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Questions and Answers
What occurs after mRNA leaves the nucleus?
What occurs after mRNA leaves the nucleus?
Intermediary metabolism refers to the processes of building larger molecules from smaller ones only.
Intermediary metabolism refers to the processes of building larger molecules from smaller ones only.
False
What are the cylindrical structures that organize microtubules during cell division called?
What are the cylindrical structures that organize microtubules during cell division called?
Centrioles
Match the following types of metabolism with their definitions:
Match the following types of metabolism with their definitions:
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The process of _______ involves breaking down larger molecules into smaller simpler ones.
The process of _______ involves breaking down larger molecules into smaller simpler ones.
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Where do amino acids go through folding before becoming proteins?
Where do amino acids go through folding before becoming proteins?
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Cilia and flagella are formed by centrioles to aid in cellular respiration.
Cilia and flagella are formed by centrioles to aid in cellular respiration.
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What is the primary function of microtubules during cell division?
What is the primary function of microtubules during cell division?
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ATP is produced during the _______ of nutrients in the cell.
ATP is produced during the _______ of nutrients in the cell.
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Which is NOT a component of intermediary metabolism?
Which is NOT a component of intermediary metabolism?
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What are the primary components of the cytoskeleton?
What are the primary components of the cytoskeleton?
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The cytosol is the living substance inside the cell that includes all organelles.
The cytosol is the living substance inside the cell that includes all organelles.
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What is the function of ribosomes?
What is the function of ribosomes?
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The smallest elements of the cytoskeleton are called __________.
The smallest elements of the cytoskeleton are called __________.
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What is the primary energy currency for cells?
What is the primary energy currency for cells?
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Match the following cellular structures with their functions:
Match the following cellular structures with their functions:
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Lysosomes are responsible for storing energy in the form of phosphates.
Lysosomes are responsible for storing energy in the form of phosphates.
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What is the primary role of the endoplasmic reticulum?
What is the primary role of the endoplasmic reticulum?
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What must happen to the chemical energy stored in ingested food before it can be used by cells?
What must happen to the chemical energy stored in ingested food before it can be used by cells?
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Microtubules are involved in muscle contraction.
Microtubules are involved in muscle contraction.
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The breakdown of _____ provides energy that can be stored in ATP.
The breakdown of _____ provides energy that can be stored in ATP.
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Which statement best describes the function of the plasma membrane?
Which statement best describes the function of the plasma membrane?
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The __________ provides the genetic blueprint during cell replication.
The __________ provides the genetic blueprint during cell replication.
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Cells 'cash in' ATP to pay for _____ related to structure, function, and growth.
Cells 'cash in' ATP to pay for _____ related to structure, function, and growth.
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Which of the following is NOT a function of the cytoskeleton?
Which of the following is NOT a function of the cytoskeleton?
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Which organelle contains powerful hydrolytic enzymes for breaking down organic molecules?
Which organelle contains powerful hydrolytic enzymes for breaking down organic molecules?
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ATP synthesis occurs without the need for any cellular machinery.
ATP synthesis occurs without the need for any cellular machinery.
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How many ATP molecules are produced per cycle during the Citric Acid Cycle?
How many ATP molecules are produced per cycle during the Citric Acid Cycle?
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Match the following cell organelles with their functions:
Match the following cell organelles with their functions:
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How is energy primarily harnessed by cells from the breakdown of organic molecules?
How is energy primarily harnessed by cells from the breakdown of organic molecules?
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What is produced during the conversion of pyruvate to Acetyl CoA?
What is produced during the conversion of pyruvate to Acetyl CoA?
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What is the starting substance in the Citric Acid Cycle?
What is the starting substance in the Citric Acid Cycle?
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Which of the following accurately describes the total carbon dioxide produced during the Citric Acid Cycle?
Which of the following accurately describes the total carbon dioxide produced during the Citric Acid Cycle?
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What holds immense power in ATP and why is it important?
What holds immense power in ATP and why is it important?
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What role do hydrogen atoms play in the electron transport chain?
