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Questions and Answers

What can result from an abnormal initiation of tooth development?

  • Malformation of the jaw
  • Formation of supernumerary teeth (correct)
  • Absence of dental pulp
  • Change in tooth color
  • What is the role of the inner enamel epithelium during histodifferentiation?

  • It secretes osteodentin
  • It initiates the proliferation of the enamel organ
  • It influences the adjacent cells to differentiate (correct)
  • It forms the dentin matrix directly
  • What disturbance occurs during proliferation that can affect tooth development?

  • Incorrect differentiation of ameloblasts
  • Random growth of teeth
  • Insufficient blood supply
  • Disruption in enamel matrix formation (correct)
  • What is osteodentin?

    <p>Dentin formed by improperly differentiated odontoblasts (D)</p> Signup and view all the answers

    What is the purpose of morphodifferentiation in tooth development?

    <p>Determining the morphological form and shape of the tooth (D)</p> Signup and view all the answers

    How does vitamin A deficiency affect tooth development?

    <p>It disrupts the differentiation of ameloblasts (D)</p> Signup and view all the answers

    Which teeth are most commonly associated with supernumerary teeth?

    <p>Mesiodens (C)</p> Signup and view all the answers

    When does reciprocal induction occur during tooth development?

    <p>In the bell stage of histodifferentiation (D)</p> Signup and view all the answers

    What is amelogenesis?

    <p>The growth of enamel on teeth (D)</p> Signup and view all the answers

    During which stage of tooth development does amelogenesis begin?

    <p>Advanced bell stage (D)</p> Signup and view all the answers

    Which type of proteins primarily make up the organic matrix of enamel?

    <p>Noncollagenous proteins (C)</p> Signup and view all the answers

    What is the primary role of amelogenins in enamel formation?

    <p>Regulate the growth and thickness of enamel (C)</p> Signup and view all the answers

    What occurs during the secretory phase of enamel formation?

    <p>Secretion of matrix proteins by ameloblasts (A)</p> Signup and view all the answers

    What is the function of matrix vesicles during the maturative phase?

    <p>To initiate crystal formation in preformed organic matrix (B)</p> Signup and view all the answers

    What is the primary function of the stellate reticulum in the enamel organ?

    <p>To act as a shock absorber and protect enamel-forming cells (D)</p> Signup and view all the answers

    Which structure forms a knob-like enlargement toward the dental papilla?

    <p>Primary enamel knot (D)</p> Signup and view all the answers

    Which of the following proteins is classified under nonamelogenins?

    <p>Enamelin (A)</p> Signup and view all the answers

    During which stage does the enamel organ take on a bell shape?

    <p>Bell stage (A)</p> Signup and view all the answers

    How is enamel thickness primarily regulated during enamel formation?

    <p>By the regulation of amelogenins (D)</p> Signup and view all the answers

    What is the role of stratum intermedium in the enamel organ?

    <p>To regulate the formation of enamel (C)</p> Signup and view all the answers

    What happens to the stellate reticulum before the formation of enamel?

    <p>It collapses to provide nutrition (A)</p> Signup and view all the answers

    What type of cells make up the inner enamel epithelium before the formation of enamel?

    <p>Columnar cells (C)</p> Signup and view all the answers

    What is the appearance of the cells in the center of the stellate reticulum?

    <p>Star-shaped (B)</p> Signup and view all the answers

    What defines the dental sac during the cap stage?

    <p>It appears more condensed and fibrous (A)</p> Signup and view all the answers

    What is the primary source of the posterior one-third of the tongue?

    <p>Third and fourth pharyngeal arches (D)</p> Signup and view all the answers

    What is the role of enamel on the teeth?

    <p>Covers and protects underlying tissues (B)</p> Signup and view all the answers

    At approximately what stage of embryo development does enamel formation begin?

    <p>18 weeks (D)</p> Signup and view all the answers

    What is the maximum thickness of enamel found over molars?

    <p>2.6 mm (A)</p> Signup and view all the answers

    Which component of the tongue is indicated by the terminal sulcus?

    <p>Line of fusion between anterior and posterior thirds (D)</p> Signup and view all the answers

    What primarily determines the color and translucency of enamel?

    <p>Underlying dentin color (B)</p> Signup and view all the answers

    Which characteristic best describes enamel?

    <p>Hardest calcified tissue (D)</p> Signup and view all the answers

    What is the primary component of enamel that contributes to its hardness?

    <p>Mineral salts (C)</p> Signup and view all the answers

    What disturbance can occur during morphodifferentiation affecting the crown of a tooth?

