallins at the atomic and molecular level (A)</p> Signup and view all the answers

What is the characteristic of the aggregate formed by gamma crystallin?

<p>Monomer with two self-associating domains (A)</p> Signup and view all the answers

What is the effect of disturbed packing of crystallins on light transmission?

<p>It reduces light transmission (C)</p> Signup and view all the answers

What is the characteristic of the secondary structure of alpha crystallin?

<p>Mostly alpha helices (A)</p> Signup and view all the answers

What is the reason for the high density of crystallins in lens cells?

<p>The compact globular structure of crystallins (B)</p> Signup and view all the answers

What is the effect of the presence of enough crystallins in lens cells?

<p>They are packed together at high density (A)</p> Signup and view all the answers

What is the role of α-crystallin in maintaining lens transparency?

<p>It prevents protein aggregation by functioning as a molecular chaperone (B)</p> Signup and view all the answers

What is the characteristic of the dynamic quaternary structure of α-crystallin?

<p>It is highly flexible and undergoes continuous exchange of subunits (D)</p> Signup and view all the answers

What is the advantage of using contrast variation in Small Angle Neutron Scattering (SANS)?

<p>It enables the investigation of the location of target protein binding with high precision (D)</p> Signup and view all the answers

What is the characteristic of α-crystallin aggregates?

<p>They are polydisperse and have different sizes (D)</p> Signup and view all the answers

What is the function of α-crystallin in response to denatured proteins?

<p>It binds to the denatured proteins and prevents their aggregation (D)</p> Signup and view all the answers

What is the molecular weight of α-crystallin?

<p>800 kDa (D)</p> Signup and view all the answers

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