Primary Hemostasis Lab Assessment

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Questions and Answers

What does the bleeding time test primarily assess?

  • The levels of clotting factors II, VII, IX, and X.
  • The intrinsic and extrinsic coagulation pathways.
  • The functionality of the fibrinolytic system.
  • Platelet number, function, and vessel wall integrity. (correct)

In the Modified Duke Method for bleeding time, what is the typical site of puncture?

  • 15-20mm rounded fatty portion of the earlobe (correct)
  • The ball of the foot.
  • Dorsal side of the hand.
  • Volar surface of the forearm.

What is the normal value (NV) for bleeding time when using the Modified Duke Method?

  • NV: >10 minutes
  • NV: 2-5 minutes
  • NV: 10-15 minutes
  • NV: <8 minutes (correct)

During the Ivy Method for bleeding time assessment, what pressure is maintained using a blood pressure cuff?

<p>40 mmHg (D)</p> Signup and view all the answers

If a patient has a normal platelet count but a prolonged bleeding time, which condition is most likely?

<p>Qualitative platelet defect (C)</p> Signup and view all the answers

The capillary resistance test (fragility test) is designed to detect:

<p>Abnormalities in capillary fragility (D)</p> Signup and view all the answers

In the Positive Pressure Test (Rumpel-Leede/Tourniquet Test), at what pressure is the blood pressure cuff typically set?

<p>80 mmHg (for females) (B)</p> Signup and view all the answers

What observation indicates a positive result in the Rumpel-Leede test?

<p>Formation of petechiae. (D)</p> Signup and view all the answers

In the Negative Pressure Test, what pressure is applied using a suction cup?

<p>200-250 torr (B)</p> Signup and view all the answers

What does clot retraction time primarily evaluate?

<p>The ability of platelets to contract the fibrin clot. (B)</p> Signup and view all the answers

What is a normal range for clot retraction, expressed as a percentage of serum volume released?

<p>NV: 44-67% (C)</p> Signup and view all the answers

Abnormal clot retraction can be associated with:

<p>Thrombocytopenia (B)</p> Signup and view all the answers

What is the normal range for platelet count?

<p>150-450 x 10^10/L (D)</p> Signup and view all the answers

Which of the following is a component of the Rees-Ecker diluting fluid used in the Tonkantin method for manual platelet counts?

<p>Sodium citrate (A)</p> Signup and view all the answers

Which method is considered the reference method for manual platelet counts?

<p>Brecher-Cronkite Method (D)</p> Signup and view all the answers

According to platelet estimation on peripheral blood smear, what does a reported range of 100,000-149,000/uL indicate?

<p>Slightly decreased (C)</p> Signup and view all the answers

At what platelet level is spontaneous bleeding possible?

<p>Less than 30,000/uL (B)</p> Signup and view all the answers

Why does a sample become clearer during platelet aggregation?

<p>Platelets aggregate, allowing more light to pass. (A)</p> Signup and view all the answers

Which aggregating agent typically shows a normal response in patients with Glanzmann thrombasthenia?

<p>Ristocetin (A)</p> Signup and view all the answers

In the Glass Bead Retention Test, what does the procedure assess?

<p>Platelet adhesiveness. (B)</p> Signup and view all the answers

Flashcards

Bleeding Time

The actual time it takes for a standard wound to stop bleeding

Modified Duke Method

A method to measure bleeding time where a 15-20mm cut is made on the earlobe using a sterile blade, and blood is absorbed until bleeding stops. Normal value is less than 8 minutes.

Ivy Method

A method to measure bleeding time involving puncturing the volar surface of the forearm twice under 40 mmHg pressure, collecting blood with filter paper every 30 seconds after 2 minutes.

Capillary Resistance Test

A test that detects abnormalities of capillaries due to structural weakness or thrombocytopenia.

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Positive Pressure Test

A method to measure capillary fragility by applying a blood pressure cuff to the upper arm for 5 minutes at 100 mmHg and observing for petechiae formation after removal.

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Negative Pressure Test

A method to measure capillary fragility by applying a suction cup on the upper arm for 1 minute and counting petechiae after 5 minutes.

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Clot Retraction Time

A test where 5 mL of whole blood is incubated at 37°C for 1 hour, measuring how much the clot retracts from the tube walls.

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Tonkantin Method

A direct method for platelet count using Rees-Ecker as a diluent, a light microscope, and Rees-Ecker Diluting Fluid with sodium citrate, formalin, and Brilliant Cresyl Blue.

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Brecher-Cronkite Method

A direct method for manual platelet count, using 1% ammonium oxalate as diluting fluid.

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Unopette Method

A direct method for platelet count using tripotassium EDTA and ammonium oxalate, hemolyzing red cells to block platelet activity.

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Normal Platelet Count

Normal range: 200,000 - 400,000/uL

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Platelet Aggregation

A test where aggregating agents are added to platelet-rich plasma (PRP) to induce shape change and aggregation, measured by an aggregometer.

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Glass Bead Retention Test

Platelet adhesion.

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Study Notes

  • Lab assessment of primary hemostasis is discussed.

Bleeding Time

  • Actual time it takes for a standard wound to stop bleeding.
  • Detects abnormalities of platelet number and function, Factor VIII: VWF deficiency, and vessel wall structure.

Methods

  • Modified Duke Method involves using a 15-20mm rounded fatty portion of the earlobe.
  • A No. 11 sterile Bard-Parker Surgical Blade is used, with the blade passing through the ear and hitting a glass slide with a clicking sound.
  • Normal value (NV) is less than 8 minutes.

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