What role do hydrogen atoms play in the electron transport chain?
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Which statement about the electrons extracted from NADH and FADH2 in the ETC is correct?
Which statement about the electrons extracted from NADH and FADH2 in the ETC is correct?
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What is the main product of glycolysis?
What is the main product of glycolysis?
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How many ATP molecules are produced directly during glycolysis?
How many ATP molecules are produced directly during glycolysis?
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What is the final product of the reaction between oxaloacetate and Acetyl CoA in the Citric Acid Cycle?
What is the final product of the reaction between oxaloacetate and Acetyl CoA in the Citric Acid Cycle?
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What is the primary role of NADH and FADH2 in cellular respiration?
What is the primary role of NADH and FADH2 in cellular respiration?
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What is the primary purpose of the oxidative phosphorylation stage in cellular respiration?
What is the primary purpose of the oxidative phosphorylation stage in cellular respiration?
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In the context of cellular respiration, where does the conversion of citrate back to oxaloacetate take place?
In the context of cellular respiration, where does the conversion of citrate back to oxaloacetate take place?
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Which of the following diseases results from an absence of the enzyme needed to convert glycogen to glucose?
Which of the following diseases results from an absence of the enzyme needed to convert glycogen to glucose?
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How many NAD+ molecules are reduced to NADH during glycolysis?
How many NAD+ molecules are reduced to NADH during glycolysis?
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Which molecule is produced from the breakdown of a glucose molecule in glycolysis?
Which molecule is produced from the breakdown of a glucose molecule in glycolysis?
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In glycolysis, which process uses energy from broken chemical bonds to produce ATP?
In glycolysis, which process uses energy from broken chemical bonds to produce ATP?
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What type of molecule is glucose before it is broken down during glycolysis?
What type of molecule is glucose before it is broken down during glycolysis?
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During glycolysis, what type of reaction converts glucose into pyruvate?
During glycolysis, what type of reaction converts glucose into pyruvate?
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Pyruvate enters which part of the cell for further processing after glycolysis?
Pyruvate enters which part of the cell for further processing after glycolysis?
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What molecule is pyruvate converted into during pyruvate decarboxylation?
What molecule is pyruvate converted into during pyruvate decarboxylation?
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What is the significance of oxidative phosphorylation in cellular respiration?
What is the significance of oxidative phosphorylation in cellular respiration?
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What occurs to NADH during anaerobic respiration?
What occurs to NADH during anaerobic respiration?
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What mechanism do electrons use when passing through the electron transport chain?
What mechanism do electrons use when passing through the electron transport chain?
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What happens to NADH and FADH2 when they are oxidized during cellular respiration?
What happens to NADH and FADH2 when they are oxidized during cellular respiration?
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In aerobic respiration, what is the final electron acceptor in the electron transport chain?
In aerobic respiration, what is the final electron acceptor in the electron transport chain?
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Which statement is true regarding the electron transport chain (ETC)?
Which statement is true regarding the electron transport chain (ETC)?
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What is the net ATP yield from glycolysis when oxygen is present?
What is the net ATP yield from glycolysis when oxygen is present?
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What energy transformation occurs as electrons move through the electron transport system?
What energy transformation occurs as electrons move through the electron transport system?
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Where is the intermembrane space located in relation to the mitochondrion?
Where is the intermembrane space located in relation to the mitochondrion?
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What is the main role of cholesterol in the plasma membrane?
What is the main role of cholesterol in the plasma membrane?
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Membrane proteins can either span the membrane or be peripheral and do not span it.
Membrane proteins can either span the membrane or be peripheral and do not span it.
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How many ATP are typically produced from one NADH molecule during cellular respiration?
How many ATP are typically produced from one NADH molecule during cellular respiration?
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How many ATP are typically generated from one glucose molecule during aerobic cellular respiration?
How many ATP are typically generated from one glucose molecule during aerobic cellular respiration?
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What can occur with the buildup of lactic acid in muscles during heavy exercise?
What can occur with the buildup of lactic acid in muscles during heavy exercise?
