Podcast
Questions and Answers
What is the purpose of posttranslational modifications of proteins?
What is the purpose of posttranslational modifications of proteins?
- To increase protein synthesis
- To decrease protein stability
- To convert proteins to a functional form (correct)
- To prevent protein degradation
Where does trimming of proteins occur?
Where does trimming of proteins occur?
- In the mitochondria
- In the cytoplasm
- In the nucleus
- In the endoplasmic reticulum (ER) or Golgi apparatus (correct)
What is the function of ubiquitination on a target protein?
What is the function of ubiquitination on a target protein?
- Marks the protein for degradation (correct)
- Promotes protein folding
- Enhances protein stability
- Facilitates protein secretion
What is the process that adds a phosphate to serine, threonine, or tyrosine residues?
What is the process that adds a phosphate to serine, threonine, or tyrosine residues?
Which process covalently links the sulfur atoms of two different cysteine residues?
Which process covalently links the sulfur atoms of two different cysteine residues?
Which type of covalent attachment attaches a sugar to a protein?
Which type of covalent attachment attaches a sugar to a protein?
When do cotranslational modifications of proteins occur?
When do cotranslational modifications of proteins occur?
Where are DNA polymerases targeted for proper function?
Where are DNA polymerases targeted for proper function?
What process adds a methyl group, usually at lysine or arginine residues?
What process adds a methyl group, usually at lysine or arginine residues?
What determines whether a protein will undergo posttranslational modification?
What determines whether a protein will undergo posttranslational modification?
In protein targeting, where are insulin molecules typically directed for secretion?
In protein targeting, where are insulin molecules typically directed for secretion?
Which proteins are examples of those that undergo trimming?
Which proteins are examples of those that undergo trimming?
Which cellular compartment do receptors target for proper function?
Which cellular compartment do receptors target for proper function?
What kind of proteins require the assistance of chaperones for proper folding?
What kind of proteins require the assistance of chaperones for proper folding?
What can accumulation of misfolded proteins into aggregates lead to?
What can accumulation of misfolded proteins into aggregates lead to?
What is the main importance of posttranslational modifications of proteins?
What is the main importance of posttranslational modifications of proteins?
How does the cell determine which proteins need to undergo posttranslational modifications?
How does the cell determine which proteins need to undergo posttranslational modifications?
Which type of covalent attachment adds a sugar to a protein?
Which type of covalent attachment adds a sugar to a protein?
What is the function of ubiquitination on a target protein?
What is the function of ubiquitination on a target protein?
Where does trimming of proteins occur?
Where does trimming of proteins occur?
What kind of proteins typically require trimming?
What kind of proteins typically require trimming?
What does covalent addition of one or more chemical groups to a protein include?
What does covalent addition of one or more chemical groups to a protein include?
Which process involves the addition of a methyl group, usually at lysine or arginine residues?
Which process involves the addition of a methyl group, usually at lysine or arginine residues?
What type of covalent attachment attaches a sugar to a protein?
What type of covalent attachment attaches a sugar to a protein?
Where are DNA polymerases targeted for proper function?
Where are DNA polymerases targeted for proper function?
What kind of proteins require the assistance of chaperones for proper folding?
What kind of proteins require the assistance of chaperones for proper folding?
Which cellular compartment do receptors target for proper function?
Which cellular compartment do receptors target for proper function?
What is the function of Sodium chloride in the cell lysis solution?
What is the function of Sodium chloride in the cell lysis solution?
What is the role of Proteinase K in the cell lysis solution?
What is the role of Proteinase K in the cell lysis solution?
What is the function of Tris-HCL in the cell lysis solution?
What is the function of Tris-HCL in the cell lysis solution?
What is the function of Ethylene diamine tetra acetic acid (EDTA) in the cell lysis solution?
What is the function of Ethylene diamine tetra acetic acid (EDTA) in the cell lysis solution?
What is the purpose of combining Phenol: Chloroform: Isoamyl alcohol (25:24:1) in the purification step?
What is the purpose of combining Phenol: Chloroform: Isoamyl alcohol (25:24:1) in the purification step?
What is involved in the first common principle shared by all different DNA extraction protocols?
What is involved in the first common principle shared by all different DNA extraction protocols?
What is NOT a component of cell lysis solution for DNA extraction?
What is NOT a component of cell lysis solution for DNA extraction?
What is NOT a function of sodium dodecyl Sulfate (SDS) in cell lysis solution?
What is NOT a function of sodium dodecyl Sulfate (SDS) in cell lysis solution?
What is NOT an essential ingredient for cell lysis?
What is NOT an essential ingredient for cell lysis?
What does EDTA do to prevent DNA destruction during cell lysis?
What does EDTA do to prevent DNA destruction during cell lysis?
What is the purpose of DNA extraction in a biological sample?
What is the purpose of DNA extraction in a biological sample?
Where is genomic DNA located in eukaryotic cells?
Where is genomic DNA located in eukaryotic cells?
What is the role of histone proteins in relation to DNA?
What is the role of histone proteins in relation to DNA?
In which type of investigations is DNA extraction particularly useful?
In which type of investigations is DNA extraction particularly useful?
What are the main components that need to be separated from DNA during the extraction process?
What are the main components that need to be separated from DNA during the extraction process?
What is the structure of DNA?
What is the structure of DNA?
What does DNA contain that provides instructions for an organism's development and reproduction?
What does DNA contain that provides instructions for an organism's development and reproduction?
What is the function of DNA polymerases?
What is the function of DNA polymerases?
Why is it necessary for DNA to be tightly packed within the nucleus of eukaryotic cells?
Why is it necessary for DNA to be tightly packed within the nucleus of eukaryotic cells?
What do scientists typically use extracted DNA for in medicine and science?
What do scientists typically use extracted DNA for in medicine and science?
