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Questions and Answers
Describe the physiological effects of cytokinins.
Describe the physiological effects of cytokinins.
Cytokinins promote cell division, enhance shoot formation, delay senescence, and stimulate nutrient mobilization.
Explain why a brief interruption of the critical dark period with red light inhibits flowering in short-day plants.
Explain why a brief interruption of the critical dark period with red light inhibits flowering in short-day plants.
Red light interrupts the dark period, affecting the perception of day length and thereby inhibiting flowering.
Describe the role of light, ABA, and gibberellins in the regulation of seed germination.
Describe the role of light, ABA, and gibberellins in the regulation of seed germination.
Light stimulates germination in some seeds, ABA inhibits it, and gibberellins promote germination by breaking seed dormancy.
Write an explanatory note on the classification of carbohydrates.
Write an explanatory note on the classification of carbohydrates.
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Provide a brief classification of amino acids based on their functional groups with the help of a flowchart.
Provide a brief classification of amino acids based on their functional groups with the help of a flowchart.
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Explain how pentoses or hexoses undergo cyclisation in aqueous solution.
Explain how pentoses or hexoses undergo cyclisation in aqueous solution.
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Differentiate between competitive and non-competitive inhibition of enzyme-catalyzed reactions.
Differentiate between competitive and non-competitive inhibition of enzyme-catalyzed reactions.
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Which of the following are examples of vitamins as coenzymes? (Select all that apply)
Which of the following are examples of vitamins as coenzymes? (Select all that apply)
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Explain the CAM pathway.
Explain the CAM pathway.
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What is the Michaelis-Menten constant?
What is the Michaelis-Menten constant?
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Study Notes
Cytokinins: Physiological Effects
- Stimulate cell division and differentiation.
- Promote growth of lateral buds and shoots.
- Delay leaf senescence (aging).
- Influence apical dominance by interacting with auxins.
- Involved in chloroplast development and photosynthesis.
Red Light and Flowering in Short-Day Plants
- Short-day plants flower when the night period exceeds a critical length.
- Red light (R) is perceived by phytochrome, a photoreceptor.
- Brief exposure to red light during the dark period converts Pr to Pfr.
- Pfr inhibits flowering. A subsequent far-red (FR) pulse reverses this effect.
- This interruption shifts the plant's perception of night length, preventing flowering.
Seed Germination Regulation
- Light: Light signals (e.g., red light) can initiate germination in many species through phytochrome action.
- ABA (Abscisic Acid): Acts as a germination inhibitor, maintaining seed dormancy. Its levels decline during germination.
- Gibberellins: Stimulate germination by promoting enzyme production for breaking down stored food reserves. They counteract ABA's inhibitory effects.
Carbohydrate Classification
- Monosaccharides: Simple sugars (e.g., glucose, fructose, galactose).
- Disaccharides: Two monosaccharides linked (e.g., sucrose, lactose, maltose).
- Oligosaccharides: Short chains of 3-10 monosaccharides.
- Polysaccharides: Long chains of monosaccharides (e.g., starch, cellulose, glycogen). Classification based on monomer type and linkage.
Amino Acid Classification (Flowchart Focus)
- Flowchart would show branching based on the nature of the side chain (R group).
- Branches would include categories such as: non-polar aliphatic, aromatic, polar uncharged, polar charged (acidic and basic). Specific amino acid examples should be given for each category (e.g., Glycine, Phenylalanine, Serine, Aspartic acid, Lysine).
Cyclization of Pentoses and Hexoses
- In aqueous solution, pentoses (5-carbon sugars) and hexoses (6-carbon sugars) exist in equilibrium between open-chain and cyclic forms.
- Cyclization involves the reaction of the carbonyl group (aldehyde or ketone) with a hydroxyl group within the same molecule, forming a hemiacetal or hemiketal ring.
- Examples include the formation of the pyranose (six-membered ring) and furanose (five-membered ring) forms of glucose and fructose, respectively. The chair and boat conformations of the cyclic structures should be mentioned.
Enzyme Inhibition: Competitive vs. Non-Competitive
- Competitive Inhibition: Inhibitor resembles substrate and competes for the enzyme's active site. Increased substrate concentration can overcome inhibition. Km increases, Vmax remains constant.
- Non-Competitive Inhibition: Inhibitor binds to a site other than the active site, altering enzyme conformation. Substrate binding is unaffected, but enzyme activity is reduced. Km stays constant, Vmax decreases.
Vitamins as Coenzymes/CAM Pathway/Michaelis-Menten Constant (Short Notes)
- Vitamins as Coenzymes: Many vitamins function as coenzymes, non-protein organic molecules that assist enzyme function. Examples: Vitamin B1 (thiamine) in pyruvate dehydrogenase, Vitamin B2 (riboflavin) in FAD, Vitamin B3 (niacin) in NAD.
- CAM Pathway (Crassulacean Acid Metabolism): A photosynthetic adaptation in arid environments. CO2 uptake occurs at night, stored as malic acid. During the day, malic acid is decarboxylated to release CO2 for photosynthesis, minimizing water loss.
- Michaelis-Menten Constant (Km): Represents the substrate concentration at which the reaction rate is half the maximum velocity (Vmax). It reflects the enzyme's affinity for the substrate; a lower Km indicates a higher affinity.
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Description
Test your knowledge on the physiological effects of cytokinins, the role of red light in flowering for short-day plants, and the regulation of seed germination. This quiz covers key concepts and mechanisms in plant physiology, including the influence of hormones and light signals on plant growth and development.