    <p>Supernumerary cusp formation (C)</p> Signup and view all the answers

    What does apposition primarily involve in tooth development?

    <p>Deposition of the matrix of dental hard tissues (D)</p> Signup and view all the answers

    What term describes disturbances involving the formation of the enamel matrix?

    <p>Hypoplasia (D)</p> Signup and view all the answers

    Which of the following structures derives from the fronto-nasal process during face development?

    <p>Nasal cavities (D)</p> Signup and view all the answers

    What do hypocalcification and hypomineralization indicate in tooth development?

    <p>Problems with calcification or mineralization (A)</p> Signup and view all the answers

    From which embryonic structure do the paired mandibular prominences develop?

    <p>First branchial arch (B)</p> Signup and view all the answers

    What is the primary focus of disturbances occurring during the morphodifferentiation process?

    <p>Morphology of the tooth crown or root (C)</p> Signup and view all the answers

    At what embryonic week does the development of face structures mainly occur?

    <p>4th week (C)</p> Signup and view all the answers

    Where is cementum thinnest?

    <p>At the cementoenamel junction (A)</p> Signup and view all the answers

    What characterizes acellular afibrillar cementum?

    <p>Contains mineralized ground substance and no cells or fibers (A)</p> Signup and view all the answers

    Which type of cementum is primarily found in the apical third of roots?

    <p>Cellular mixed stratified cementum (A)</p> Signup and view all the answers

    What is the role of incremental lines of Salter in cementum?

    <p>To indicate mineralized areas with less collagen (B)</p> Signup and view all the answers

    What happens at the cementoenamel junction in 60% of teeth?

    <p>Cementum overlaps enamel (B)</p> Signup and view all the answers

    Which type of cementum is formed by cementoblasts and lacks extrinsic collagen fibers?

    <p>Cellular intrinsic fiber cementum (D)</p> Signup and view all the answers

    Why is cementum resistant to resorption compared to bone?

    <p>It is avascular and lacks blood supply (A)</p> Signup and view all the answers

    What is a characteristic of acellular extrinsic fiber cementum?

    <p>Is composed mostly of Sharpey's fibers (D)</p> Signup and view all the answers

    Flashcards

    Stellate Reticulum

    Star-shaped, branching network of cells found in the enamel organ's center; acts as a shock absorber protecting enamel-forming cells.

    Primary Enamel Knot

    Knob-like structure on the inner surface of the enamel organ; a key area directing enamel formation.

    Enamel Cord

    Vertical extension of the primary enamel knot, positioned centrally in the enamel organ.

    Ameloblasts

    Tall columnar cells that differentiate from inner enamel epithelium to form enamel.

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    Stratum Intermedium

    Layer between inner and stellate reticulum in the enamel organ, aiding enamel formation.

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    Inner Enamel Epithelium

    Single layer of columnar cells that develop into ameloblasts and form enamel.

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    Outer Enamel Epithelium

    Cuboidal cells forming the outermost layer of the enamel organ, initially smooth.

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    Glycosaminoglycans

    Substances synthesized by cells in the center of the enamel organ, absorbing water and causing stretching of intercellular bridges in the stellate reticulum.

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    Supernumerary Teeth

    Extra teeth that form due to abnormal tooth initiation.

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    Mesiodens

    A common type of supernumerary tooth located between the upper central incisors.

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    Proliferation (in tooth development)

    Increase in the number of tooth cells to form the tooth crown's size and shape.

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    Histodifferentiation

    The process where cells change their structure and function to create specific tooth tissues (e.g. enamel, dentin).

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    Odontoblasts

    Cells that create dentin, the hard tissue of the tooth.

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    Reciprocal Induction

    Inner enamel epithelium influencing dental papilla cells and vice versa, leading to proper tooth development.

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    Osteodentin

    Dentin formed due to abnormal odontoblast differentiation, often caused by vitamin deficiency.

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    Morphodifferentiation

    The stage of tooth development where the basic shape of the tooth is established. It's influenced by the interaction and differentiation of cells in the enamel organ and dental papilla.

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    Supernumerary Cusp

    An extra cusp on a tooth, caused by disturbances in morphodifferentiation during crown formation. This can lead to an unusual shape or number of cusps.

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    Peg-shaped Tooth

    A thin, pointed tooth caused by disturbances in morphodifferentiation during crown formation. It usually happens when the crown doesn't develop properly.

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    Dilaceration

    An abnormal bend or curve in the root of a tooth, often caused by trauma during tooth development.

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    Apposition

    The process of adding layers of mineralized tissue to create the final shape of a tooth. It involves the deposition of enamel and dentin in a rhythmic pattern.