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During which phase of cellular respiration does oxidative phosphorylation take place?
During which phase of cellular respiration does oxidative phosphorylation take place?
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What process primarily occurs when oxygen is limited or unavailable?
What process primarily occurs when oxygen is limited or unavailable?
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What is the average yield of ATP produced from one FADH2 molecule?
What is the average yield of ATP produced from one FADH2 molecule?
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How is energy generated during the TCA cycle?
How is energy generated during the TCA cycle?
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Which statement about ATP synthase is correct?
Which statement about ATP synthase is correct?
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What is the primary role of the proton gradient created by electron transport complexes?
What is the primary role of the proton gradient created by electron transport complexes?
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Which pathway provides the most ATP per glucose molecule during aerobic respiration?
Which pathway provides the most ATP per glucose molecule during aerobic respiration?
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What term describes the movement of lipids and proteins within the plasma membrane?
What term describes the movement of lipids and proteins within the plasma membrane?
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The plasma membrane separates the __________ fluid from the intracellular fluid.
The plasma membrane separates the __________ fluid from the intracellular fluid.
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Match the following types of cell junctions with their functions:
Match the following types of cell junctions with their functions:
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Which statement is true about the composition of the plasma membrane?
Which statement is true about the composition of the plasma membrane?
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Peripheral proteins cross the plasma membrane.
Peripheral proteins cross the plasma membrane.
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What is the effect of enzyme activity on phospholipid head and tail configurations?
What is the effect of enzyme activity on phospholipid head and tail configurations?
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The __________ model describes the ever-changing pattern of proteins embedded in the lipid bilayer.
The __________ model describes the ever-changing pattern of proteins embedded in the lipid bilayer.
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What describes the process in which cells release substances using vesicles?
What describes the process in which cells release substances using vesicles?
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What is the main function of the plasma membrane?
What is the main function of the plasma membrane?
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Phospholipids in the plasma membrane have a hydrophilic head and a hydrophobic tail.
Phospholipids in the plasma membrane have a hydrophilic head and a hydrophobic tail.
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What are the primary components of the plasma membrane?
What are the primary components of the plasma membrane?
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_________ molecules allow the passage of specific ions across the membrane.
_________ molecules allow the passage of specific ions across the membrane.
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Match the following components of the plasma membrane with their descriptions:
Match the following components of the plasma membrane with their descriptions:
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How do phospholipids organize in the plasma membrane?
How do phospholipids organize in the plasma membrane?
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The plasma membrane acts only as a mechanical barrier for the cell.
The plasma membrane acts only as a mechanical barrier for the cell.
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What property of the plasma membrane allows it to be fluid and not rigid?
What property of the plasma membrane allows it to be fluid and not rigid?
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The ________ junctions allow cells to touch and form a barrier against substances.
The ________ junctions allow cells to touch and form a barrier against substances.
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Which cell junction acts like 'spot rivets' to anchor two adjacent cells?
Which cell junction acts like 'spot rivets' to anchor two adjacent cells?
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What is the primary function of tight junctions in epithelial tissue?
What is the primary function of tight junctions in epithelial tissue?
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Collagen is the most abundant protein in the body and provides tensile strength.
Collagen is the most abundant protein in the body and provides tensile strength.
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What type of junction is responsible for the synchronized contraction of muscle tissue?
What type of junction is responsible for the synchronized contraction of muscle tissue?
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Tight junctions are primarily found in ________ tissue.
Tight junctions are primarily found in ________ tissue.
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Match the following types of membrane proteins with their functions:
Match the following types of membrane proteins with their functions:
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Which junction provides a communication pathway between adjacent cells?
Which junction provides a communication pathway between adjacent cells?
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The extracellular matrix is located exclusively on the inside of cells.
The extracellular matrix is located exclusively on the inside of cells.
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What fluid provides a pathway for diffusion of nutrients and wastes between blood and tissue cells?
What fluid provides a pathway for diffusion of nutrients and wastes between blood and tissue cells?
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The head of a phospholipid is negatively charged and therefore __________ with water.
