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Questions and Answers
What is electroporation?
What is electroporation?
- Injecting foreign DNA into the nucleus of protoplasts
- Introducing plant material into a buffer containing DNA and silicon carbide fibres
- Applying a high-voltage electrical pulse across platinum electrodes in the cuvette (correct)
- Enclosing DNA in lipid vesicles
Which technique involves cutting pollen tubes after pollination?
Which technique involves cutting pollen tubes after pollination?
- Silicon carbide fibre technique
- Pollen-tube pathway (correct)
- Electroporation
- Microinjection
Which technique can inject a variety of materials such as chromosomes, chloroplasts, and mitochondria?
Which technique can inject a variety of materials such as chromosomes, chloroplasts, and mitochondria?
- Liposome encapsulation
- Silicon carbide fibre technique
- Electroporation
- Microinjection (correct)
Which technique involves introducing plant material into a buffer containing DNA and silicon carbide fibres?
Which technique involves introducing plant material into a buffer containing DNA and silicon carbide fibres?
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Which technique involves enclosing DNA in lipid vesicles?
Which technique involves enclosing DNA in lipid vesicles?
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Which techniques have been successfully used to transform tobacco, maize, and rice?
Which techniques have been successfully used to transform tobacco, maize, and rice?
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What is the mechanism of DNA transfer into the zygote?
What is the mechanism of DNA transfer into the zygote?
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Study Notes
- Electroporation and microinjection are techniques used for genetic transformation in plant cells.
- Electroporation involves applying a high-voltage electrical pulse across platinum electrodes in the cuvette.
- Microinjection involves injecting foreign DNA into the nucleus of protoplasts using a holding pipette or injection pipette.
- Silicon carbide fibre technique involves introducing plant material into a buffer containing DNA and silicon carbide fibres, which penetrate the cell wall and plasma membrane.
- Liposome encapsulation involves enclosing DNA in lipid vesicles, which penetrate the protoplasts and release DNA for integration into the host genome.
- The pollen-tube pathway is a technique used to deliver foreign DNA into embryo sacs in plants.
- Pollen tubes are cut after pollination, and DNA is introduced at the cut surface.
- The mechanism of DNA transfer into the zygote is not yet established.
- Electroporation and microinjection have been successfully used to transform tobacco, maize, and rice.
- Microinjection can inject a variety of materials such as chromosomes, chloroplasts, and mitochondria.
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