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Questions and Answers
What is the primary mechanism involved in protein precipitation as described in the content?
What is the primary mechanism involved in protein precipitation as described in the content?
- Covalent bonding with organic solvents
- Hydrophobic interactions between proteins
- Complexation with metallic ions (correct)
- Denaturation through temperature changes
Which of the following metallic ions is mentioned as effective for protein precipitation?
Which of the following metallic ions is mentioned as effective for protein precipitation?
- K(I)
- Na(I)
- Ca(II)
- Cu(II) (correct)
What effect does the complexation of metal ions have on proteins?
What effect does the complexation of metal ions have on proteins?
- It results in a radical change in their properties (correct)
- It causes irreversible denaturation
- It enhances the solubility of the protein
- It promotes protein aggregation without modification
What characteristic of aluminum (III) makes it an effective protein precipitant?
What characteristic of aluminum (III) makes it an effective protein precipitant?
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How do metallic ions interact with proteins during precipitation?
How do metallic ions interact with proteins during precipitation?
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Study Notes
Protein Precipitation
- Protein precipitation is a process involving the interaction of proteins with metal ions exhibiting a high charge/radius ratio or strong electrostatic fields.
- Transition metal cations like Cu(II), Ag(I), and Zn(II) possess this property.
- Aluminum(III), owing to its charge and small ionic radius, effectively precipitates proteins as well.
- The interaction between metal ions and proteins involves complex formation, where protein's polar groups act as ligands.
- This complexation significantly alters the protein's properties.
- The interaction between metal ions and proteins is non-specific, affecting both host and microbial proteins at sufficient metal ion concentrations.
- These metal ions bind to functional groups at the active sites of enzymes, potentially inhibiting their activity.
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