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    Hypoplasia

    A disturbance in the formation of the enamel matrix of a tooth, leading to an incomplete or thinner enamel layer.

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    Hypocalcification

    A disturbance in the mineralization process of the enamel matrix, resulting in a weaker, more porous enamel.

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    Hypomineralization

    Similar to hypocalcification, a disturbance in the mineralization process of the enamel matrix, leaving the enamel weaker and more prone to decay.

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    Posterior 1/3 of tongue

    The back portion of the tongue, formed by the merging of the 3rd and 4th pharyngeal arches during development. It overgrows the 2nd arch, forming the back of the tongue.

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    Terminal Sulcus

    A groove on the tongue's surface that marks the boundary between the anterior 2/3 (formed by the 1st arch) and the posterior 1/3 (formed by the 3rd and 4th arches).

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    Enamel: Introduction

    Enamel is the hard, outer layer of the tooth crown, providing protection. It's formed by ectoderm and begins development around 18 weeks of embryo age.

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    Enamel: Physical Properties

    Enamel is incredibly hard, primarily due to its high mineral content and crystalline structure. It's vital for resisting the forces of chewing.

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    Enamel: Thickness

    Enamel thickness varies: thickest on the biting surfaces (cusps/incisal edge) and thinnest at the tooth's neck, grooves, or pits.

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    Enamel: Color & Translucency

    Enamel is normally translucent, allowing the yellow dentin underneath to show through. Greyish enamel means it's more opaque. Yellow teeth often have thinner, translucent enamel.

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    Enamel: Hardness

    Enamel is the hardest tissue in the human body, even harder than bone. This is due to its densely packed mineral structure.

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    Enamel: Composition

    Enamel is about 96% mineral content, primarily hydroxyapatite crystals, and only about 4% organic material. This high mineral content explains its strength.

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    Hydroxyapatite Fluoride

    Fluoride ions replace some of the hydroxyl ions in the hydroxyapatite structure of tooth enamel, making it more resistant to acid attacks.

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    Calcium Hydroxyapatite

    The main mineral component of tooth enamel, providing its hardness and resistance.

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    Amelogenesis

    The process of forming enamel on teeth; starts during the advanced bell stage of tooth development, after dentin formation.

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    What are the two main phases of Amelogenesis?

    The process of enamel formation occurs in two phases: the secretory phase, where the organic matrix is laid down, and the maturative phase, where enamel crystals mature and harden.

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    Enamel Proteins

    The organic matrix of enamel is composed of noncollagenous proteins, mainly amelogenins and nonamelogenins.

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    Amelogenins

    Major enamel proteins that regulate enamel growth and thickness, making up about 90% of the enamel protein matrix.

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    Non-Amelogenins

    A group of smaller enamel proteins, including enamelin, amelin, sheathlin, and tuftelin, that play a role in enamel structure and function.

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    Cementum Thickness

    Cementum is thinnest at the cementoenamel junction (where enamel and cementum meet) and thickest towards the root's tip (apex).

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    Cementocytes

    These are cells embedded in cementum, but they are either inactive or in a state of degeneration.

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    Acellular Afibrillar Cementum

    This type of cementum lacks cells and collagen fibers, only containing mineralized ground substance. It's found in the crown area of the tooth.

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    Acellular Extrinsic Fiber Cementum

    Mostly made up of Sharpey's fibers, which are dense bundles of collagen fibers embedded in cementum. It's located in the cervical third of the root.

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    Cellular Mixed Stratified Cementum

    This type of cementum contains both extrinsic and intrinsic collagen fibers and cells. It's found in the apical third of roots, at the furcations, and the root tip.

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    Cellular Intrinsic Fiber Cementum

    This type of cementum has cells but no extrinsic (outside) fibers. It's produced by cementoblasts and fills in spaces left by resorption.

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    Incremental Lines of Salter

    Highly mineralized areas in cementum with a lower amount of collagen and a greater amount of ground substance. They represent periods of rapid cementum growth.

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    Cementodentinal Junction

    The area where dentin meets cementum. It's smooth in permanent teeth and scalloped in deciduous teeth. A layer called intermediate cementum is located between them.