The head of a phospholipid is negatively charged and therefore __________ with water.
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Match the following substances to their roles in the plasma membrane:
Match the following substances to their roles in the plasma membrane:
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What are the three different means by which cells are held together?
What are the three different means by which cells are held together?
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Gap junctions allow for the passage of large molecules between connected cells.
Gap junctions allow for the passage of large molecules between connected cells.
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The strongest fiber found in the extracellular matrix is __________.
The strongest fiber found in the extracellular matrix is __________.
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Match the specialized cell junctions with their characteristics:
Match the specialized cell junctions with their characteristics:
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What type of protein fiber is elastin known for?
What type of protein fiber is elastin known for?
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Desmosomes are primarily responsible for making cell membranes impermeable.
Desmosomes are primarily responsible for making cell membranes impermeable.
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What type of molecules do gap junctions primarily allow to pass?
What type of molecules do gap junctions primarily allow to pass?
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What is the primary function of fibronectin in tissues?
What is the primary function of fibronectin in tissues?
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Name the small structures that allow for communication between cells in gap junctions.
Name the small structures that allow for communication between cells in gap junctions.
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Scars are formed by __________ tissue, which produces collagen.
Scars are formed by __________ tissue, which produces collagen.
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Study Notes
Principles of Cell Theory
- Theodor Schwann established that the cell is the smallest structural and functional unit of life.
- Functional activities of cells are determined by their specific structural properties.
- All organisms' cells exhibit fundamental similarities in structure and function.
- Cells are the building blocks of plants, animals, and bacteria.
Cell Structure and Function: Cytoskeleton
- The human body contains trillions of cells classified into approximately 200 types.
- Three main components of the cytoskeleton: Microtubules, Microfilaments, Intermediate Filaments.
Microtubule
- Largest component, composed of long, hollow tubes made of tubulin.
- Maintain cell shape, coordinate movements, and serve as transport highways for cellular components.
- Form cilia and flagella, enabling movement.
Microfilaments
- Smallest components, formed of actin in helical chains, present in all cell types.
- Crucial for muscle contraction and cellular movement (amoeboid movement in white blood cells).
- Provide mechanical support for structures like microvilli, enhancing surface area.
Intermediate Filaments
- Irregular, threadlike proteins offering mechanical strength.
- Composed of keratin, found in hair and nails.
Cell Structure and Function: Organelles
- Nucleus: Double-layered membrane housing DNA, serving as the genetic blueprint during replication.
- Plasma Membrane: Thin layer enclosing the cell, composed mainly of a phospholipid bilayer; selectively permeable.
- Cytosol: Viscous fluid inside cells, facilitating intracellular reactions and containing enzymes for metabolic processes.
Golgi Complex
- A stack of membrane-enclosed sacs, closely tied to the endoplasmic reticulum (ER).
- Modifies, packages, and sorts newly synthesized proteins for transport.
Endoplasmic Reticulum (ER)
- Fluid-filled membranous system functioning as a protein and lipid production factory.
Rough ER
- Studded with ribosomes, synthesizes proteins for secretion and incorporation into membranes.
Smooth ER
- Lacks ribosomes, packages secretory productions for transport to the Golgi complex, abundant in lipids.
Ribosomes
- Sites of protein synthesis that transcribe DNA to RNA and translate mRNA into proteins.
Cellular Metabolism
- Encompasses all chemical reactions involving the breakdown (catabolism) and building (anabolism) of molecules within cells.
- Intermediary Metabolism: Involves the transformation of small organic molecules, occurring in the cytosol with the aid of numerous enzymes, facilitating degradation, synthesis, and transformation of nutrients.
Centrioles
- Cylindrical structures that organize microtubules during cell division and assembly of the mitotic spindle.
- Essential for the formation of cilia and flagella, contributing to cellular mobility.
ATP
- Primary energy currency of the cell, produced during metabolic processes.### Energy Production in Cells
- The body's energy source is derived from the chemical energy in carbon bonds of food.
- Energy must be extracted and converted into a usable form, specifically high-energy phosphate bonds in Adenosine Triphosphate (ATP).
