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Questions and Answers
What type of culture is favoured when the ratio of auxin to cytokinin is high?
What type of culture is favoured when the ratio of auxin to cytokinin is high?
What is the purpose of sterilizing the media in plant tissue culture?
What is the purpose of sterilizing the media in plant tissue culture?
What equipment is required for sterilizing glassware and metallic equipment?
What equipment is required for sterilizing glassware and metallic equipment?
What is the ideal temperature for sterilizing media in an autoclave?
What is the ideal temperature for sterilizing media in an autoclave?
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Which of the following ratios of auxin to cytokinin favours intermediate callus production?
Which of the following ratios of auxin to cytokinin favours intermediate callus production?
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What is the primary reason for sterilizing living plant materials from the environment?
What is the primary reason for sterilizing living plant materials from the environment?
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What is the role of a gelling agent in plant tissue culture?
What is the role of a gelling agent in plant tissue culture?
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What is the purpose of using a growth room with controlled temperature and light intensity?
What is the purpose of using a growth room with controlled temperature and light intensity?
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What is the composition of solid and liquid media in plant tissue culture?
What is the composition of solid and liquid media in plant tissue culture?
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Why are explants sometimes placed directly into a sterile liquid medium?
Why are explants sometimes placed directly into a sterile liquid medium?
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Study Notes
Plant Growth Regulators in Tissue Culture
- Auxins and cytokinins are the most commonly used plant growth regulators in plant tissue culture.
- They are typically used together to achieve specific culture types.
- The ratio of auxin to cytokinin determines the type of culture established or regenerated.
Effects of Auxin to Cytokinin Ratio
- High auxin to cytokinin ratio: favours root formation.
- High cytokinin to auxin ratio: favours shoot formation.
- Intermediate ratio: favours callus production.
Sterilisation of Media
- Sterilization method for large amounts of media: ISI mark Autoclave at 1210C.
- Sterilization method for small amounts of media: domestic pressure cookers.
- Sterilization method for glassware and metallic equipment: Hot air oven with adjustable tray.
Tissue Culture
- Tissue is grown in sterile containers such as petri dishes or flasks in a controlled growth room with regulated temperature and light intensity.
- Living plant materials are naturally contaminated with microorganisms on their surfaces, so they are sterilized in chemical solutions before sampling.
Preparation of Explants
- Sterile explants are taken from sterilized plant materials.
- Sterile explants are placed on the surface of a sterile solid culture medium or directly into a sterile liquid medium (for cell suspension cultures).
Culture Media
- Culture media are composed of inorganic salts plus organic nutrients, vitamins, and plant hormones.
- Solid media are prepared by adding a gelling agent (usually purified agar) to liquid media.
- Liquid media are used for cell suspension cultures.
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Description
Learn about the role of auxins and cytokinins in plant tissue culture, including how their ratio affects root and shoot formation, and the importance of sterilization of media.