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    Study Notes

    Germ Layer Formation

    • Germ layers form from the morula, a ball of cells, in mammals after fertilization.
    • The major portion of the egg cell mass forms extraembryonic membranes and structures like the placenta.
    • The inner cell mass, embryoblast, separates into two layers: the epiblast and hypoblast.
    • Only the epiblast forms the embryo.
    • The hypoblast and other cells form supporting tissues like the placenta.
    • The process of formation of the three germ layers is called gastrulation.
    • Gastrulation involves the formation of a primitive streak and primitive node.
    • Epiblast cells divide and migrate through the primitive streak, replacing hypoblast cells and forming endoderm.
    • Cells between the epiblast and endoderm form the mesoderm.
    • Cells remaining in the epiblast form the ectoderm, completing the three germ layers.
    • The notochord is a solid cord formed by prenotochordal cells between the ectoderm and endoderm that reaches the prochordal plate.
    • The notochord forms the basis for the vertebral column in later development, persisting partially as the nucleus pulposus of the vertebral disks.

    Neural Tube Formation

    • In the midline, the ectoderm over the notochord thickens to form the neural plate.
    • The neural plate furrows to form the neural groove.
    • Neural folds on either side of the groove elevate, fuse, and form the neural tube.
    • Neural crest cells develop from the neural folds and migrate to other locations in the body, contributing to a variety of tissues like odontoblasts, melanocytes, and ganglia.

    Pharyngeal Arches

    • Pharyngeal arches develop progressively, contributing to the face and neck.
    • Each arch contains skeletal elements, arteries, muscles, and nerves.
    • The first arch (mandibular arch) gives rise to muscles of mastication, mylohyoid, anterior belly of digastric, tensor veli palatini and tensor tympani.
    • It also gives rise to Meckel's cartilage(malleus, incus, anterior ligament of malleus, sphenomandibular ligament.)
    • The mandibular nerve supplies muscles of mastication; chorda tympani and maxillary artery are also part of the first arch.
    • The second arch (hyoid arch) gives rise to muscles of facial expression, posterior belly of digastric, stylohyoid, stapedius.
    • It also gives rise to Reichert's cartilage(stapes, stylohyoid ligament, lesser cornu and upper half of body of hyoid bone, styloid process).
    • The facial nerve and stapedial artery are part of the second arch.
    • Derivatives of the third arch are the stylopharyngeus muscle and the greater horn and lower part of the hyoid bone.
    • The glossopharyngeal nerve supplies the third arch.
    • The fourth and sixth arches give rise to parts of the larynx, including the cricothyroid, levator palatine, constrictor of pharynx, intrinsic muscle of larynx, laryngeal cartilages.
    • The superior laryngeal (4th) and recurrent laryngeal nerve(6th) innervate those structures.
    • Ectodermal clefts and endodermal pouches from between the arches develop into various structures including the auditory tube, palatine tonsils, and parathyroid glands.

    Development of Teeth

    • Teeth form from ectoderm and ectomesenchyme.
    • At 37 days, a thickened band of epithelium appears in the developing jaws and is shaped like a horseshoe.
    • The primary epithelial band gives rise to other subdivisions including the dental lamina.
    • The initiation of tooth development starts with the formation of localized thickenings or placodes in the primary epithelial bands.
    • A series of 10 local thickenings occur in the dental lamina, and each placode forms one deciduous tooth.
    • Tooth development proceeds through the bud, cap, and bell stages.
    • The bud stage is characterized by the formation of a tooth bud as the epithelial cells proliferate into the ectomesenchyme of the jaw.
    • The supporting ectomesenchymal cells densely pack around the epithelial bud.
    • This results in condensation of ectomesenchyme.
    • The cap stage involves the formation of dental papilla.
    • The enamel organ and the dental papilla form the tooth germ.
    • The bell stage sees the enamel organ taking a bell shape.
    • Cells that will form the hard tissues of the crown (ameloblasts(forming enamel) and odontoblasts (forming dentin)) acquire histodifferentiation at this stage.
    • The enamel organ has four layers during the bell stage: inner enamel epithelium, stratum intermedium, the stellate reticulum, and outer enamel epithelium.
    • The cells in the center of the concavity of the enamel organ form a structure called the primary enamel knot. The enamel knot extends vertically across the center of the enamel organ -- called the enamel cord.
    • Cells of the dental papilla appear crowded at the cap stage.
    • The dental papilla and dental sac become more vascular at the cap stage.
    • The dental sac is more condensed and fibrous in the cap stage.
    • Mineralization starts during the advanced bell stage, and root formation commences.
    • Mineralisation involves the differentiation of the peripheral cells of the dental papilla into odontoblasts and the secretion of organic matrix.
    • The line separating the odontoblasts and the inner enamel epithelium forms the dentinoenamel junction.
    • Enamel formation happens in two phases: secretory and maturative.
    • The secretory phase involves protein secretion by ameloblasts (forming enamel); the maturative phase involves reabsorption of proteins and water, and mineral secretion.
    • The epithelial root sheath forms the epithelial diaphragm prior to root formation.
    • The proliferation of cells of the epithelial diaphragm is accompanied by the proliferation of connective tissue adjacent to the diaphragm.
    • Tooth development can begin during the advanced bell stage, characterized by the start of mineralization and root formation and ends with the final shape of the crown and also involves root formation.
    • The epithelial diaphragm divides into a network of epithelial strands and the surrounding connective tissue proliferates.
    • Additional structures like the periodontal ligament and alveolar bone form.
    • The most common teeth missing, or incomplete, are the upper lateral incisors, third molars, and lower second premolars.

