Lodish Ch 10: Post-transcriptional Gene Control

Pre-mRNPs that are capped, spliced, and cleaved must be _____ before they are called nuclear mRNPs.

The RNA recognition motif is the most common RNA-binding domain in hnRNP proteins. Another name for this motif is:

In an experiment you have used recombinant DNA technology to create hnRNP C protein tagged with green fluorescent protein and hnRNP A1 protein tagged with red fluorescent protein. What would you expect to see when the proteins are expressed and visualized in Xenopus cells?

Mutations that affect the binding of an SR protein to an exonic splicing enhancer can cause exon skipping in some genes, producing mRNAs that when translated yield nonfunctional proteins. In addition to an RNA-binding domain, SR proteins also contain an RS domain that is involved in binding:

Sequencing of small RNAs isolated from metazoan cells revealed low levels of short, capped RNAs transcribed from both the sense and antisense strands of DNA. What is the term used to describe the fact that the majority of the metazoan genome is transcribed?

The consensus sequence for poly(A) addition is

Histone mRNAs lack

Which process involves two transesterification reactions?

Splice sites in pre-mRNA are marked by two universally conserved sequences located

Splicing joins

The branch-point A residue involved in lariat formation is part of the

Indicate the order in which the following steps occur in the production of a mature mRNA.

Components of the spliceosome include

This type of RNA functions in the removal of introns from pre-RNAs.

In birds, a gene encoding a Ca -activated K channel is expressed in auditory hair cells as multiple mRNAs, which encode for proteins that open at different Ca concentrations. The Ca concentration at which the channel opens allows these cells to respond to different sound frequencies. Which one of the following would explain the appearance of the various isoforms of this particular channel?

Sex-lethal protein in Drosophila can best be described as a(n)

Which of the following does not require enzymes?

RNA editing is

The export of mRNAs outside the nucleus requires several proteins that are post-translationally modified by:

Some have evolved a constitutive transport element within their genome, which allows for the export of unspliced RNAs into the cytoplasm.

Which of these events does not occur within the nucleus?

Which type of RNA participates in nuclear export of mRNA?

Transport of unspliced HIV mRNA from the nucleus to the cytoplasm of host cells is promoted by a virus-encoded protein named

The RISC complex contains which one of the following proteins?

Knocking out the dicer gene in mammals would lead to a loss of:

You are using a variety of techniques to study how the RISC complex differs between siRNAs and miRNAs and have found that what distinguishes an RISC complex containing an siRNA from one containing an miRNA is that:

The mammalian target of rapamycin (mTOR) complex 1 plays an integral role in all but one of these processes. Which one is mTOR not directly involved in?

The cell contains numerous organelles, all with specific functions, but the one that plays a key role in the digestion of ribosomes, mitochondria, and other organelles is the:

Which of the following regarding the P-body is FALSE?

Cells use several ways to avoid the translation of improperly processed mRNA molecules. ____ is(are) considered mRNA surveillance mechanisms.

MicroRNAs play a key role in which of the following?

Which of the following does not take part in the degradation process of eukaryotic mRNAs?

Which of the following are NOT found within the nucleus?

Synthesis of pre-rRNA occurs in the

The 45S pre-rRNA molecule

A ribozyme is an RNA sequence