Biochem Lecture 10
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Questions and Answers

What is the function of the 5' cap in eukaryotic mRNAs?

  • Protects RNA from degradation by ribosomes
  • Signals the start of translation by forming a binding site for the ribosome (correct)
  • Initiates translation by binding to the nucleus
  • Stabilizes the mRNA by forming a binding site for mitochondria

What is the linkage between 7-Methylguanosine and the 5' end of almost all eukaryotic mRNAs?

  • 5'-5' triphosphate linkage (correct)
  • 3'-5' phosphodiester linkage
  • 3'-3' triphosphate linkage
  • 5'-3' phosphodiester linkage

What does the presence of introns in genes signify?

  • They are only found in bacteria
  • They prevent gene expression
  • They are present in most genes in vertebrates and some in yeast (correct)
  • They stabilize mRNA molecules

Where is the capbinding complex (CBC) associated during the synthesis of the 5' cap?

<p>Tethered to Pol II through an association with the CTD (D)</p> Signup and view all the answers

What is one of the post-transcriptional processes involved in RNA processing?

<p>Splicing out introns (B)</p> Signup and view all the answers

When does the addition of a 5'-cap occur in eukaryotic cells occur?

<p>Before synthesis of the primary transcript is complete (A)</p> Signup and view all the answers

What is the function of splicing in post-transcriptional RNA processing?

<p>Removing introns and attaching exons (B)</p> Signup and view all the answers

Where does processing of eukaryotic mRNA take place?

<p>In the nucleus (C)</p> Signup and view all the answers

What are the three main processes involved in forming mature mRNA from a primary transcript in eukaryotes?

<p>Capping, polyadenylation, splicing (D)</p> Signup and view all the answers

What is the function of a 3’-poly(A) tail in mRNA processing?

<p>Prevents mRNA degradation (B)</p> Signup and view all the answers

Where does splicing occur during post-transcriptional RNA processing in eukaryotes?

<p>Nucleus (B)</p> Signup and view all the answers

Which process of RNA processing involves removing introns and joining exons together?

<p>Splicing (C)</p> Signup and view all the answers

When does the addition of a 5' cap to eukaryotic mRNAs typically occur?

<p>During transcription (D)</p> Signup and view all the answers

What is the source of methyl groups often found at the 2' position of the first and second nucleotides in eukaryotic mRNAs?

<p>S-adenosyl-methionine (SAM) (A)</p> Signup and view all the answers

Which complex is associated with the capbinding complex (CBC) during the synthesis of the 5' cap?

<p>Capbinding complex (D)</p> Signup and view all the answers

What is the role of introns in most genes in vertebrates?

<p>Contribute to genetic variation (D)</p> Signup and view all the answers

What post-transcriptional modification is involved in removing introns from pre-mRNA in eukaryotes?

<p>Splicing (C)</p> Signup and view all the answers

What is the function of methylguanosine at the 5' end of eukaryotic mRNAs?

<p>Facilitates translation initiation (C)</p> Signup and view all the answers

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