    Development of Face and Palate

    • The face is derived from the fronto-nasal process, and first pharyngeal arch of each side.
    • The first pharyngeal arch divides into the maxillary and mandibular process.
    • The fronto-nasal process forms the forehead and bridge of the nose.
    • The paired maxillary prominences form the upper cheek, upper lip, and maxilla, including most of the secondary palate.
    • The paired mandibular prominences form the area of the chin and lower lip.
    • The lateral nasal processes form the alae of the nose and
    • The fusion of medial nasal processes forms the intermaxillary segment(philtrum of lip, premaxillary portion of maxilla with the upper four incisors, and the associated gums).
    • The palate starts to develop at the end of the 5th week, which finishes by the 12th week with the primary palate forming in the beginning of the 6th week by the intermaxillary segment as median palatine process.
    • The secondary palate forms from the internal aspects of the maxillary prominences as lateral palatine processes.
    • Lateral palatine processes grow medially and fuse, merging to form the hard palate and fuse with the posterior part of the primary palate and nasal septum.
    • The fusion of the lateral palatine processes with the nasal septum begins anteriorly during the 9th week and extends posteriorly to complete by the 12th week.

    Development of the Tongue

    • The tongue forms from the first, second, third, and fourth pharyngeal arches.
    • The anterior two-thirds of the tongue form from the fusion of the tuberculum impar and the two lateral tongue swellings.
    • The posterior one-third of the tongue forms from the copula.
    • The swellings from the floor of the third and fourth pharyngeal arches overgrow the second arch and fuse with the anterior two-thirds of the tongue.
    • The posterior one-third of the tongue is derived from the third and fourth arches.
    • The intrinsic musculature also is derived from occipital myotomes.
    • The line of fusion of the anterior two-thirds and posterior one-third of the tongue is indicated by the terminal sulcus.

    Enamel

    • Enamel is the hard, vitreous covering of the crown of the tooth.
    • Enamel is made of hydroxyapatite crystals in a highly organized structure.
    • Enamel forms quickly in the advanced bell stage once crown and the first layer of dentine forms..
    • Enamel is composed of two phases; a secretory phase and a maturative phase.
    • Enamel is made of amelogenins and nonamelogenins.
    • Enamel is usually translucent and pale yellow.
    • Enamel is thickest at the cusp, thinning to a knife edge at the neck of the tooth.

    Dentin

    • Dentin is the primary bulk of the tooth.
    • Dentin surrounds the pulp cavity.
    • Dentin supports the enamel.
    • Dentin is composed of 70% inorganic matter, 20% organic matter, and 10% water.
    • It is harder than bone and cementum but softer than enamel.
    • The primary dentin, secondary dentin, and tertiary dentin are types of dentin.
    • Dentin has a tubular structure with odontoblastic processes, nerves, and fluid.
    • Dentin is slightly harder in the crown than near the pulp on its periphery.
    • Dentin is harder in the primary teeth than the permanent teeth.

    Cementum

    • Cementum is a mineralized connective tissue that covers the anatomical roots of human teeth.
    • It begins at the cementoenamel junction and extends to the apex of the roots.
    • It serves as a medium for attachment of collagen fibers.
    • It is responsible for adapting to the functional needs of the teeth.
    • There are two types of cementum: acellular and cellular.
    • Acellular cementum has no cells within it, while cellular cementum does.
    • Acellular cementum is found in the coronal third of the roots.
    • Cellular cementum is in the apical third.
    • Cementum is highly resistant to resorption and is less hard than dentin.
    • It is a light yellow color and has less luster compared to enamel. It is semi-permeable to various substances.
    • It consists of 45-50% inorganic material, and 50-55% organic material. It has high fluoride content because of this.
    • The uncalcified matrix is called cementoid. This is highly organized vs. random.
    • The embedded portions of the fibers are called Sharpey's fibers. These fibers attach the tooth to surrounding bone.
    • The cementodentinal junction is predominantly smooth in permanent teeth and scalloped in deciduous teeth.

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