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Questions and Answers
What is the primary function of endoribonucleases in mRNA degradation?
What is the primary function of endoribonucleases in mRNA degradation?
- To synthesize RNA in the 5' to 3' direction
- To remove nucleotides from the 3' end of the mRNA
- To catalyze the internal cleavage of mRNA (correct)
- To stabilize mRNA by binding to specific sequences
Which RNA-binding protein is involved in the processing of ribosomal RNA (rRNA)?
Which RNA-binding protein is involved in the processing of ribosomal RNA (rRNA)?
- RNase III (correct)
- Hfq
- RNase E
- CsrA
What is the consequence of deadenylation on mRNA degradation?
What is the consequence of deadenylation on mRNA degradation?
- It removes the secondary structure of the mRNA
- It increases the translation efficiency of the mRNA
- It makes the mRNA more susceptible to exonucleases (correct)
- It stabilizes the mRNA by binding to specific sequences
What is a factor that influences mRNA stability?
What is a factor that influences mRNA stability?
Which exoribonuclease is involved in mRNA degradation and can also synthesize RNA in the 3' to 5' direction?
Which exoribonuclease is involved in mRNA degradation and can also synthesize RNA in the 3' to 5' direction?
What is the direction of mRNA degradation catalyzed by RNase R?
What is the direction of mRNA degradation catalyzed by RNase R?
What is the primary function of the 5' trifosphate group in procaryotic mRNAs?
What is the primary function of the 5' trifosphate group in procaryotic mRNAs?
What is the consequence of the elimination of pyrophosphate dependent on RppH in the 5' terminal?
What is the consequence of the elimination of pyrophosphate dependent on RppH in the 5' terminal?
What is the direction of mRNA degradation catalyzed by exoribonucleases like RNase II, RNase R, and PNPase?
What is the direction of mRNA degradation catalyzed by exoribonucleases like RNase II, RNase R, and PNPase?
What is the role of RNase E in mRNA degradation?
What is the role of RNase E in mRNA degradation?
What is the characteristic of the primary transcript produced by procaryotic mRNA synthesis?
What is the characteristic of the primary transcript produced by procaryotic mRNA synthesis?
What is the consequence of the activity of RNase E on the 5' monophosphate group?
What is the consequence of the activity of RNase E on the 5' monophosphate group?
What is the direction of the activity of exoribonucleases like RNase II, RNase R, and PNPase?
What is the direction of the activity of exoribonucleases like RNase II, RNase R, and PNPase?
What is the role of the 5' trifosphate group in procaryotic mRNAs?
What is the role of the 5' trifosphate group in procaryotic mRNAs?
What is the effect of RppH on the stability of procaryotic mRNAs?
What is the effect of RppH on the stability of procaryotic mRNAs?
What is the function of the stem-loop structure at the 3' end of the primary transcript?
What is the function of the stem-loop structure at the 3' end of the primary transcript?
Which process involves the addition of a poly(A) tail and subsequent 3' to 5' degradation of mRNA in E.coli?
Which process involves the addition of a poly(A) tail and subsequent 3' to 5' degradation of mRNA in E.coli?
What is the difference in mRNA degradation between E.coli and B.subtilis?
What is the difference in mRNA degradation between E.coli and B.subtilis?
What is the consequence of failing to degrade aberrant mRNA products?
What is the consequence of failing to degrade aberrant mRNA products?
What is the role of SmpB-tmRNA in trans-translation?
What is the role of SmpB-tmRNA in trans-translation?
What is the purpose of the poly(A) tail in mRNA degradation?
What is the purpose of the poly(A) tail in mRNA degradation?
What is the difference between the degradation of mRNA in procaryotes and eukaryotes?
What is the difference between the degradation of mRNA in procaryotes and eukaryotes?
What is the consequence of ribosome stalling during translation?
What is the consequence of ribosome stalling during translation?
What is the function of the 3' exonucleases in mRNA degradation?
What is the function of the 3' exonucleases in mRNA degradation?
What is the significance of the 5' triphosphate group in procaryotic mRNA?
What is the significance of the 5' triphosphate group in procaryotic mRNA?
What is the role of the oligoribonuclease in mRNA degradation?
What is the role of the oligoribonuclease in mRNA degradation?
Study Notes
Prokaryotic mRNA Degradation
Endoribonucleases
- Catalyze the internal cleavage of mRNA
- Create a new 5' end that can be targeted by exoribonucleases
- Examples:
- RNase E: a major endoribonuclease involved in mRNA degradation
- RNase III: involved in the processing of ribosomal RNA (rRNA)
RNA-binding Proteins
- Bind to specific sequences or structures on the mRNA
- Can stabilize or destabilize the mRNA
- Examples:
- Hfq: a protein that binds to small RNAs (sRNAs) and helps regulate their activity
- CsrA: a protein that binds to mRNA and affects its translation and stability
Deadenylation
- The removal of the poly(A) tail from the 3' end of the mRNA
- Can lead to mRNA degradation by making the mRNA more susceptible to exonucleases
- In prokaryotes, deadenylation is not a major mechanism of mRNA degradation, but it can still occur
mRNA Stability
- Influenced by factors such as:
- Secondary structure of the mRNA
- Binding of RNA-binding proteins
- Presence of ribosome-binding sites
- Translation efficiency
- mRNAs with shorter half-lives are often degraded more quickly
Exoribonucleases
- Catalyze the removal of nucleotides from the 5' or 3' end of the mRNA
- Can degrade mRNA in a 5' to 3' or 3' to 5' direction
- Examples:
- RNase R: a 3' to 5' exoribonuclease involved in mRNA degradation
- PNPase: a 3' to 5' exoribonuclease that can also synthesize RNA in the 3' to 5' direction
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Description
This quiz covers the mechanisms of mRNA degradation in prokaryotes, including the roles of endoribonucleases, RNA-binding proteins, deadenylation, and exoribonucleases in regulating mRNA stability. It also explores the factors that influence mRNA half-life and degradation. Test your knowledge of prokaryotic mRNA degradation pathways!