- The third phosphate in ATP holds significant energy potential, facilitating cellular energy transactions.
- Glucose, a six-carbon molecule, undergoes breakdown to harness energy through ATP production.
Lysosomes
- Lysosomes are small, membrane-bound organelles that function in degradation and digestion.
- They contain powerful hydrolytic enzymes for breaking down organic molecules.
- Act as the cell's digestive system, eliminating foreign substances such as viruses and cellular debris.
- ATP serves as the body's common energy currency, enabling cellular processes like maintaining structure, function, and growth.
Peroxisomes
- Peroxisomes are membrane-enclosed organelles that house oxidative enzymes.
- Involved in various metabolic processes, including the breakdown of fatty acids and the detoxification of harmful substances.
- They play a critical role in maintaining cellular health and energy balance.
Cellular Metabolism: Respiration
-
Glycolysis:
- Breaks down 1 glucose (6 carbon) into 2 pyruvate (3 carbon).
- Involves 10 sequential reactions, generating 2 ATP directly.
- Result: 2 NAD+ converted to 2 NADH.
- Affected by metabolic diseases like McArdle disease, which lacks the enzyme to convert glycogen to glucose.
-
Pyruvate Decarboxylation:
- Converts pyruvate into Acetyl CoA (2 carbon) in the mitochondrial matrix, requiring oxygen.
- Produces CO2 and 1 NADH per pyruvate, totaling 2 NADH for 2 pyruvate.
-
Citric Acid Cycle (Krebs Cycle):
- Consists of 8 enzymatic reactions occurring in the mitochondrial matrix.
- 2 carbon atoms are removed from citrate (6C) to regenerate oxaloacetate (4C).
- Generates 6 NADH and 2 FADH2, along with 2 ATP (1 per cycle).
- CO2 produced during this process comes from the cycle, not from breathing.
- Hydrogen atoms removed from the cycle enter the Electron Transport Chain (ETC) as NADH and FADH2.
- Energy release during this cycle is used to convert GDP to GTP.
Oxidative Phosphorylation: Electron Transport Chain (ETC)
- Electrons extracted from NADH and FADH2 pass through a series of electron carriers on the inner mitochondrial membrane.
- Most ATP generated here; this process is also termed oxidative phosphorylation due to the use of O2.
- NADH yields 2.5 ATP on average, while FADH2 yields 1.5 ATP.
- High-energy electrons drop to lower energy levels as they move through the ETC, releasing energy used to transport H+ ions into the intermembrane space.
- H+ concentration gradient drives ATP synthesis through ATP synthase.
- Excess electrons are accepted by oxygen, forming water.
Cellular Respiration Under Different Conditions
-
Aerobic Conditions:
- Total ATP generated from 1 glucose molecule averages 32.
- Sources: 2 from glycolysis, 2 from TCA cycle, 3 from FADH2, and 25 from NADH.
- Variations in the total ATP yield may occur based on cellular conditions.
- Total ATP generated from 1 glucose molecule averages 32.
-
Anaerobic Conditions:
- Limited or no oxygen leads to the conversion of pyruvate into lactate instead of Acetyl CoA.
- Only glycolysis occurs, yielding less energy compared to aerobic processes.
- Glycolysis requires NAD+; during anaerobic respiration, NADH is reused to convert pyruvate into lactic acid.
- Only 2 ATP are generated under anaerobic conditions.
- Excess lactic acid buildup in muscles can impair function and is considered toxic.
Plasma Membrane Overview
- Also known as the cell membrane, it surrounds every cell and regulates movement in and out of the cell.
- Composed of a phospholipid bilayer, consisting of two layers of phospholipids, proteins, lipids, carbohydrates, and cholesterol.
- Separates extracellular fluid (ECF) from intracellular fluid (ICF).
Functionality
- Acts as a selective barrier, determining cell composition by permitting movement of ions, nutrients, waste products, and secretory molecules.
- Plays a crucial role in cell-to-cell communication within the environment.
