Biochem Lecture 10
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Questions and Answers

What is the function of the 5' cap in eukaryotic mRNAs?

  • Protects RNA from degradation by ribosomes
  • Signals the start of translation by forming a binding site for the ribosome (correct)
  • Initiates translation by binding to the nucleus
  • Stabilizes the mRNA by forming a binding site for mitochondria
  • What is the linkage between 7-Methylguanosine and the 5' end of almost all eukaryotic mRNAs?

  • 5'-5' triphosphate linkage (correct)
  • 3'-5' phosphodiester linkage
  • 3'-3' triphosphate linkage
  • 5'-3' phosphodiester linkage
  • What does the presence of introns in genes signify?

  • They are only found in bacteria
  • They prevent gene expression
  • They are present in most genes in vertebrates and some in yeast (correct)
  • They stabilize mRNA molecules
  • Where is the capbinding complex (CBC) associated during the synthesis of the 5' cap?

    <p>Tethered to Pol II through an association with the CTD</p> Signup and view all the answers

    What is one of the post-transcriptional processes involved in RNA processing?

    <p>Splicing out introns</p> Signup and view all the answers

    When does the addition of a 5'-cap occur in eukaryotic cells occur?

    <p>Before synthesis of the primary transcript is complete</p> Signup and view all the answers

    What is the function of splicing in post-transcriptional RNA processing?

    <p>Removing introns and attaching exons</p> Signup and view all the answers

    Where does processing of eukaryotic mRNA take place?

    <p>In the nucleus</p> Signup and view all the answers

    What are the three main processes involved in forming mature mRNA from a primary transcript in eukaryotes?

    <p>Capping, polyadenylation, splicing</p> Signup and view all the answers

    What is the function of a 3’-poly(A) tail in mRNA processing?

    <p>Prevents mRNA degradation</p> Signup and view all the answers

    Where does splicing occur during post-transcriptional RNA processing in eukaryotes?

    <p>Nucleus</p> Signup and view all the answers

    Which process of RNA processing involves removing introns and joining exons together?

    <p>Splicing</p> Signup and view all the answers

    When does the addition of a 5' cap to eukaryotic mRNAs typically occur?

    <p>During transcription</p> Signup and view all the answers

    What is the source of methyl groups often found at the 2' position of the first and second nucleotides in eukaryotic mRNAs?

    <p>S-adenosyl-methionine (SAM)</p> Signup and view all the answers

    Which complex is associated with the capbinding complex (CBC) during the synthesis of the 5' cap?

    <p>Capbinding complex</p> Signup and view all the answers

    What is the role of introns in most genes in vertebrates?

    <p>Contribute to genetic variation</p> Signup and view all the answers

    What post-transcriptional modification is involved in removing introns from pre-mRNA in eukaryotes?

    <p>Splicing</p> Signup and view all the answers

    What is the function of methylguanosine at the 5' end of eukaryotic mRNAs?

    <p>Facilitates translation initiation</p> Signup and view all the answers

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