Protein Dynamics Quiz
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Questions and Answers

Based on the FRAP profiles shown, which SPT profile can be assigned to Protein X?

  • B
  • C
  • Cannot be determined (correct)
  • A

    • Based on the FRAP profiles shown, which SPT profile can be assigned to Protein Y?

  • B (correct)
  • Cannot be determined
  • A
  • C

    • Based on the FRAP profiles shown, which SPT profile can be assigned to Protein Z?

  • C (correct)
  • Cannot be determined
  • B
  • A

    • Which of the following is true about membrane proteins?

    <p>They can function as transporters, anchors, enzymes, and receptors (A)</p> Signup and view all the answers

    What is the function of the ER signal sequence?

    <p>It is recognized by the SRP and directs proteins to the ER (D)</p> Signup and view all the answers

    What happens to soluble proteins in the ER lumen?

    <p>They are translocated across the ER membrane (C)</p> Signup and view all the answers

    What is the role of signal peptidase?

    <p>It cleaves the N-terminus of a protein (D)</p> Signup and view all the answers

    Which of the following mechanisms is NOT involved in intracellular compartmentalization?

    <p>Transport through cytosol (B)</p> Signup and view all the answers

    What determines whether a protein will be made on a free ribosome in the cytosol or on a bound ribosome at the ER?

    <p>The presence of a signal sequence at the amino terminus (B)</p> Signup and view all the answers

    What is the role of signal peptidase in protein targeting?

    <p>To cleave off the signal peptide at the amino terminus (B)</p> Signup and view all the answers

    How do scientists determine whether a protein gets into an organelle in vitro?

    <p>By centrifuging the mixture and observing sedimentation (D)</p> Signup and view all the answers

    Which technique was used to collect data for the mobility of proteins X, Y, and Z?

    <p>Both FRAP and SPT (C)</p> Signup and view all the answers

    In the lipid bilayer of cell membranes, where are the polar head groups and hydrophobic tails located?

    <p>Polar head groups on the outside, hydrophobic tails on the inside (B)</p> Signup and view all the answers

    Which type of lipid makes the lipid more fluid in the bilayer?

    <p>Lipid with unsaturated tails (A)</p> Signup and view all the answers

    What is the difference between olive oil and butter in terms of their lipid composition?

    <p>Olive oil has more unsaturated fats (D)</p> Signup and view all the answers

    What effect does increasing temperature have on the fluidity of fats in the membrane?

    <p>Increases fluidity (A)</p> Signup and view all the answers

    What is the role of cholesterol in the membrane?

    <p>Increases fluidity (B)</p> Signup and view all the answers

    What can make a membrane more fluid?

    <p>Increasing the amount of cholesterol (D)</p> Signup and view all the answers

    What is the orientation of membranes generally maintained?

    <p>Horizontal orientation (C)</p> Signup and view all the answers

    What happens to phospholipids after synthesis in the ER?

    <p>They flip through the membrane (A)</p> Signup and view all the answers

    What is the role of the enzyme ippase in flipping phospholipids?

    <p>It requires ATP (B)</p> Signup and view all the answers

    Which of the following is true about signal sequences?

    <p>Signal sequences are necessary and sufficient to target a certain organelle. (D)</p> Signup and view all the answers

    What determines whether a protein will be made on a free ribosome in the cytosol or on a bound ribosome at the ER?

    <p>The presence of a signal sequence at the amino terminus. (B)</p> Signup and view all the answers

    What is the role of signal peptidase in protein targeting?

    <p>Signal peptidase cleaves off the signal peptide from a protein. (A)</p> Signup and view all the answers

    What happens to soluble proteins in the ER lumen?

    <p>Soluble proteins in the ER lumen stay in the lumen. (C)</p> Signup and view all the answers

    What is the function of the ER signal sequence?

    <p>The ER signal sequence directs proteins to the ER membrane. (B)</p> Signup and view all the answers

    Which of the following is NOT a mechanism involved in intracellular compartmentalization?

    <p>Transport through the ER membrane (D)</p> Signup and view all the answers

    What is the role of chaperones in protein folding?

    <p>Chaperones guide the proper folding of proteins. (B)</p> Signup and view all the answers

    How do scientists determine whether a protein gets into an organelle in vitro?

    <p>By centrifuging the mixture and observing sedimentation (D)</p> Signup and view all the answers

    What determines the number of times a membrane-bound protein crosses the membrane?

    <p>The presence of hydrophobic start transfer and stop transfer sequences (D)</p> Signup and view all the answers

    What is the consequence of changing the hydrophobic amino acids in the signal sequence to charged amino acids?

    <p>The protein will be targeted to the ER membrane instead of the cytosol. (B)</p> Signup and view all the answers

    Which of the following is true about membrane proteins?

    <p>Membrane proteins function as transporters, anchors, enzymes, and receptors. (C)</p> Signup and view all the answers

    What is the role of signal peptidase in protein targeting?

    <p>Signal peptidase cleaves off the signal sequence from proteins in the endoplasmic reticulum. (D)</p> Signup and view all the answers

    What determines whether a protein will be made on a free ribosome in the cytosol or on a bound ribosome at the ER?

    <p>The presence of a signal sequence on the protein. (C)</p> Signup and view all the answers

    What is the function of the ER signal sequence?

    <p>The ER signal sequence is involved in protein translocation across the ER membrane. (B)</p> Signup and view all the answers

    What happens to soluble proteins in the ER lumen?

    <p>Soluble proteins are retained in the ER lumen. (A)</p> Signup and view all the answers

    What is rough ER?

    <p>Rough ER is a type of organelle involved in protein synthesis. (B)</p> Signup and view all the answers

    What determines whether a protein goes into an organelle or stays in the cytoplasm?

    <p>The presence of a signal sequence on the protein. (C)</p> Signup and view all the answers

    What is the role of signal sequences in protein targeting?

    <p>Signal sequences direct proteins to their appropriate organelles. (A)</p> Signup and view all the answers

    What is the function of signal peptidase?

    <p>Signal peptidase cleaves off the signal sequence from proteins in the endoplasmic reticulum. (A)</p> Signup and view all the answers

    Which of the following mechanisms is NOT involved in intracellular compartmentalization?

    <p>Protein folding (B)</p> Signup and view all the answers

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