Structure and Composition
- Phospholipids are the most abundant components, with approximately 1 billion phospholipid molecules in a typical human cell membrane.
- Phospholipids have a hydrophilic polar head (negatively charged, bonds with water) and two hydrophobic nonpolar fatty acid tails (repels water).
- The lipid bilayer is fluid; phospholipids constantly move and rearrange within their layer.
Fluid Mosaic Model
- Describes the fluid nature of the membrane and the diverse arrangement of proteins embedded within the lipid bilayer.
- The membrane's fluidity allows proteins to float like icebergs in a moving sea of lipids.
Membrane Proteins
- Channels: Permit water-soluble molecules, such as small ions, to pass selectively through the membrane.
- Carrier molecules: Facilitate the transfer of larger specific substances like glucose.
- Docking-marker acceptors: Enable secretory vesicles to fuse with the membrane for processes like exocytosis.
- Membrane-bound enzymes: Participate in signal transmission and are anchored to either side of the membrane.
- Receptors: Bind various molecules (e.g., growth hormones) to facilitate communication between the cell and its environment.
- Cell adhesion molecules (CAMs): Enable cell-to-cell adhesion for structural integrity.
- Peripheral proteins: Reside on one side of the membrane without spanning it, playing various roles without crossing the membrane.
Additional Notes
- Membrane structure contains about 50 times more lipid molecules in comparison to protein molecules, but protein constitutes nearly half of the membrane's mass due to larger size.
- Different proteins confer specific functions and capabilities to cells.
- Cholesterol enhances the fluidity and stability of the membrane by interspersing among phospholipid molecules.
Cell to Cell Adhesion
- Plasma membranes facilitate cell-to-cell adhesion.
- Forms of cell adhesion include:
- Cell Adhesion Molecules (CAMs) - anchored to the membrane.
- Extracellular Molecules (ECMs).
- Specialized cell junctions.
Extracellular Matrix (ECM)
- ECM consists of fibrous proteins in a gel-like interstitial fluid rich in complex carbohydrates.
- Interstitial fluid allows diffusion of nutrients and waste between blood and tissue cells.
- ECM occupies the space and fluid between tissue cells and surrounds all cells.
Types of Extracellular Protein Fibers
-
Collagen
- Strong, cable-like fibers; crucial for tensile strength and most abundant protein in the body.
- Comprises about half of total body protein weight.
- Present in skin, muscle, bone, and tissue; associated with scar formation.
-
Elastin
- Rubber-like protein fibers enabling stretching and recoiling.
- Common in the lungs and heart.
-
Fibronectin
- Promotes cell adhesion and maintains cellular positioning.
- Reduced levels are linked to tumor metastasis, aiding cancer cells in escaping and proliferating.
-
Proteins serve as biological glue for cells.
-
Scant presence in epithelial tissue; abundant in connective tissue.
-
Secretion by fibroblasts, specialized cells producing protein fibers.
Specialized Cell Junctions
-
CAMs provide tissue cohesion, functioning like "Velcro" for adjacent cells.
-
Types of specialized cell junctions include:
-
Desmosomes
- Strongest cell-to-cell junctions, prevalent in tissues subject to stretching (skin, liver, uterus, heart).
- Act as "spot rivets" anchoring adjacent cells.
- Consist of cytoplasm thickening plaques connected internally to keratin filaments and intercellular filaments linking adjacent cells.
-
Tight Junctions
- Adjacent cells tightly bind to seal off passages between them, creating an impermeable barrier.
- Common in epithelial tissues that cover the body and line internal cavities.
- Control the entry of substances into cells.
-
Gap Junctions
- Form tunnels allowing communication between cells without touching.
- Enable faster communication and permit small, water-soluble particles to pass between cells while blocking large molecules.
- Abundant in cardiac and smooth muscle to facilitate synchronized contractions through ion movement.
-
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This quiz explores the foundational concepts of cell theory, highlighting the contributions of scientists like Theodor Schwann. Understand the significance of cells as the basic units of life and their role in biological organisms. Test your knowledge on these fundamental principles.