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Questions and Answers
What is electroporation?
Which technique involves cutting pollen tubes after pollination?
Which technique can inject a variety of materials such as chromosomes, chloroplasts, and mitochondria?
Which technique involves introducing plant material into a buffer containing DNA and silicon carbide fibres?
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Which technique involves enclosing DNA in lipid vesicles?
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Which techniques have been successfully used to transform tobacco, maize, and rice?
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What is the mechanism of DNA transfer into the zygote?
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Study Notes
- Electroporation and microinjection are techniques used for genetic transformation in plant cells.
- Electroporation involves applying a high-voltage electrical pulse across platinum electrodes in the cuvette.
- Microinjection involves injecting foreign DNA into the nucleus of protoplasts using a holding pipette or injection pipette.
- Silicon carbide fibre technique involves introducing plant material into a buffer containing DNA and silicon carbide fibres, which penetrate the cell wall and plasma membrane.
- Liposome encapsulation involves enclosing DNA in lipid vesicles, which penetrate the protoplasts and release DNA for integration into the host genome.
- The pollen-tube pathway is a technique used to deliver foreign DNA into embryo sacs in plants.
- Pollen tubes are cut after pollination, and DNA is introduced at the cut surface.
- The mechanism of DNA transfer into the zygote is not yet established.
- Electroporation and microinjection have been successfully used to transform tobacco, maize, and rice.
- Microinjection can inject a variety of materials such as chromosomes, chloroplasts, and mitochondria.
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Description
Test your knowledge on the various techniques used for genetic transformation in plant cells with this quiz! From electroporation to pollen-tube pathway, this quiz covers the different methods used to introduce foreign DNA into plant cells. Learn about the advantages and disadvantages of each technique, as well as the plant species that have been successfully transformed using them. Challenge yourself with questions on microinjection, liposome encapsulation, and more in this informative quiz!
